Proteomic analysis of the nucleus accumbens of rats with different vulnerability to cocaine addiction

Castillo, Carmen; Morales, Lidia; Alguacil, Luis F.; Salas, Elisabet; Garrido, Elisa; Alonso, Elba; Pérez-Garcı́a, Carmen

doi:10.1016/j.neuropharm.2009.04.005

Cited by 34 publications

(35 citation statements)

References 53 publications

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“…In a proteomic study similar to ours, the ACO2 activator fumarate hydratase was one of the few proteins whose expression in the NAcc of rats that extinguish cocaine-induced CPP differed from that in the NAcc of rats that do not [7]. Indeed, it has been shown that a greater magnitude of CPP extinction correlates with increased tyrosine phosphorylation of ACO2 in the mouse prefrontal cortex [13], whereas lower levels of phosphorylation in the prefrontal cortex were evident after extinction of morphine-induced CPP [40].…”

Section: This May Be Due To the Fact That Translation Can Be Tightly supporting

confidence: 66%

“…We have shown previously that the expression of the ATP synthase F0 subunit 8 gene also differed between LEW and F344 in the NAcc [14]. In addition, the expression of subunit A, another subunit of this enzyme, also differs between rats depending on their ability to extinguish a previously established cocaine-induced CPP [7]. Another component of this complex, the mitochondrial precursor of the ATP synthase beta chain, was expressed more weakly in the NAcc after cocaine self-administration in monkeys [37].…”

Section: Protein Differences In the Amgmentioning

confidence: 95%

“…This protein seems to regulate some addiction-related phenomena and it has been shown that extinction of cocaine-induced conditioned place preference (CPP) by the growth factor midkine is correlated to the differential phosphorylation of PRDX6 in the dorsal striatum [13]. Interestingly, another member of the peroxiredoxin family, peroxiredoxin 2, was seen to be differentially regulated in the NAcc in response to a cocaine challenge when animals that do not extinguish cocaineinduced CPP were compared with those that do extinguish this behavior [7]. In addition, peroxiredoxin 2 has also been found to be decreased in the NAcc of rhesus monkeys following cocaine self-administration [37], in the NAcc of cocaine overdose victims [36] and in the striatum of rats after acute methamphetamine challenge [16].…”

Section: This May Be Due To the Fact That Translation Can Be Tightly mentioning

confidence: 99%

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Differential Protein Expression in the Nucleus Accumbens and Amygdala of Lewis and Fischer 344 Rats, and its Relevance in Drug Addiction

Roura-Martínez¹,

Ucha²,

Coria³

et al. 2014

Journal of Drug and Alcohol Research

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The Lewis (LEW) and Fischer 344 (F344) rat strains are widely used as models of genetic vulnerability to drug addiction, particularly since these two strains show different patterns of drug selfadministration. We previously performed an unbiased and genomewide microarray analysis to define the differences in gene expression in the nucleus accumbens (NAcc) and prefrontal cortex between LEW and F344 rats. In this study, we set out to determine the strain differences in the NAcc and amygdala at the protein level using bidimensional gel electrophoresis. As such, 11 proteins were found to be differentially expressed between these two strains, the activity of which was mainly related to vesicular trafficking (STXBP1 and GDI1), energy metabolism (ATP5B, ENO2, and ACOT7), and cytoskeletal formation. This information might be useful to search for new molecular targets to screen individuals at high risk of developing an addictive disorder.

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Section: This May Be Due To the Fact That Translation Can Be Tightly supporting

confidence: 66%

Section: Protein Differences In the Amgmentioning

confidence: 95%

Section: This May Be Due To the Fact That Translation Can Be Tightly mentioning

confidence: 99%

See 1 more Smart Citation

Differential Protein Expression in the Nucleus Accumbens and Amygdala of Lewis and Fischer 344 Rats, and its Relevance in Drug Addiction

Roura-Martínez¹,

Ucha²,

Coria³

et al. 2014

Journal of Drug and Alcohol Research

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“…In , del Castillo et al (2009 analyzed proteomes taken from the nucleus accumbens (NAc) of rats undergoing different rates of extinction following cocaineinduced place conditioning. Rats were divided into four main groups: (1) those that did not extinguish and were administered cocaine (NEC), (2) those that did not extinguish, but were administered saline (NE SAL), (3) those that did extinguish and were administered cocaine (EC), and (4) those that did extinguish, but were administered saline (E SAL).…”

Section: Proteomicsmentioning

confidence: 99%

Biomarkers for the Development of New Medications for Cocaine Dependence

Bough

Amur

Lao

et al. 2013

Neuropsychopharmacol

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There has been significant progress in personalized drug development. In large part, this has taken place in the oncology field and been due to the ability of researchers/clinicians to discover and develop novel drug development tools (DDTs), such as biomarkers. In cancer treatment research, biomarkers have permitted a more accurate pathophysiological characterization of an individual patient, and have enabled practitioners to target mechanistically the right drug, to the right patient, at the right time. Similar to cancer, patients with substance use disorders (SUDs) present clinically with heterogeneous symptomatology and respond variably to therapeutic interventions. If comparable biomarkers could be identified and developed for SUDs, significant diagnostic and therapeutic advances could be made. In this review, we highlight current opportunities and difficulties pertaining to the identification and development of biomarkers for SUDs. We focus on cocaine dependence as an example. Putative diagnostic, pharmacodynamic (PD), and predictive biomarkers for cocaine dependence are discussed across a range of methodological approaches. A possible cocaine-dependent clinical outcome assessment (COA)-another type of defined DDT-is also discussed. At present, biomarkers for cocaine dependence are in their infancy. Much additional research will be needed to identify, validate, and qualify these putative tools prior to their potential use for medications development and/or application to clinical practice. However, with a large unmet medical need and an estimated market size of several hundred million dollars per year, if developed, biomarkers for cocaine dependence will hold tremendous value to both industry and public health.

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“…Proteins involved in cytoskeleton structure, mitochondrial function, energy metabolism, and cell signaling were differentially expressed in this brain region. In another study, by examining the proteome in the NAc of Sprague Dawley rats, del Castillo et al [44] identified five proteins responsible for the different rate of extinction of cocaineinduced conditioned place preference. They also found that a single administration of cocaine could lead to an alteration of the expression of multiple proteins in this brain region.…”

Section: Cocainementioning

confidence: 99%

Genes and Pathways Co-associated with the Exposure to Multiple Drugs of Abuse, Including Alcohol, Amphetamine/Methamphetamine, Cocaine, Marijuana, Morphine, and/or Nicotine: a Review of Proteomics Analyses

Wang

Yuan

2011

Mol Neurobiol

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Drug addiction is a chronic neuronal disease. In recent years, proteomics technology has been widely used to assess the protein expression in the brain tissues of both animals and humans exposed to addictive drugs. Through this approach, a large number of proteins potentially involved in the etiology of drug addictions have been identified, which provide a valuable resource to study protein function, biochemical pathways, and networks related to the molecular mechanisms underlying drug dependence. In this article, we summarize the recent application of proteomics to profiling protein expression patterns in animal or human brain tissues after the administration of alcohol, amphetamine/methamphetamine, cocaine, marijuana, morphine/heroin/butorphanol, or nicotine. From available reports, we compiled a list of 497 proteins associated with exposure to one or more addictive drugs, with 160 being related to exposure to at least two abused drugs. A number of biochemical pathways and biological processes appear to be enriched among these proteins, including synaptic transmission and signaling pathways related to neuronal functions. The data included in this work provide a summary and extension of the proteomics studies on drug addiction. Furthermore, the proteins and biological processes highlighted here may provide valuable insight into the cellular activities and biological processes in neurons in the development of drug addiction.

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Proteomic analysis of the nucleus accumbens of rats with different vulnerability to cocaine addiction

Cited by 34 publications

References 53 publications

Differential Protein Expression in the Nucleus Accumbens and Amygdala of Lewis and Fischer 344 Rats, and its Relevance in Drug Addiction

Differential Protein Expression in the Nucleus Accumbens and Amygdala of Lewis and Fischer 344 Rats, and its Relevance in Drug Addiction

Biomarkers for the Development of New Medications for Cocaine Dependence

Genes and Pathways Co-associated with the Exposure to Multiple Drugs of Abuse, Including Alcohol, Amphetamine/Methamphetamine, Cocaine, Marijuana, Morphine, and/or Nicotine: a Review of Proteomics Analyses

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