Proteomic analysis of rice mutants susceptible to Magnaporthe oryzae

Abstract

To identify genes involved in rice Pi5-mediated disease resistance to Magnaporthe oryzae, we compared the proteomes of the RIL260 rice strain carrying the Pi5 resistance gene with its susceptible mutants M5465 and M7023. Proteins were extracted from the leaf tissues of both RIL260 and the mutant lines at 0, 24, and 48 h after M. oryzae inoculation and separated by two-dimensional polyacrylamide gel electrophoresis (2-DE). Matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS) analysis identified eight proteins that were differently expressed between the resistant and susceptible plants (three down-and five up-regulated proteins in the mutants). The down-regulated proteins included a triosephosphate isomerase (spot no. 2210), a 2,3-bisphosphoglycerate-independent phosphoglycerate mutase (no. 3611), and an unknown protein (no. 4505). In addition, the five up-regulated proteins in the mutants were predicted to be a fructokinase I (no. 313), a glutathione S-transferase (no. 2310), an atpB of chloroplast ATP synthase (no. 3616), an aminopeptidase N (no. 3724), and an unknown protein (no. 308). These results suggest that proteomic analysis of rice susceptible mutants is a useful method for identifying novel proteins involved in resistance to the M. oryzae pathogen.

Transcriptional Profiling of Rice Early Response to Magnaporthe oryzae Identified OsWRKYs as Important Regulators in Rice Blast Resistance

“…Transgenic plants overexpressing OsWRKY13 , OsWRKY31 , OsWRKY45 , and OsWRKY53 showed enhanced resistance to M. oryzae , indicating their important roles in rice blast resistance [17]–[21]. Large-scale approaches have also been used to study rice early response to M. oryzae , including EST sequencing [22], robust-long serial analysis of gene expression [23], proteomics [24], [25], and microarrays [26]. A number of genes potentially involved in rice blast resistance were identified from these studies.…”

Proteomics of Rice—Magnaporthe oryzae Interaction: What Have We Learned So Far?

“…In the comparative proteome study of rice strains carrying the Pi5 R gene with susceptible mutants against challenge of M. oryzae , eight proteins were differentially regulated between resistant and susceptible plants. Among them, an abundance of three proteins were found to be increased in resistance cultivar, including a triosephosphate isomerase, a 2,3-bisphosphoglycerate-independent phosphoglycerate mutase, and an unknown protein, while five downregulated proteins included a fructokinase I, a GST, an atpB of chloroplast ATP synthase, an aminopeptidase N, and an unidentified protein (Ryu et al, 2009). A comparative proteome study was performed between two incompatible interactions ( M. oryzae vs. ZTS rice leaf sheaths expressing whole plant-specific resistance [WPSR] and resistant rice ZTR (carrying Pi-zt ) and one compatible interaction ( M. oryzae vs. ZTS rice leaf sheaths in which WPSR was suppressed by ABA).…”

Korean plant proteomics: pioneers in plant stress physiology

“…As a major fungal pathogen, the interaction between Magnaporthe oryzae inducing rice blast disease and rice plants was reported by Jung et al (2005), Kim et al (2009), and Ryu et al (2009). In the previous study though secretome analysis in rice supension-cultured cells treated with M. oryzae or its elicitor, 21 differential protein spots regulated by fungus and its elicitor were identified and it was revealed that most of them are involved in defense response against pathogens (Kim et al 2009).…”

“…In the previous study though secretome analysis in rice supension-cultured cells treated with M. oryzae or its elicitor, 21 differential protein spots regulated by fungus and its elicitor were identified and it was revealed that most of them are involved in defense response against pathogens (Kim et al 2009). Furthermore, proteomics analysis using resistant line and susceptible line to M. oryzae identified 4 metabolism-associated proteins such as fructokinase, triosephosphate isomerase and 2 defense proteins, GST and aminopeptidase N, indicating that proteomics analysis is a useful approach for identifying novel proteins related in pathogen resistance (Ryu et al 2009). Meanwhile, this proteomics approach has been also used to investigate physiological interaction between plant and beneficial microorganisms promoting plant growth as well as plant response to pathogen.…”