Proteomic analysis of the occlusion-derived virus of Clostera anachoreta granulovirus

Abstract

To date, proteomic studies have been performed on occlusion-derived viruses (ODVs) from five members of the family Baculoviridae, genus Alphabaculovirus, but only a single member of the genus Betabaculovirus (Pieris rapae granulovirus). In this study, LC-MS/MS was used to analyse the ODV proteins of Clostera anachoreta granulovirus (ClanGV), another member of the genus Betabaculovirus. The results indicated that 73 proteins, including the products of 27 baculovirus core genes, were present in ClanGV ODVs. This is the largest number of ODV proteins identified in baculoviruses to date. To the best of our knowledge, 24 of these proteins were newly identified as ODV-associated proteins. Twelve of the proteins were shared by all seven of the other baculoviruses that have been analysed by proteomic techniques, including P49, PIF-2, ODV-EC43, P74, P6.9, P33, VP39, ODV-EC27, VP91, GP41, VLF-1 and VP1054. ClanGV shared between 20 and 36 ODV proteins with each of the other six baculoviruses that have been analysed by proteomics. Ten proteins were identified only as ODV components of ClanGV and PrGV: Clan22, Clan27, Clan69, Clan83, Clan84, Clan90, Clan116, Clan94, FGF-3 and ME53, the first seven of which were encoded by betabaculovirus-specific genes. These findings may provide novel insights into baculovirus structure as well as reveal similarities and differences between alphabaculoviruses and betabaculoviruses.

Induction of interferon-λ contributes to TLR3 and RIG-I activation-mediated inhibition of herpes simplex virus type 2 replication in human cervical epithelial cells

“…HSV-2 is among the most common human infectious viral pathogens, causing lifelong diseases with clinical manifestations including cold sores, genital lesions, corneal blindness and encephalitis (Fife et al, 2008;Sperling et al, 2008;Zhang et al, 2009Zhang et al, , 2012Dasgupta and Ben-Mohamed, 2011). Although diseases caused by HSV-2 are generally selflimiting, the virus can cause devastating dissimilated infections and encephalitis in immunocompromised individuals, such as newborns, persons with transplantation, and AIDS-patients (Zhang et al, 2015). Furthermore, HSV-2 infection promotes HIV infection and transmission (Shao et al, 2015;Wen et al, 2015).…”

Protein composition of the occlusion bodies of Epinotia aporema granulovirus

“…A database comprising all the ODV proteins detected in baculoviruses was generated using previous proteomic data sets [15][16][17][18][19][20][21][22]. The software BLASTP [24] and HHMER [25] were used to identify groups of orthologous proteins (orthogroups) among different proteomes by reciprocal best hits.…”

“…Additionally, Epap126 is shared between group II alphabaculoviruses and betabaculoviruses (except for ClanGV) (S1 Table). ODVs have been interrogated using MS-based techniques [20,21].…”

“…Virion proteomics has been applied to study several DNA virus families (Ascoviridae [10], Herpesviridae [11], Iridoviridae [12], Nudiviridae [13] and Poxviridae [14]). In relation with the present study, eight ODV proteomes of baculoviruses have been analyzed (AcMNPV [15], AgMNPV [16], ChchNPV [17], HearNPV [18], MabrNPV [19], ClanGV [20], PiraGV [21] and CuniNPV [22]). These datasets point at a complex virion comprising a large number of proteins involved in virion morphogenesis, OBs formation and infection of insect midgut epithelial cells.…”

Protein composition analysis of polyhedra matrix of Bombyx mori nucleopolyhedrovirus (BmNPV) showed powerful capacity of polyhedra to encapsulate foreign proteins

“…The availability of genome sequences facilitates component analyses of baculoviruses on a proteomic scale. To date, the ODV components of eight baculoviruses, i.e., Autographa californica multiple nucleopolyhedrovirus (AcMNPV) 12 , Culex nigripalpus nucleopolyhedrovirus (CuniNPV) 13 , BmNPV 14 , Chrysodeixis chalcites nucleopolyhedrovirus (ChchNPV) 15 , Helicoverpa armigera nucleopolyhedrovirus (HaNPV) 16 , 17 , Anticarsia gemmatalis multiple nucleopolyhedrovirus (AgMNPV) 18 , Mamestra brassicae nucleopolyhedrovirus (MabrNPV) 19 , Pieris rapae granulovirus (PrGV) 20 and Clostera anachoreta granulovirus (ClanGV) 21 , and the BV components of four viruses, i.e., AcMNPV 22 , HaNPV 17 , AgMNPV 18 and MabrNPV 19 , have been reported. These data shed light on the structure and assembly of baculovirus virions; however, in comparison, little is known regarding the structural composition of the polyhedra matrix.…”