Proteomic analysis of sensitive and multi drug resistant Mycobacterium tuberculosis strains

Abstract

Multidrug-resistant tuberculosis (MDR-TB) is caused by bacteria that are resistant to the most effective anti TB drugs (Isoniazid and Rifampicin) with or without resistance to other drugs. Novel intervention strategies to eliminate this disease based on finding proteins can be used for designing new drugs or new and reliable kits for diagnosis. The aim of this study was to compare the protein profile of MDR-TB with sensitive isolates. Two-dimensional gel electrophoresis (2DE) along with mass spectrometry is a powerful and effective tool to identification and characterization of Mycobacterium tuberculosis. Two-dimensional gel electrophoresis and matrix assisted laser desorption/ionization time-of-flight (MALDI-TOF) mass spectrometry was used for diagnosis and comparison of proteins. We identified 14 protein spots in MDR-TB isolates that 2DE analysis showed these spots absent in M. tuberculosis sensitive isolates (Rv1876, Rv0379, Rv0147, Rv2031c, Rv3597c, Rv1886c, MT0493, Rv0440, Rv3614c, Rv1626, Rv0443, Rv0475, Rv3057 and unknown protein. The results showed 22 protein spots which were up regulated (or expressed) by the MDR-TB isolates, ()). Two up regulated protein spots were identified in sensitive isolate (Rv1133c and Rv0685). These data will provide valuable clues in further investigation for suitable TB rapid tests or drug targets against drug resistant and sensitive of M. tuberculosis.

Monitoring of Bactericidal Effects of Silver Nanoparticles Based on Protein Signatures and VOC Emissions from Escherichia coli and Selected Salivary Bacteria

“…Effectiveness of function of AgNPs was investigated by means of various techniques including well and disc diffusion methods and flow cytometry [5,6,7,8,9]. In the current study, we employed matrix-assisted laser desorption/ionization–time-of-flight mass spectrometry (MALDI-TOF MS), which has emerged as a powerful technique for identification of microorganisms [10,11] and for investigation of, for instance, bacterial drug resistance [12]. Protein profiles obtained within the mass range of 2000 to 20,000 Da using MALDI-TOF MS can reflect many physiological states of bacteria [10].…”

Comparing mRNA expression and protein abundance in MDR Mycobacterium tuberculosis: Novel protein candidates, Rv0443, Rv0379 and Rv0147 as TB potential diagnostic or therapeutic targets

“…In addition, PPE10; a protein secreted by ESX-5, as the major protein responsible for capsular integrity, attenuates virulence in the early stages of infection and is thought to play a role in immune evasion [ 46 ]. Similarly, our previous study showed that these identified proteins were differentially expressed between MDR and susceptible TB and could be considered as T cell activators and may be candidate antigens for the development of a novel TB vaccine or therapeutic strategy against tuberculosis [ 47 ].…”

Immuno-proteomics analysis between OMV of vaccine and dominant wild type strains of Bordetella pertussis in Iran

“…After IEF, strips were equilibrated in 3 mL of equilibration buffer (150 mM Tris-HCl, pH 8.8, 6 M urea, 50% glycerol, 20% SDS, and bromophenol blue) with DTT (2%) for 15 min at RT, followed by 3 mL of equilibration buffer with iodoacetamide (2.5%) for 15 min at RT. The proteins were separated in second dimension on 10% SDS -PAGE in a vertical electrophoretic dual gel system and were visualized by Coomassie (R -250) staining method (13).…”