Proteomic analysis of the cytotoxic effects induced by the organogold(iii) complex Aubipy_cin cisplatin-resistant A2780 ovarian cancer cells: further evidence for the glycolytic pathway implication

Abstract

aThe cellular alterations produced in cisplatin-resistant A2780 ovarian cancer cells (A2780/R) upon treatment with the cytotoxic organogold(III) complex Aubipy c were investigated in depth through a classical proteomic approach. We observed that A2780/R cell exposure to a cytotoxic concentration of Aubipy c for 24 hours results in a conspicuous number of alterations at the protein level that were carefully examined. Notably, we observed that several affected proteins belong to the glucose metabolism system further supporting the idea that the cytotoxic effects of Aubipy c in A2780/R cells are mostly mediated by an impairment of glucose metabolism in excellent agreement with previous observations on the parent cisplatin-sensitive cell line.

Antiproliferative effects of two gold(I)-N-heterocyclic carbene complexes in A2780 human ovarian cancer cells: a comparative proteomic study

“…The cells were sonicated (15 s) and protein extracts were clarified by centrifugation at 8000g, 4°C for 15 min. Proteins were precipitated following a chloroform/methanol protocol [ 69 ] and the protein pellets were resolved in a buffer containing 8 M urea, 4% (w/v) 3-cholamidopropyl dimethylammonium-1-propane sulfonate (CHAPS), 65 mM dithioerythritol (DTE). The protein concentration was determined by the standard Bradford method (Bio-Rad Laboratories).…”

Current Progress and Perspectives on Using Gold Compounds for the Modulation of Tumor Cell Metabolism

“…They also studied the cytotoxicity of Aubipyc in cisplatin-resistant A2780 ovarian cancer cells. They found that this compound regulates glucose metabolism in tumor cells and significantly reduces lactate production through interaction with glycolytic enzymes such as aldolase C (ALDOC), LDHB, GAPDH, and PKM ( Gamberi et al, 2015 ). This gold (III) compound could therefore downregulate glucose metabolism in tumor cells.…”

Cancer cell-selective modulation of mitochondrial respiration and metabolism by potent organogold(iii) dithiocarbamates

“…[21][22][23] Both Au(I) and (III) complexes have gained considerable interest over the years as anticancer agents with preferential targeting of mitochondria, 24 thioredoxin proteasome, and inducing endoplasmic reticulum stress. [24][25][26][27][28][29][30][31][32] Gold complexes with multifaceted target mechanisms to evade resistance pathways are attractive.…”