Proteomic Analysis of the Effect of Extremely Low-Frequency Electromagnetic Fields (ELF-EMF) With Different Intensities in SH-SY5Y Neuroblastoma Cell Line

Rezaie-Tavirani, Mostafa; Hasanzadeh, Hadi; Seyyedi, Samaneh Sadat; Zali, Hakimeh

doi:10.15171/jlms.2017.14

Cited by 11 publications

(9 citation statements)

References 22 publications

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“…Rezaire-Taviraini et al, in their work, postulate that EMF with a frequency of 50 Hz affects the morphology of SH-SY5Y cells (neuroblastoma cell line), but without specifying any differences. In their work, researchers try to convince that the differences result from altered gene expression [ 10 ].…”

Section: Discussionmentioning

confidence: 99%

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The Influence of the Extremely Low Frequency Electromagnetic Field on Clear Cell Renal Carcinoma

Cios

Ciepielak

Stankiewicz

et al. 2021

IJMS

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The development of new technologies and industry is conducive to the increase in the number and variety of electromagnetic field (EMF) sources in our environment. The main sources of EMF are high-voltage lines, household appliances, audio/video devices, mobile phones, radio stations, and radar devices. In the growing use of electronic devices, scientists are increasingly interested in the effects of EMF on human health. Even though many studies on the effects of EMF have already been carried out, none of them has shown a significant effect on mammals, including humans. Moreover, it is not entirely clear how EMF influences cell behavior. The International Agency for Research on Cancer on 31 May 2011, classified PEM as a possible carcinogenic factor. This study aimed to investigate the effect of the electromagnetic field on morphological and functional changes in clear cell renal carcinoma. The research was carried out on in vitro cultures of four cell lines: HEK293, 786-O 769-P, and Caki1. The results of the research showed that the EMF of low frequency had a slight effect on the viability of cells. EMF, which induced cell arrest in the G1 phase, increased the number of early apoptotic cells and decreased the number of viable cells in the 786-O line. EMF did not affect the proliferation and viability of HEK293 cells. Extreme low-frequency EMF (ELF-EMF) also showed an inhibitory effect on the migration and metastatic properties of clear cell kidney cancer cells. Moreover, shortly after the end of ELF-EMF exposure, significant increases in ROS levels were observed in all tested cell lines. As part of the work, it was shown that low-frequency EMF shows an inhibitory effect on the proliferation of primary cancer cells, diminishing their migratory, invasive, and metastatic abilities. It also increases the apoptosis of cancer cells and the amount of reactive oxygen species. Based on the results of our research, we want to point up that the effect of ELF-EMF depends on a specific metabolic state or at a specific stage in the cell cycle of the cells under study.

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Section: Discussionmentioning

confidence: 99%

“…The reduced metastatic properties of tumor lines after EMF exposure are also confirmed by the results of the migration and invasion assays. This may be due to slight changes in the extracellular matrix, and not, as some scientists claim, the influence of EMF on the cytoskeleton of cancer cells [ 10 , 13 , 14 ].…”

Section: Discussionmentioning

confidence: 99%

The Influence of the Extremely Low Frequency Electromagnetic Field on Clear Cell Renal Carcinoma

Cios

Ciepielak

Stankiewicz

et al. 2021

IJMS

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“…These conditions determine the effect in the exposed samples. A proteomic study in SH-SY5Y cells exposed to 0.5 and 1 mT at 50 Hz for 3 h revealed that cell morphology, proliferation rate, and protein expression can be affected in a dose-dependent manner by an increased intensity of ELM-EMF exposure [Rezaie-Tavirani et al, 2017]. In addition, it has been demonstrated that pulsed EMFs can promote either proliferation or cell death, in an amplitude-, frequency-, and exposure-time-dependent manner [Akbarnejad et al, 2017].…”

Section: Discussionmentioning

confidence: 99%

An Extremely Low‐Frequency Vortex Magnetic Field Modifies Protein Expression, Rearranges the Cytoskeleton, and Induces Apoptosis of a Human Neuroblastoma Cell Line

Aparicio‐Bautista

Chávez-Valenzuela

Ambriz-Álvarez

et al. 2022

Bioelectromagnetics

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Homogeneous extremely low‐frequency electromagnetic fields (ELF‐EMFs) alter biological phenomena, including the cell phenotype and proliferation rate. Heterogenous vortex magnetic fields (VMFs), a new approach of exposure to magnetic fields, induce systematic movements on charged biomolecules from target cells; however, the effect of VMFs on living systems remains uncertain. Here, we designed, constructed, and characterized an ELF‐VMF‐modified Rodin's coil to expose SH‐SY5Y cells. Samples were analyzed by performing 2D‐differential‐gel electrophoresis, identified by MALDI‐TOF/TOF, validated by western blotting, and characterized by confocal microscopy. A total of 106 protein spots were differentially expressed; 40 spots were downregulated and 66 were upregulated in the exposed cell proteome, compared to the control cell proteome. The identified spots are associated with cytoskeleton and cell viability proteins, and according to the protein–protein interaction network, a significant interaction among them was found. Our data revealed a decrease in cell survival associated with apoptotic cells without effects on the cell cycle, as well as evident changes in the cytoskeleton. We demonstrated that ELF‐VMFs, at a specific frequency and exposure time, alter the cell proteome and structurally affect the target cells. This is the first report showing that VMF application might be a versatile system for testing different hypotheses in living systems, using appropriate exposure parameters.© 2022 Bioelectromagnetics Society.

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“…4 Additional information in this relationship could be achieved via the bioinformatic study of molecular data such as proteomics. 12 Moreover, bioinformatics is a useful method to study mechanism of laser therapy via analyzing the most central biomarkers in an interaction network. 8 Here, the proteomics data of IR laser treatment on myoblast (C2C12 cell line) of Murine 4 were employed for our bioinformatic assessments.…”

Section: Introductionmentioning

confidence: 99%

Muscle Recovery Is Highlighted by IR Laser Therapy

et al. 2019

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Introduction: In sports medicine, laser application has been well-established for the recovery of muscles. The mechanisms by which benefits of this kind of therapy can be studied is molecular research approach. Protein-protein interaction network analysis as one of the important complementary studies of proteomics can accelerate this goal by the identification of novel contributing markers. Methods: By the use of Cytoscape V3.7.1 and its applications, a network of differential expressed proteins (DEPs) from IR laser treatment samples were constructed and analyzed. Six hub-bottlenecks were determined, 4 of which were from differentially expressed proteins. Results: ClueGO discovered 4 biological processes related to these hub-bottlenecks that their function could alter due to IR laser therapy. Conclusion: In fact, by the expression changes of hub-bottlenecks including the up-regulation of HSP90s, one of the prominent biological processes in muscle recovery could be activated. This process is called nitric oxide synthase (NOS) activation that could be proposed as one of the underlying mechanisms of IR laser treatments in muscle recovery.

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Proteomic Analysis of the Effect of Extremely Low-Frequency Electromagnetic Fields (ELF-EMF) With Different Intensities in SH-SY5Y Neuroblastoma Cell Line

Cited by 11 publications

References 22 publications

The Influence of the Extremely Low Frequency Electromagnetic Field on Clear Cell Renal Carcinoma

The Influence of the Extremely Low Frequency Electromagnetic Field on Clear Cell Renal Carcinoma

An Extremely Low‐Frequency Vortex Magnetic Field Modifies Protein Expression, Rearranges the Cytoskeleton, and Induces Apoptosis of a Human Neuroblastoma Cell Line

Muscle Recovery Is Highlighted by IR Laser Therapy

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