Proteomic analysis of the Arabidopsis thaliana cell wall

Chivasa, Stephen; Ndimba, Bongani K.; Simon, William J.; Robertson, Duncan H. L.; Yu, Xiaolan; Knox, John; Bolwell, Paul; Slabas, Antoni R.

doi:10.1002/1522-2683(200206)23:11<1754::aid-elps1754>3.0.co;2-e

Cited by 223 publications

(217 citation statements)

References 49 publications

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“…Even though succinate, malate, and fumarate are part of the citric acid cycle in mitochondria and the glyoxylic acid cycle in peroxisomes, changes in the accumulation of malate, fumarate, and lactate in the apoplast could indicate a perturbation of malate dehydrogenase and lactate dehydrogenase (LDH) enzyme activities, which has long been known to be involved in providing NADH for H 2 O 2 production by peroxidases (Halliwell, 1977(Halliwell, , 1978. Interestingly, both malate dehydrogenase and LDH have been described as present in cell walls (Chivasa et al, 2002). Moreover, changes in the accumulation of small organic acids could also be due to a perturbation of cell metabolism.…”

Section: Knockdown Of Prx33/prx34 Leads To a Differential Accumulatiomentioning

confidence: 99%

A Peroxidase-Dependent Apoplastic Oxidative Burst in Cultured Arabidopsis Cells Functions in MAMP-Elicited Defense

O’Brien

Daudi²,

Finch³

et al. 2012

Plant Physiology

192

152

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Perception by plants of so-called microbe-associated molecular patterns (MAMPs) such as bacterial flagellin, referred to as pattern-triggered immunity, triggers a rapid transient accumulation of reactive oxygen species (ROS). We previously identified two cell wall peroxidases, PRX33 and PRX34, involved in apoplastic hydrogen peroxide (H 2 O 2 ) production in Arabidopsis (Arabidopsis thaliana). Here, we describe the generation of Arabidopsis tissue culture lines in which the expression of PRX33 and PRX34 is knocked down by antisense expression of a heterologous French bean (Phaseolus vulgaris) peroxidase cDNA construct. Using these tissue culture lines and two inhibitors of ROS generation, azide and diphenylene iodonium, we found that perxoxidases generate about half of the H 2 O 2 that accumulated in response to MAMP treatment and that NADPH oxidases and other sources such as mitochondria account for the remainder of the ROS. Knockdown of PRX33/PRX34 resulted in decreased expression of several MAMP-elicited genes, including MYB51, CYP79B2, and CYP81F2. Similarly, proteomic analysis showed that knockdown of PRX33/PRX34 led to the depletion of various MAMP-elicited defense-related proteins, including the two cysteine-rich peptides PDF2.2 and PDF2.3. Knockdown of PRX33/PRX34 also led to changes in the cell wall proteome, including increases in enzymes involved in cell wall remodeling, which may reflect enhanced cell wall expansion as a consequence of reduced H 2 O 2 -mediated cell wall cross-linking. Comparative metabolite profiling of a CaCl 2 extract of the PRX33/PRX34 knockdown lines showed significant changes in amino acids, aldehydes, and keto acids but not fatty acids and sugars. Overall, these data suggest that PRX33/PRX34-generated ROS production is involved in the orchestration of patterntriggered immunity in tissue culture cells.

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Section: Knockdown Of Prx33/prx34 Leads To a Differential Accumulatiomentioning

confidence: 99%

A Peroxidase-Dependent Apoplastic Oxidative Burst in Cultured Arabidopsis Cells Functions in MAMP-Elicited Defense

O’Brien

Daudi²,

Finch³

et al. 2012

Plant Physiology

192

152

View full text Add to dashboard Cite

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“…Such observed spotting patterns have been reported in previous studies [32,38,49,50]. Multiple spotting of the same protein in different locations on a 2-DE gel may be a reflection of protein isoforms due to PTMs or multigene families, products of proteolytic activities, the presence of multiple subunits of a single protein and/or the chemical modification of proteins during sample preparation [51].…”

Section: Mass Spectrometric Identification Of Salt Stress-regulated Smentioning

confidence: 99%

Identification and profiling of salinity stress-responsive proteins in Sorghum bicolor seedlings

Ngara

Ndimba

Borch-Jensen

et al. 2012

Journal of Proteomics

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Sorghum bicolor, a drought tolerant cereal crop, is not only an important food source in the semi arid/arid regions but also a potential model for studying and gaining a better understanding of the molecular mechanisms of drought and salt stress tolerance in cereals. In this study, seeds of a sweet sorghum variety, MN1618, were planted and grown on solid MS growth medium with or without 100 mM NaCl. Heat shock protein expression immunoblotting assays demonstrated that this salt treatment induced stress within natural physiological parameters for our experimental material. 2D PAGE in combination with MS/MS proteomics techniques were used to separate, visualise and identify salinity stress responsive proteins in young sorghum leaves. Out of 281 Coomassie stainable spots, 118 showed statistically significant responses (p<0.05) to salt stress treatments. Of the 118 spots, 79 were selected for tandem mass spectrometric identification, owing to their good resolution and abundance levels, and of these, 55 were positively identified. Identified proteins were divided into six functional categories including both known and novel/putative stress responsive proteins. Molecular and physiological functions of some of our proteins of interest are currently under investigation via bioinformatic and molecular biology approaches.

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“…Our identification of receptor kinases that are up-regulated in response to cell wall removal and regeneration suggests that these kinases play a key role in sensing cell wall perturbations. The activated kinases would in turn phosphorylate downstream signal transduction components and transcription factors, which would mediate cell wall biosynthesis/repair and stress signaling pathways (Baluska et al 2003;Chivasa et al 2002;Decreux and Messiaen 2005;Guo et al 2009a, b;Hematy and Hofte 2008;Hematy et al 2007;Kwon et al 2005;Lally et al 2001;Xu et al 2008).…”

Section: Proposed Model For Transcriptional Dynamics During Cell Wallmentioning

confidence: 99%

Transcriptional dynamics during cell wall removal and regeneration reveals key genes involved in cell wall development in rice

et al. 2011

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TitleTranscriptional dynamics during cell wall removal and regeneration reveals key genes involved in cell wall development in rice. Abstract Efficient and cost-effective conversion of plant biomass to usable forms of energy requires a thorough understanding of cell wall biosynthesis, modification and degradation. To elucidate these processes, we assessed the expression dynamics during enzymatic removal and regeneration of rice cell walls in suspension cells over time. In total, 928 genes exhibited significant up-regulation during cell wall removal, whereas, 79 genes were up-regulated during cell wall regeneration. Both gene sets are enriched for kinases, transcription factors and genes predicted to be involved in cell wall-related functions. Integration of the gene expression datasets with a catalog of known and/or predicted biochemical pathways from rice, revealed metabolic and hormonal pathways involved in cell wall degradation and regeneration. Rice lines carrying Tos17 mutations in genes up-regulated during cell wall removal exhibit dwarf phenotypes. Many of the genes up-regulated during cell wall development are also up-regulated in response to infection and environmental perturbations indicating a coordinated response to diverse types of stress.

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Proteomic analysis of the Arabidopsis thaliana cell wall

Cited by 223 publications

References 49 publications

A Peroxidase-Dependent Apoplastic Oxidative Burst in Cultured Arabidopsis Cells Functions in MAMP-Elicited Defense

A Peroxidase-Dependent Apoplastic Oxidative Burst in Cultured Arabidopsis Cells Functions in MAMP-Elicited Defense

Identification and profiling of salinity stress-responsive proteins in Sorghum bicolor seedlings

Transcriptional dynamics during cell wall removal and regeneration reveals key genes involved in cell wall development in rice

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