Proteomic analysis of salt‐responsive proteins in oat roots (<i>Avena sativa</i> L.)

Bai, Jianhui; Liu, Jinghui; Wei-hong, Jiao; Sa, Rula; Zhang, Na; Jia, Ruizong

doi:10.1002/jsfa.7583

Cited by 14 publications

(10 citation statements)

References 45 publications

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“…Out of these transcripts CDS_23_Unigene_52 (acetylajmalan esterase-like); CDS_2064_Unigene_4074 (zinc-finger homeodomain 9-like) and CDS_243_Unigene_459 (vacuolar amino acid transporter 1) were random selections with high differences in the base mean values of control and stress samples, while CDS_17489_Unigene_28412 (UDP-glucuronic acid decarboxylase 2-like); CDS_26744_Unigene_43881 (cyclic nucleotide-gated ion channel 2-like); CDS_19742_Unigene_31773 (NRT1 PTR FAMILY–like) were salt stress responsive transcripts. Bai et al [68] reported that UDP-glucuronic acid decarboxylase was one of the differentially expressed proteins showing the reduced expression while performing the proteomic analysis of salt responsive proteins in oat roots and transcript (CDS_17489_Unigene_28412) from this study also shows down-regulation in both DGE and qRT PCR data. Jha et al [45] have demonstrated by their salinity stress experiments in Arabidopsis that cyclic nucleotide-gated ion channels (CNGCs) like CNGC1, CNGC19 and CNGC20 increase under salinity in roots, while transcript levels of CNGC19, CNGC3 and CNGC8 increased in shoots.…”

Section: Resultssupporting

confidence: 51%

Ocimum metabolomics in response to abiotic stresses: Cold, flood, drought and salinity

et al. 2019

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Ocimum tenuiflorum is a widely used medicinal plant since ancient times and still continues to be irreplaceable due to its properties. The plant has been explored chemically and pharmacologically, however, the molecular studies have been started lately. In an attempt to get a comprehensive overview of the abiotic stress response in O. tenuiflorum, de novo transcriptome sequencing of plant leaves under the cold, drought, flood and salinity stresses was carried out. A comparative differential gene expression (DGE) study was carried out between the common transcripts in each stress with respect to the control. KEGG pathway analysis and gene ontology (GO) enrichment studies exhibited several modifications in metabolic pathways as the result of four abiotic stresses. Besides this, a comparative metabolite profiling of stress and control samples was performed. Among the cold, drought, flood and salinity stresses, the plant was most susceptible to the cold stress. Severe treatments of all these abiotic stresses also decreased eugenol which is the main secondary metabolite present in the O. tenuiflorum plant. This investigation presents a comprehensive analysis of the abiotic stress effects in O. tenuiflorum. Current study provides an insight to the status of pathway genes’ expression that help synthesizing economically valuable phenylpropanoids and terpenoids related to the adaptation of the plant. This study identified several putative abiotic stress tolerant genes which can be utilized to either breed stress tolerant O. tenuiflorum through pyramiding or generating transgenic plants.

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Section: Resultssupporting

confidence: 51%

Ocimum metabolomics in response to abiotic stresses: Cold, flood, drought and salinity

et al. 2019

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“…Saline-alkali stress severely inhibits plant growth and even leads to plant death, with seed germination and seedling growth being the most sensitive stages affected ( Ibrahim, 2016 ). Roots are the main site of nutrient uptake and salinity perception ( Bai et al, 2016 ), which plays an important role in plant growth and development. Roots are in direct contact with soil, and are the first perceiver and responder of soil environmental stress; roots are more sensitive than shoots, responding to alkali stress ( Xu et al, 2013 ; Wang et al, 2021 ).…”

Section: Discussionmentioning

confidence: 99%

Metabolic and Physiological Changes in the Roots of Two Oat Cultivars in Response to Complex Saline-Alkali Stress

et al. 2022

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Saline-alkali stress is a major abiotic stress factor in agricultural productivity. Oat (Avena sativa L.) is a saline-alkali tolerant crop species. However, molecular mechanisms of saline-alkali tolerance in oats remain unclear. To understand the physiological and molecular mechanisms underlying seedling saline-alkali tolerance in oats, the phenotypic and metabolic responses of two oat cultivars, Baiyan7 (BY, tolerant cultivar) and Yizhangyan4 (YZY, sensitive cultivar), were characterized under saline-alkali stress conditions. Compared with YZY, BY showed better adaptability to saline-alkali stress. A total of 151 and 96 differential metabolites induced by saline-alkali stress were identified in roots of BY and YZY, respectively. More detailed analyses indicated that enhancements of energy metabolism and accumulations of organic acids were the active strategies of oat roots, in response to complex saline-alkali stress. The BY utilized sugars via sugar consumption more effectively, while amino acids strengthened metabolism and upregulated lignin and might be the positive responses of BY roots to saline-alkali stress, which led to a higher osmotic adjustment of solute concentrations and cell growth. The YZY mainly used soluble sugars and flavonoids combined with sugars to form glycosides, as osmotic regulatory substances or antioxidant substances, to cope with saline-alkali stress. The analyses of different metabolites of roots of tolerant and sensitive cultivars provided an important theoretical basis for understanding the mechanisms of saline-alkali tolerance and increased our knowledge of plant metabolism regulation under stress. Meanwhile, some related metabolites, such as proline, betaine, and p-coumaryl alcohol, can also be used as candidates for screening saline-alkali tolerant oat cultivars.

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“…Metabolic adaptation of plants exposed to different stress requires sophisticated metabolic reorganization of multiple metabolic pathways. Several cytoplasmic enzymes involved in defensive secondary metabolism in roots were found to be altered under various abiotic stress conditions ( Alvarez et al, 2014 ; Bai et al, 2016 ). In the current study, four proteins related to phenylpropanoid biosynthesis were induced by osmotic stress.…”

Section: Discussionmentioning

confidence: 99%

Physiological and Proteomic Responses of Contrasting Alfalfa (Medicago sativa L.) Varieties to PEG-Induced Osmotic Stress

2018

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Drought severely limits global plant distribution and agricultural production. Elucidating the physiological and molecular mechanisms governing alfalfa stress responses will contribute to the improvement of drought tolerance in leguminous crops. In this study, the physiological and proteomic responses of two alfalfa (Medicago sativa L.) varieties contrasting in drought tolerance, Longzhong (drought-tolerant) and Gannong No. 3 (drought-sensitive), were comparatively assayed when seedlings were exposed to -1.2 MPa polyethylene glycol (PEG-6000) treatments for 15 days. The results showed that the levels of proline, malondialdehyde (MDA), hydrogen peroxide (H2O2), hydroxyl free radical (OH•) and superoxide anion free radical (O2•-) in both varieties were significantly increased, while the root activity, the superoxide dismutase (SOD) and glutathione reductase (GR) activities, and the ratios of reduced/oxidized ascorbate (AsA/DHA) and reduced/oxidized glutathione (GSH/GSSG) were significantly decreased. The soluble protein and soluble sugar contents, the total antioxidant capability (T-AOC) and the activities of peroxidase (POD), catalase (CAT), and ascorbate peroxidase (APX) first increased and then decreased with the increase in treatment days. Under osmotic stress, Longzhong exhibited lower levels of MDA, H2O2, OH• and O2•- but higher levels of SOD, CAT, APX, T-AOC and ratios of AsA/DHA and GSH/GSSG compared with Gannong No.3. Using isobaric tags for relative and absolute quantification (iTRAQ), 142 differentially accumulated proteins (DAPs) were identified from two alfalfa varieties, including 52 proteins (34 up-regulated and 18 down-regulated) in Longzhong, 71 proteins (28 up-regulated and 43 down-regulated) in Gannong No. 3, and 19 proteins (13 up-regulated and 6 down-regulated) shared by both varieties. Most of these DAPs were involved in stress and defense, protein metabolism, transmembrane transport, signal transduction, as well as cell wall and cytoskeleton metabolism. In conclusion, the stronger drought-tolerance of Longzhong was attributed to its higher osmotic adjustment capacity, greater ability to orchestrate its enzymatic and non-enzymatic antioxidant systems and thus avoid great oxidative damage in comparison to Gannong No. 3. Moreover, the involvement of other pathways, including carbohydrate metabolism, ROS detoxification, secondary metabolism, protein processing, ion and water transport, signal transduction, and cell wall adjustment, are important mechanisms for conferring drought tolerance in alfalfa.

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Proteomic analysis of salt‐responsive proteins in oat roots (Avena sativa L.)

Cited by 14 publications

References 45 publications

Ocimum metabolomics in response to abiotic stresses: Cold, flood, drought and salinity

Ocimum metabolomics in response to abiotic stresses: Cold, flood, drought and salinity

Metabolic and Physiological Changes in the Roots of Two Oat Cultivars in Response to Complex Saline-Alkali Stress

Physiological and Proteomic Responses of Contrasting Alfalfa (Medicago sativa L.) Varieties to PEG-Induced Osmotic Stress

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