Proteomic analysis of selenium embryotoxicity in cultured postimplantation rat embryos

Usami, Makoto; Mitsunaga, Katsuyoshi; Nakazawa, Ken; Doi, Osamu

doi:10.1002/bdrb.20145

Cited by 16 publications

(15 citation statements)

References 41 publications

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“…Therefore, an important reason of the decline in body weight gain might be that selenite inhibited the protein synthesis and/or oxidize protein macromolecules which were much more needed in the developed body muscles, especially at the beginning of life. Usami et al (2008) investigated selenium embryotoxicity and found quantitative changes in proteins, growth inhibition and morphological abnormalities of cultured embryos.…”

Section: Resultsmentioning

confidence: 99%

Neurobehavioral changes in mice offspring induced by prenatal exposure to acute toxicity of sodium selenite

2011

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Selenium is an essential element with a narrow margin between beneficial and toxic effects. This study was aimed to determine the neurobehavioral changes resulted from the prenatal exposure of mice to high doses of sodium selenite during fetal and early postnatal development. Atomic absorption for monitoring the placental transfer of selenium to offspring was employed. The developmental observations as well as the behavioral tests, such as sensory motor reflexes, and learning and memory test in automatic reflex conditioner (shuttle box) (active avoidance responses) were applied. Adult mice was assigned into three groups: the first group was remained as a control group given phosphate buffered saline; the second and the third groups were orally administrated sodium selenite at doses of 1 mg/kg and 4 mg/kg of the diet, respectively started from the 7 th day to the end of the gestation period. Appearance of body hair and opening of eyes of the pups from treated mothers were delayed in a dose-dependent manner. The body weight gain came significantly lower than those of the control especially at the higher dose. Selenite also inhibited the sensory motor reflexes in all elements of acts and postures in a dose dependent manner. The active avoidance training-test indicated that selenite exposure was associated with learning impairment. Acetylcholine recorded a significant decrease in almost all the period of this study. By using atomic absorption, we found a significant high concentration of selenium in the brain, liver and kidney until the 40 th postnatal day, indicating active transfer of selenium from mothers to embryos.

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Section: Resultsmentioning

confidence: 99%

Neurobehavioral changes in mice offspring induced by prenatal exposure to acute toxicity of sodium selenite

2011

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“…It was noted, however, that the identified proteins included alpha-fetoprotein [standard spot numbers (SSPs 4818, 4831)], phosphorylated cofilin-1 (SSP 6001), and serum albumin (SSPs 4832, 5805). These three proteins have been considered candidate protein biomarkers that may be involved in embryotoxic mechanisms, as reported in our previous studies (Usami et al, 2014;Usami et al, 2009;Usami et al, 2008).…”

Section: Effects Of Vpa On Embryonic Protein Expressionmentioning

confidence: 80%

“…Day 10.5 embryos (plug day = day 0.5) of Wistar rats (Crlj: WI, Charles River Laboratories Japan, Inc., Kanagawa, Japan) were cultured for 24 hr using the roller bottle method (Usami et al, 2008). VPA (sodium salt, CAS 1069-66-5, Calbiochem, Merck KGaA, Darmstadt, Germany) was dissolved in Hank's balanced salt solution (0.1 mL) and added to the culture medium (4.9 mL) composed of 100% rat serum at concentrations of 0, 0.3, 0.6, and 1.2 mM.…”

Section: Embryo Culture and Vpa Treatmentmentioning

confidence: 99%

“…To date, we have examined protein expression changes in cultured embryos exposed to certain developmental toxicants, i.e., selenium, indium, and ethanol, and found candidate protein biomarkers that may be involved in embryotoxic mechanisms (Usami et al, 2008;Usami et al, 2009;Usami et al, 2014). It is, however, still important to accumulate proteomic analysis data of developmental toxicants.…”

Section: Introductionmentioning

confidence: 99%

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Proteomic analysis of valproic acid-induced embryotoxicity in cultured post-implantation rat embryos

Usami

Takamatsu

Kazama

et al. 2017

Fundam. Toxicol. Sci.

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-Protein expression changes were examined in day 10.5 rat embryos cultured for 24 hr in the presence of valproic acid (VPA), using two-dimensional electrophoresis and mass spectrometry. Exposure to VPA at an embryotoxic concentration of 1.2 mM resulted in quantitative changes in many embryonic protein spots (22 decreased and 29 increased). For the increased protein spots, 10 proteins were identified, including alpha-fetoprotein, phosphorylated cofilin-1, and serum albumin. These proteins are candidate protein biomarkers that may be involved in embryotoxic mechanisms.

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“…The gender of rat embryos can be determined by polymerase chain reaction (PCR) of a sequence in Sry, a et al, 1998), in DNA samples from the embryonic tissues, i.e., the embryo proper or yolk sac membrane. There are, however, no reported methods applicable to comprehensive proteomic analyses of early postimplantation rat embryos, where both embryo proper and yolk sac membrane are Sexing of postimplantation rat embryos in stored twodimensional electrophoresis (2-DE) samples by polymerase chain reaction (PCR) of an Sry sequencees (Usami et al, 2007a(Usami et al, , 2008.…”

Section: Introductionmentioning

confidence: 99%

Sexing of postimplantation rat embryos in stored twodimensional electrophoresis (2-DE) samples by polymerase chain reaction (PCR) of an Sry sequence

et al. 2009

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-Proteomic analysis of developmental toxicity by two-dimensional electrophoresis (2-DE) may detect gender-related toxic effects in embryos without visible gender characteristics. In the present study, we explored sexing of rat embryo stored in frozen 2-DE samples by polymerase chain reacSry). The embryo proper and yolk sac membrane at gestation day 11 from Wistar rats were used for stored embryonic 2-DE samples. The embryonic 2-DE samples were desalted and their total DNA was extracted. The Sry sequence in the embryos with the PCR product of Sry were determined as male, and those without the product were determined as female. It was concluded that stored embryonic 2-DE samples could be used for retrospective examination of gender-related effects in proteomic analysis of developmental toxicity.

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Proteomic analysis of selenium embryotoxicity in cultured postimplantation rat embryos

Cited by 16 publications

References 41 publications

Neurobehavioral changes in mice offspring induced by prenatal exposure to acute toxicity of sodium selenite

Neurobehavioral changes in mice offspring induced by prenatal exposure to acute toxicity of sodium selenite

Proteomic analysis of valproic acid-induced embryotoxicity in cultured post-implantation rat embryos

Sexing of postimplantation rat embryos in stored twodimensional electrophoresis (2-DE) samples by polymerase chain reaction (PCR) of an Sry sequence

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