Proteomic analysis of Red Sea Conus taeniatus venom reveals potential biological applications

Fouda, Maged M. A.; Abdel-Wahab, Mohammed; Mohammadien, Amal; Germoush, Mousa O.; Sarhan, Moustafa

doi:10.1590/1678-9199-jvatitd-2021-0023

Cited by 2 publications

(1 citation statement)

References 70 publications

(85 reference statements)

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“…Another example of interest is gastropod Conus taeniatus, whose proteome was analysed by Fouda et al (2021). These authors matched the outcomes from MS/MS proteomics against a lineage-specific (Conus) database.…”

Section: Omics Studies Employed To Unravel Promising Bioactivesmentioning

confidence: 99%

Bioprospecting and marine ‘omics’: surfing the deep blue sea for novel bioactive proteins and peptides

Moutinho Cabral,

Gonçalves,

Grosso

et al. 2024

Front. Mar. Sci.

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The vast biological and biochemical diversity of the global ocean is the driver behind marine bioprospecting for novel bioproducts. As Marine Biotechnology is gaining momentum as one of the main pillars of the ‘Brue Growth’ revolution, the ability to screen for novel compounds of interest in species with little or no genomic resources is paramount. With this respect, proteins, which are easily metabolised, can be synthetised using convenient DNA recombinant methods and can easily be modified to better meet the needs of human society, making them prized targets. Evidently, proteins that hold natural bioactivity and specificity such as toxins and other venom components, have long captured the focus of biotechnologists, leading to the merger between environmental omics and toxinology termed as ‘venomics’. Indeed, bioactive proteins such as conopeptides, conotoxins, turripeptides and others are long deemed important subjects of research. Even though current mainstream paradigms set the focus on secondary metabolites from marine organisms, transcriptomics and proteomics approaches and their combination are rising strategies for screening for thousands of proteins and peptides in non-conventional biological models, emphasising, but not limited to, marine invertebrate animals due to their abundance, biodiversity and uncanny biochemical strategies to cope with selective pressure in literally every known marine habitat. Untargeted approaches, such as RNA-Seq – based transcriptomics and tandem mass spectrometry – based proteomics, can circumvent limitations related with absent or reduced genomic annotation. The present review will outline the main contributions of ‘omics’ and computational approaches for bioprospecting for proteinaceous marine bioactives. Despite the relatively low number of ‘omics’ studies with the main purpose of discover novel compounds, there is already important literature showcasing pipelines and approaches for revolutionising the exploration of the ocean.

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Section: Omics Studies Employed To Unravel Promising Bioactivesmentioning

confidence: 99%

Bioprospecting and marine ‘omics’: surfing the deep blue sea for novel bioactive proteins and peptides

Moutinho Cabral,

Gonçalves,

Grosso

et al. 2024

Front. Mar. Sci.

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Expansion and Neofunctionalization of Actinoporin-like Genes in Mediterranean Mussel (Mytilus galloprovincialis)

Koritnik

Gerdol

Šolinc

et al. 2022

Genome Biology and Evolution

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Pore-forming toxins are an important component of the venom of many animals. Actinoporins are potent cytolysins that were first detected in the venom of sea anemones; however, they are occasionally found in animals other than cnidarians and are expanded in a few predatory gastropods. Here, we report the presence of 27 unique actinoporin-like genes with monophyletic origin in Mytilus galloprovincialis, which we have termed mytiporins. These mytiporins exhibited a remarkable level of molecular diversity and gene presence-absence variation, which warranted further studies aimed at elucidating their functional role. We structurally and functionally characterized mytiporin-1 and found significant differences from the archetypal actinoporin fragaceatoxin C. Mytiporin-1 showed weaker permeabilization activity, no specificity towards sphingomyelin, and weak activity in model lipid systems with negatively charged lipids. In contrast to fragaceatoxin C, which forms octameric pores, functional mytiporin-1 pores on negatively charged lipid membranes were hexameric. Similar hexameric pores were observed for coluporin-26 from Cumia reticulata and a conoporin from Conus andremenezi. This indicates that also other molluscan actinoporin-like proteins differ from fragaceatoxin C. Although the functional role of mytiporins in the context of molluscan physiology remains to be elucidated, the lineage-specific gene family expansion event that characterizes mytiporins indicates that strong selective forces acted on their molecular diversification. Given the tissue distribution of mytiporins, this process may have broadened the taxonomic breadth of their biological targets, which would have important implications for digestive processes or mucosal immunity.

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Proteomic analysis of Red Sea Conus taeniatus venom reveals potential biological applications

Cited by 2 publications

References 70 publications

Bioprospecting and marine ‘omics’: surfing the deep blue sea for novel bioactive proteins and peptides

Bioprospecting and marine ‘omics’: surfing the deep blue sea for novel bioactive proteins and peptides

Expansion and Neofunctionalization of Actinoporin-like Genes in Mediterranean Mussel (Mytilus galloprovincialis)

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