Proteomic analysis of the renal effects of simulated occupational jet fuel exposure

Witzmann, Frank A.; Bauer, Mark D.; Fieno, Angela M.; Grant, Raymond A.; Keough, T.; Lacey, Martin P.; Sun, Yiping; Witten, Mark L.; Young, Renée

doi:10.1002/(sici)1522-2683(20000301)21:5<976::aid-elps976>3.0.co;2-5

Cited by 36 publications

(9 citation statements)

References 46 publications

(31 reference statements)

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“…These findings could lead to markers of lead toxicity which could aid in the diagnosis of this common but underdiagnosed disease. Several other studies have used 2DE to identify changes in renal protein expression caused by toxins including perfluoro-n-octanoic acid and perfluoro-n-decanoic acid (136), jet fuel (131,132), and fluoride (137). These studies demonstrate the potential for proteomic analysis to improve an understanding of the mechanisms of toxicity and to identify potential tissue biomarkers.…”

Section: Effect Of Toxins On Kidney Protein Expressionmentioning

confidence: 92%

Proteomics in renal research

Janech¹,

Raymond²,

Arthur³

2007

American Journal of Physiology-Renal Physiology

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Proteomic technologies are used with increasing frequency in the renal community. In this review, we highlight the use in renal research of a number of available techniques including two-dimensional gel electrophoresis, liquid chromatography/mass spectrometry, surface-enhanced laser desorption/ionization, capillary electrophoresis/mass spectrometry, and antibody and tissue arrays. These techniques have been used to identify proteins or changes in proteins specific to regions of the kidney or associated with renal diseases or toxicity. They have also been used to examine protein expression changes and posttranslational modifications of proteins during signaling. A number of studies have used proteomic methodologies to look for diagnostic biomarkers in body fluids. The rapid rate of development of the technologies along with the combination of classic physiological and biochemical techniques with proteomics will enable new discoveries.

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Section: Effect Of Toxins On Kidney Protein Expressionmentioning

confidence: 92%

Proteomics in renal research

Janech¹,

Raymond²,

Arthur³

2007

American Journal of Physiology-Renal Physiology

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“…Previous collaborative studies have analyzed the effect of high level JP-8 exposure (250, 1000, and 2500 mg/m 3 ) [2][3][4]. Significant quantitative differences in lung protein expression were found as a result of JP-8 exposure.…”

Section: Jp-8 Effects On Pulmonary Cells In Vivomentioning

confidence: 99%

Biomolecular Profiling of Jet Fuel Toxicity Using Proteomics

Witzmann¹

2006

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“…Several previously published studies have used 2-D gel based proteomic assay methods to evaluate JP-8 toxicity in mice or rats. Witzmann et al [25] analyzed kidney tissue from male Swiss-Webster mice exposed (nose-only) to 1000 mg/m 3 JP-8 in aerosol phase for 1 h/d for 5 d, then sacrificed 48 h post-exposure. JP-8 exposure significantly altered the abundance of 56 proteins (6% of proteins tested).…”

Section: Introductionmentioning

confidence: 99%

Analysis of rat testicular protein expression following 91‐day exposure to JP‐8 jet fuel vapor

Witzmann

Bobb

Briggs³

et al. 2003

Proteomics

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We analyzed protein expression in preparations from whole testis in adult male Sprague-Dawley rats exposed for 6 h/d for 91 consecutive days to jet propulsion fuel-8 (JP-8) in the vapor phase (0, 250, 500, or 1000 mg/m(3) +/- 10%), simulating a range of possible human occupational exposures. Whole body inhalation exposures were carefully controlled to eliminate aerosol phase, and subjects were sacrificed within 48 h postexposure. Organ fractions were solubilized and separated via large-scale, high resolution two-dimensional electrophoresis, and gel patterns scanned, digitized and processed for statistical analysis. Seventy-six different testis proteins were significantly increased or decreased in abundance in vapor-exposed groups, compared to controls, and dose-response profiles were often nonlinear. A number of the proteins were identified by peptide mass fingerprinting and related to histopathological or physiological deficits shown in previously published studies to occur with repeated exposure to hydrocarbon fuels or solvents. These results demonstrate a significant effect of JP-8 exposure on protein expression, particularly in protein expression in the rodent testis, and suggest that a 91 d exposure to jet fuel vapor induces changes of equal or greater magnitude to those reported previously for shorter duration JP-8 aerosol exposures.

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Proteomic analysis of the renal effects of simulated occupational jet fuel exposure

Cited by 36 publications

References 46 publications

Proteomics in renal research

Proteomics in renal research

Biomolecular Profiling of Jet Fuel Toxicity Using Proteomics

Analysis of rat testicular protein expression following 91‐day exposure to JP‐8 jet fuel vapor

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