Proteomic analysis of seminal plasma in adolescents with and without varicocele

Zylbersztejn, Daniel Suslik; Andreoni, Cássio; Giudice, P.T. Del; Spaine, D.M.; Borsari, Luciana; Souza, Gustavo H. M. F.; Bertolla, Ricardo Pimenta; Fraietta, Renato

doi:10.1016/j.fertnstert.2012.08.048

Cited by 69 publications

(52 citation statements)

References 35 publications

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“…Recently, there have been several studies about differences between protein profile in sperm or seminal plasma in men with and without varicocele [6,7,11]. The first report was ours so that we detected 15 significant differences in sperm protein expression [6].…”

Section: Discussionmentioning

confidence: 59%

Study of the effect of varicocelectomy on sperm proteins expression in patients with varicocele and poor sperm quality by using two-dimensional gel electrophoresis

Hosseinifar

Sabbaghian

Nasrabadi

et al. 2014

J Assist Reprod Genet

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Purpose To compare proteomic profiles of spermatozoa from patients with varicocele and poor sperm quality before and after varicocelectomy. Methods This work was designed as a prospective and observational study. The study was based on 20 men with varicocele grade 3 and poor sperm quality undergoing varicocelectomy at the Fertility Unit of Royan institute in 2009. Two semen samples were collected, one before varicocelectomy and the other after surgery. Protein separation was done by two-dimensional protein electrophoresis, and analyzed by gel densitometry and mass spectrometry. Differential sperm protein expression levels were measured by gel densitometry. Results Comparison of the sperm parameters showed that sperm motility and concentration were increased after varicocelectomy. At the level of protein, a total of 3 protein spots were identified whose expression was significantly lower in sperm samples before varicocelectomy compared with after surgery including heat shock protein A5 (HSPA5), superoxide dismutase 1 (SOD1) and δ-subunit of the catalytic core of mitochondrial adenosine triphosphate synthase (ATP5D). Conclusions High grade varicocoele affects sperm protein expression presumably because of increasing testicular temperature. These proteins play essential roles in sperm production, DNA integrity protection, and sperm motility. This novel study demonstrates that varicocelectomy can improve both sperm quality and proteins expression.

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Section: Discussionmentioning

confidence: 59%

Study of the effect of varicocelectomy on sperm proteins expression in patients with varicocele and poor sperm quality by using two-dimensional gel electrophoresis

Hosseinifar

Sabbaghian

Nasrabadi

et al. 2014

J Assist Reprod Genet

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show abstract

“…A very recent study[138] have reported proteomic profiles of seminal plasma from adolescent males presenting varicocele concluding that these proteins are potential markers for an early diagnosis of this disease. Indeed, Fu-Jun and Xiao-Fang[139] analyzed the proteome in order to find candidate proteins of sperm maturation and they found 270 proteins.…”

Section: Proteomics In Assisted Reproductionmentioning

confidence: 99%

OMICS: Current and future perspectives in reproductive medicine and technology

Egea¹,

Puchalt²,

Meseguer³

et al. 2014

J Hum Reprod Sci

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Many couples present fertility problems at their reproductive age, and although in the last years, the efficiency of assisted reproduction techniques has increased, these are still far from being 100% effective. A key issue in this field is the proper assessment of germ cells, embryos and endometrium quality, in order to determine the actual likelihood to succeed. Currently available analysis is mainly based on morphological features of oocytes, sperm and embryos and although these strategies have improved the results, there is an urgent need of new diagnostic and therapeutic tools. The emergence of the - OMICS technologies (epigenomics, genomics, transcriptomics, proteomics and metabolomics) permitted the improvement on the knowledge in this field, by providing with a huge amount of information regarding the biological processes involved in reproductive success, thereby getting a broader view of complex biological systems with a relatively low cost and effort.

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“…[111213141516] I.e. Pilch and Mann reported 923 proteins in human seminal plasma from healthy men by mass spectrometry (MS).…”

Section: Introductionmentioning

confidence: 99%

Differential protein expression in seminal plasma from fertile and infertile males

Alvarez

Markert

et al. 2014

J Hum Reprod Sci

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AIM:The aim of this study was to analyze human seminal plasma proteins in association with male fertility status using the proteomic mass spectrometry technology Surface-Enhanced Laser Desorption Ionization Time-of-Flight (SELDI-TOF-MS).MATERIALS AND METHODS:Semen analysis was performed using conventional methods. Protein profiles of the seminal plasma were obtained by SELDI-TOF mass spectrometry over a strong anion exchanger, ProteinChip® Q10 array.RESULTS AND CONCLUSION:We found statistically significant differences in motility and sperm count between fertile and infertile men. In addition, we observed ten seminal proteins that are significantly up-regulated in the infertile group. In conclusion, comparison of seminal plasma proteome in fertile and infertile men provides new aspects in the physiology of male fertility and might help in identifying novel markers of male infertility.

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Proteomic analysis of seminal plasma in adolescents with and without varicocele

Cited by 69 publications

References 35 publications

Study of the effect of varicocelectomy on sperm proteins expression in patients with varicocele and poor sperm quality by using two-dimensional gel electrophoresis

Study of the effect of varicocelectomy on sperm proteins expression in patients with varicocele and poor sperm quality by using two-dimensional gel electrophoresis

OMICS: Current and future perspectives in reproductive medicine and technology

Differential protein expression in seminal plasma from fertile and infertile males

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