Proteomic Analysis of the Hippocampus in Mouse Models of Trigeminal Neuralgia and Inescapable Shock-Induced Depression

Abstract

To investigate the behavioral and biomolecular similarity between neuralgia and depression, a trigeminal neuralgia (TN) mouse model was established by constriction of the infraorbital nerve (CION) to mimic clinical trigeminal neuropathic pain. A mouse learned helplessness (LH) model was developed to investigate inescapable foot-shock-induced psychiatric disorders like depression in humans. Mass spectrometry was used to assess changes in the biomolecules and signaling pathways in the hippocampus from TN or LH mice. TN mice developed not only significant mechanical allodynia but also depressive-like behaviors (mainly behavioral despair) at 2 weeks after CION, similar to LH mice. MS analysis demonstrated common and distinctive protein changes in the hippocampus between groups. Many protein function families (such as cell-to-cell signaling and interaction, and cell assembly and organization,) and signaling pathways (e.g., the Huntington's disease pathway) were involved in chronic neuralgia and depression. Together, these results demonstrated that the LH and TN models both develop depressive-like behaviors, and revealed the involvement of many psychiatric disorder-related biomolecules/pathways in the pathogenesis of TN and LH.

Effects of long non-coding RNA Gm14461 on pain transmission in trigeminal neuralgia

“…The mouse TN model was produced by CCI-ION via an intraoral approach as described previously [16]. Briefly, the animal was anesthetized with 4% sodium pentobarbital (50 mg/kg) by intraperitoneal injection.…”

Activation of ventrolateral orbital cortex improves mouse neuropathic pain–induced anxiodepression

“…The anxiodepressive-like behavioral tests were conducted from day 14 after CION, when the anxiodepressive-like behaviors occurred steadily according to our previous study (30). As shown in Figure 1, D-G, in the anxiety-related behavioral tests, the TN mice showed less time (1-way ANOVA, F 2,30 = 5.96, P = 0.007) and lower entries (1-way ANOVA, F 2,30 = 6.24, P = 0.005) in the open arm of the elevated plus maze (EPM), and they traveled less distance in the center arena of the open field (OF, 1-way ANOVA, F 2,30 = 8.07, P = 0.002).…”

“…The TN model was produced by chronic constriction injury of the unilateral infraorbital nerve via an intraoral approach, as described previously (30). Mice were anesthetized with sodium pentobarbital (50 mg/kg, i.p.…”

Melatonin Augments the Effects of Fluoxetine on Depression-Like Behavior and Hippocampal BDNF–TrkB Signaling

“…Depression is closely associated with abnormal BDNF-TrkB signaling [16,17] together with increased levels of circulating glucocorticoids [18,19] that can be reversed by antidepressants. Disrupted BDNF-TrkB signaling in the hippocampus and the prefrontal cortex has been implicated in the pathophysiology of depression [15,[20][21][22]. In addition, glucocorticoids are released upon stress challenge and regulate metabolic, immune, and neuronal responses [19].…”