Proteomic analysis of striatal neuronal cell cultures after morphine administration

Bodzoń‐Kułakowska, Anna; Suder, Piotr; Mak, Paweł; Bierczyńska-Krzysik, Anna; Lubec, Gert; Walczak, Beata; Kotlińska, Jolanta; Silberring, Jerzy

doi:10.1002/jssc.200800464

Cited by 31 publications

(27 citation statements)

References 61 publications

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“…In an earlier review, we presented a detailed summary of proteins involved in these biological processes and discussed their potential roles in the physiological conditions of drug dependence [17]. The correlation of these biological processes with each addictive drug also has been discussed in some proteomics studies [11,28,34,43,46,52].…”

Section: Proteins Associated With the Exposure Of Multiple Addictive mentioning

confidence: 97%

“…Most of the published investigations are based on animal models or cell lines. Because of their importance in the development and maintenance of drug dependence, a few brain regions, such as the NAc [50], frontal cortex [12,51], hippocampus [12], and striatum [12,52], are the primary targets of proteomics analysis in the study of morphine, heroin, or butorphanol addiction. Whole brain [53] or components of the nervous system, such as the spinal cord [54], dorsal root ganglia [55], or cultured neuronal cells [56,57], also have been investigated.…”

Section: Morphine/heroin/butorphanolmentioning

confidence: 99%

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Genes and Pathways Co-associated with the Exposure to Multiple Drugs of Abuse, Including Alcohol, Amphetamine/Methamphetamine, Cocaine, Marijuana, Morphine, and/or Nicotine: a Review of Proteomics Analyses

Wang

Yuan

2011

Mol Neurobiol

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Drug addiction is a chronic neuronal disease. In recent years, proteomics technology has been widely used to assess the protein expression in the brain tissues of both animals and humans exposed to addictive drugs. Through this approach, a large number of proteins potentially involved in the etiology of drug addictions have been identified, which provide a valuable resource to study protein function, biochemical pathways, and networks related to the molecular mechanisms underlying drug dependence. In this article, we summarize the recent application of proteomics to profiling protein expression patterns in animal or human brain tissues after the administration of alcohol, amphetamine/methamphetamine, cocaine, marijuana, morphine/heroin/butorphanol, or nicotine. From available reports, we compiled a list of 497 proteins associated with exposure to one or more addictive drugs, with 160 being related to exposure to at least two abused drugs. A number of biochemical pathways and biological processes appear to be enriched among these proteins, including synaptic transmission and signaling pathways related to neuronal functions. The data included in this work provide a summary and extension of the proteomics studies on drug addiction. Furthermore, the proteins and biological processes highlighted here may provide valuable insight into the cellular activities and biological processes in neurons in the development of drug addiction.

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Section: Proteins Associated With the Exposure Of Multiple Addictive mentioning

confidence: 97%

Section: Morphine/heroin/butorphanolmentioning

confidence: 99%

Genes and Pathways Co-associated with the Exposure to Multiple Drugs of Abuse, Including Alcohol, Amphetamine/Methamphetamine, Cocaine, Marijuana, Morphine, and/or Nicotine: a Review of Proteomics Analyses

Wang

Yuan

2011

Mol Neurobiol

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“…2D-PAGE was performed following Bodzon-Kulakowska [32], but with some modifications. The first dimension was performed on 18 cm immobilized non-linear gradient strips at pH 5-8.…”

Section: D-pagementioning

confidence: 99%

Effects of chronic tramadol exposure on the zebrafish brain: A proteomic study

Zhuo

Huang

et al. 2012

Journal of Proteomics

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“…Briefly, peptides were loaded onto a reversed phase trapping column (0.3 mm ϫ 5.0 mm), and subsequently resolved on a capillary C 18 PepMap column (0.1 mm ϫ 150 mm, 3 m, 100 Å, Dionex). Peptides were eluted using a 70 min gradient of solvent A and solvent B as follows: 5% to 8% B from 0 -4 min, 18% B at 34 min, 35% B at 57 min, and 95% B at 64 min.…”

Section: Methodsmentioning

confidence: 99%

“…Proteomic analysis has been used to elucidate both the in vitro (18,19) and in vivo (20 -26) changes in the protein profiles of synapses that exhibit drug-induced alterations in response to opiate exposure. For example, the use of subcellular fractionation, which allows for the separation and generation of protein fractions selectively enriched in either pre-or postsynaptic proteins (25,27), in combination with proteomic analysis has revealed that an acute escalating dose of morphine administration produces significant alterations in the postsynaptic density (PSD) 1 associated protein network in the hippocampus.…”

mentioning

confidence: 99%

Morphine Regulated Synaptic Networks Revealed by Integrated Proteomics and Network Analysis

Stockton¹,

Gomes²,

Liu³

et al. 2015

Molecular & Cellular Proteomics

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Despite its efficacy, the use of morphine for the treatment of chronic pain remains limited because of the rapid development of tolerance, dependence and ultimately addiction. These undesired effects are thought to be because of alterations in synaptic transmission and neuroplasticity within the reward circuitry including the striatum. In this study we used subcellular fractionation and quantitative proteomics combined with computational approaches to investigate the morphine-induced protein profile changes at the striatal postsynaptic density. Over 2,600 proteins were identified by mass spectrometry analysis of subcellular fractions enriched in postsynaptic density associated proteins from saline or morphine-treated striata. Among these, the levels of 34 proteins were differentially altered in response to morphine. These include proteins involved in G-protein coupled receptor signaling, regulation of transcription and translation, chaperones, and protein degradation pathways. The altered expression levels of several of these proteins was validated by Western blotting analysis. Using Genes2Fans software suite we connected the differentially expressed proteins with proteins identified within the known background protein-protein interaction network. This led to the generation of a network consisting of 116 proteins with 40 significant intermediates. To validate this, we confirmed the presence of three proteins predicted to be significant intermediates: caspase-3, receptor-interacting serine/threonine protein kinase 3 and NEDD4 (an E3-ubiquitin ligase identified as a neural precursor cell expressed developmentally down-regulated protein 4). Because this morphine-regulated network predicted alterations in proteasomal degradation, we examined the global ubiquitination state of postsynaptic density proteins and found it to be substantially altered. Together, these findings suggest a role for protein degradation and for the ubiquitin/proteasomal system in the etiology of opiate dependence and addiction. Morphine and other opiates are the drugs of choice for the treatment of both severe and chronic pain. However, the utility of these compounds in the clinical setting is limited because of the rapid development of tolerance, physical dependence and addiction. The underlying cellular and molecular alterations through which chronic opiate exposure results in the persistent behavioral phenomenon of addiction remain poorly understood. However, there is evidence suggesting that the molecular composition of synapses within the reward circuitry of the central nervous system, particularly in the striatum, may be significantly altered (1). Moreover, given the critical involvement of the striatum in translating emotional or rewarding stimuli into motivated behaviors, alterations in this region induced by morphine and other abused drugs could contribute significantly to the pathophysiological responses at the heart of addiction (2). Although the debate surrounding the molecular and cellular mechanisms by which repeated drug adminis...

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Proteomic analysis of striatal neuronal cell cultures after morphine administration

Cited by 31 publications

References 61 publications

Genes and Pathways Co-associated with the Exposure to Multiple Drugs of Abuse, Including Alcohol, Amphetamine/Methamphetamine, Cocaine, Marijuana, Morphine, and/or Nicotine: a Review of Proteomics Analyses

Genes and Pathways Co-associated with the Exposure to Multiple Drugs of Abuse, Including Alcohol, Amphetamine/Methamphetamine, Cocaine, Marijuana, Morphine, and/or Nicotine: a Review of Proteomics Analyses

Effects of chronic tramadol exposure on the zebrafish brain: A proteomic study

Morphine Regulated Synaptic Networks Revealed by Integrated Proteomics and Network Analysis

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