Proteomic Analysis of the Antibacterial Mechanism of Action of Juglone against <i>Staphylococcus aureus</i>

Wang, Jiayi; Wang, Zixue; Wu, Rina; Jiang, Donghua; Bai, Bing; D, Tan; Yan, Tingcai; Sun, Xiyun; Zhang, Qi; Wu, Zhaoxia

doi:10.1177/1934578x1601100632

Cited by 11 publications

(12 citation statements)

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“…Most of these proteins involved in translation and suggested that inhibition of translation might be the common pathway in the antifungal effect. Similar results had been obtained in a previous study [ 37 ], which reported that Pseudomonas aeruginosa treated with AgNPs–GE induces the expression of energy metabolism proteins. These results indicate that C .…”

Section: Discussionsupporting

confidence: 91%

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Antifungal activity, main active components and mechanism of Curcuma longa extract against Fusarium graminearum

et al. 2018

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Curcuma longa possesses powerful antifungal activity, as demonstrated in many studies. In this study, the antifungal spectrum of Curcuma longa alcohol extract was determined, and the resulting EC50 values (mg/mL) of its extract on eleven fungi, including Fusarium graminearum, Fusarium chlamydosporum, Alternaria alternate, Fusarium tricinctum, Sclerotinia sclerotiorum, Botrytis cinerea, Fusarium culmorum, Rhizopus oryzae, Cladosporium cladosporioides, Fusarium oxysporum and Colletotrichum higginsianum, were 0.1088, 0.1742, 0.1888, 0.2547, 0.3135, 0.3825, 0.4229, 1.2086, 4.5176, 3.8833 and 5.0183, respectively. Among them, F. graminearum was selected to determine the inhibitory effects of the compounds (including curdione, isocurcumenol, curcumenol, curzerene, β-elemene, curcumin, germacrone and curcumol) derived from Curcuma longa. In addition, the antifungal activities of curdione, curcumenol, curzerene, curcumol and isocurcumenol and the synergies of the complexes of curdione and seven other chemicals were investigated. Differential proteomics of F. graminearum was also compared, and at least 2021 reproducible protein spots were identified. Among these spots, 46 were classified as differentially expressed proteins, and these proteins are involved in energy metabolism, tRNA synthesis and glucose metabolism. Furthermore, several fungal physiological differences were also analysed. The antifungal effect included fungal cell membrane disruption and inhibition of ergosterol synthesis, respiration, succinate dehydrogenase (SDH) and NADH oxidase.

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Section: Discussionsupporting

confidence: 91%

“…According to the soft guide, the spots were detected, matched and normalized based on the density of the gel and the percent volume. Calculations for the spots were performed at different statistical levels by the Kolmogorov-Smirnov test using ImageMaster 7.0 [ 35 – 37 ].…”

Section: Methodsmentioning

confidence: 99%

Antifungal activity, main active components and mechanism of Curcuma longa extract against Fusarium graminearum

et al. 2018

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“…Juglone has been shown to possess antibacterial activities [57,58], and reportedly inhibits Gram-positive bacteria, Staphylococcus aureus [98]. Juglone exhibits a selective antimicrobial activity against different sources of Staphylococcus aureus strains.…”

Section: Antimicrobial Activity Of Juglonementioning

confidence: 99%

“…It is also likely that the mechanisms of antifungal and antibacterial actions of juglone involve redox cycling [14]. A bioinformatics analysis has shown that bacterial proteins that participate in DNA, RNA and protein synthesis as well as in the tricarboxylic acid cycle are inhibited by juglone [98]. It has also been reported that the antifungal and antibacterial activities of juglone nanoparticles are higher than those of free juglone, indicating that the nanoparticle formulation may be a promising way to enhance the beneficial effects of juglone [100,101].…”

Section: Antimicrobial Activity Of Juglonementioning

confidence: 99%

Juglone in Oxidative Stress and Cell Signaling

2019

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Juglone (5-hydroxyl-1,4-naphthoquinone) is a phenolic compound found in walnuts. Because of the antioxidant capacities of phenolic compounds, juglone may serve to combat oxidative stress, thereby protecting against the development of various diseases and aging processes. However, being a quinone molecule, juglone could also act as a redox cycling agent and produce reactive oxygen species. Such prooxidant properties of juglone may confer health effects, such as by killing cancer cells. Further, recent studies revealed that juglone influences cell signaling. Notably, juglone is an inhibitor of Pin1 (peptidyl-prolyl cis/trans isomerase) that could regulate phosphorylation of Tau, implicating potential effects of juglone in Alzheimer’s disease. Juglone also activates mitogen-activated protein kinases that could promote cell survival, thereby protecting against conditions such as cardiac injury. This review describes recent advances in the understanding of the effects and roles of juglone in oxidative stress and cell signaling.

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“…The antibacterial mechanism of anthratectone inhibits cell wall and nucleic acid synthesis (Malmir et al, 2017). The antibacterial mechanism through inhibiting electron transport, decoupling phosphorylation oxidative, production of reactive oxygen species (ROS) in aerobic condition, inhibiting DNA and RNA synthesis were reported for naphtotectone and juglone compounds (Babula et al, 2009;Wang et al, 2016). Nayeem and Karvekar (2011) said the antibacterial mechanism through inhibiting hydrolytic enzymes or another interaction that activates microbial adhesion were reported for rutin, quercetin, gallic acid, ellagic acid, and sitosterol compounds.…”

Section: Isolation Methods and Work Mechanism Of Teak Leaf Antibacterimentioning

confidence: 99%

Mini-Review: Antibacterial Products from Teak Leaf Extract (Tectona grandis L.)

Azmi¹,

Ramadhanti²,

Hasibuan³

et al. 2020

ijrps

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Teak (Tectona grandis L.) is a plant that has a high resistance to weather changes and termite attacks. The dominant use of teak in the furniture industry left behind its leaf as agricultural waste (agro-waste). Several pharmacological activities have been identified in the teak leaf part. In this review article, the authors gathered information from previous studies regarding the potential use of teak leaf antibacterial compounds to be made into various antibacterial products, namely hand sanitizers, floor disinfectants, fruit and vegetable disinfectants, and deodorant. Literature searches are carried out through search engines and online journal databases. The results of the literature review show that there are at least eight antibacterial compounds from teak leaf that have been isolated, identified, and tested for their activity, namely anthratectone, naphthotectone, juglone, gallic acid, ellagic acid, rutin, quercetin, and sitosterol. Furthermore, it is known that the solvent that can be used for teak leaf extraction by providing the best antibacterial activity is ethanol. This information is used as a recommendation for optimization of teak leaf extraction as part of the author’s suggestion in making antibacterial products. In addition, previous research related to the use of ethanol extract from the teak leaf as an antibacterial product for liquid soap was also included. The study shows that the teak leaf extract liquid soap has promising activity compared to its comparable commercial soap, thereby strengthening the author's idea to utilize teak leaf extract as a component of the active ingredient of other antibacterial products, namely hand sanitizers, floor disinfectants, fruit and vegetable disinfectants, and deodorant.

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Proteomic Analysis of the Antibacterial Mechanism of Action of Juglone against Staphylococcus aureus

Cited by 11 publications

References 12 publications

Antifungal activity, main active components and mechanism of Curcuma longa extract against Fusarium graminearum

Antifungal activity, main active components and mechanism of Curcuma longa extract against Fusarium graminearum

Juglone in Oxidative Stress and Cell Signaling

Mini-Review: Antibacterial Products from Teak Leaf Extract (Tectona grandis L.)

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