Proteomic analysis of the urine of Dirofilaria immitis infected dogs

Hormaeche, Marta; Carretón, Elena; González‐Miguel, Javier; Gussoni, Stefania; Montoya-Alonso, José Alberto; Simón, F.; Morchón, Rodrigo

doi:10.1016/j.vetpar.2014.01.025

Cited by 19 publications

(13 citation statements)

References 8 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…All these anatomical changes at the vascular level leads to luminal obstruction and infarction, decrease of the blood flow, hypoxia, chronic pulmonary edema and hypertension and congestive heart failure [8, 9]. Moreover, the presence and death of microfilariae in renal capillaries is associated with inflammatory processes that damage the renal function [10]. Like other filarial species, D. immitis harbors intracellular symbiont bacteria of the genus Wolbachia [11] whose contribution to inflammatory processes is key [7, 12].…”

Section: Introductionmentioning

confidence: 99%

Angiogenic response in an in vitro model of dog microvascular endothelial cells stimulated with antigenic extracts from Dirofilaria immitis adult worms

Zueva

Morchón

Carretón³

et al. 2019

Parasites Vectors

Self Cite

View full text Add to dashboard Cite

Background Angiogenesis can occur under pathological conditions when stimuli such as inflammation, vascular obstruction or hypoxia exist. These stimuli are present in cardiopulmonary dirofilariosis ( Dirofilaria immitis ). The aim of this study was to analyze the capacity of D. immitis antigens to modify the expression of angiogenic factors and trigger the formation of pseudocapillaries (tube-like structures) in an in vitro model of endothelial cells. Methods The expression of VEGF-A, sFlt, mEndoglin and sEndoglin in cultures of canine microvascular endothelial cells stimulated with extract of adult worms of D. immitis obtained from an untreated dog (DiSA) and from a dog treated for 15 days with doxycycline (tDiSA), was determined by using commercial kits. The capacity of pseudocapillary formation was evaluated analyzing cell connections and cell groups in Matrigel cell cultures stimulated with DiSA and tDiSA. In both cases non-stimulated cultures were used as controls. Results First, we demonstrated that worms obtained from the dog treated with doxycycline showed a significantly lower amount of Wolbachia (less than 60%) than worms removed from the untreated dog. Only DiSA was able to significantly increase the expression of the proangiogenic factor VEGF-A in the endotelial cells cultures. None of the D. immitis extracts modified the expression of sFlt. tDiSA extract was able to modify the expression of the endoglins, significantly decreasing the expression of the pro-angiogenic mEndoglin and increasing the anti-angiogenic sEndoglin. The formation of pseudocapillaries was negatively influenced by tDiSA, which reduced the organization and number of cellular connections. Conclusions The ability of antigens from adult D. immitis worms to modify the expression of pro and anti-angiogenic factors in endotelial cell cultures was demonstrated, as well as the trend to form pseudocapillaries in vitro . The capacity of stimulation may be linked to the amount of Wolbachia present in the antigenic extracts.

show abstract

Section: Introductionmentioning

confidence: 99%

Angiogenic response in an in vitro model of dog microvascular endothelial cells stimulated with antigenic extracts from Dirofilaria immitis adult worms

Zueva

Morchón

Carretón³

et al. 2019

Parasites Vectors

Self Cite

View full text Add to dashboard Cite

show abstract

“…Previous studies suggested that Apo-A1is responsible of cholesterol transport from tissues to the liver [11] . However vena cava caudalis pressure overload as a result of decreased venous return to the heart may play a role on liverischemia-induced loss of free cholesterol esterification.…”

Section: Discussionmentioning

confidence: 99%

“…However vena cava caudalis pressure overload as a result of decreased venous return to the heart may play a role on liverischemia-induced loss of free cholesterol esterification. In this pathophysiology, high blood cholesterol plays a role on erythrolysis and hemoglobin release [11] . The mild increase in hemoglobin subunit ß protein detected in the present study might have resulted from increased demand of O 2 in the body in response to decreased cardiac output (lower aortic flow velocity), the myocardial contractility loss (lower FS), and decreased venous return (Jugular distention and higher pulmonary artery flow velocity) in dogs with DCM.…”

Section: Discussionmentioning

confidence: 99%

“…Samples and study designs are discussed due to the limitations of mass spectrometry [9] . In veterinary medicine proteomics were performed in such conditions as canine lymphoma [10] and heartworm disease [11] while canine DCM has not yet been studied using proteomics. In addition, although proteomics were performed in several canine body fluids such as cerebrospinal fluid [12] , urine [13] , bronchoalveolar lavage fluid [14] and follicular fluid [15] , no data have yet been presented on ascitic fluid in dogs with DCM.…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Dilate Kardiyomiyopatili Köpeklerde Serum ve Asites Sıvısı Proteomlarının Araştırılması

Kocatürk¹,

Baykal²,

Turkseven³

et al. 2015

Kafkas Univ Vet Fak Derg

View full text Add to dashboard Cite

The aim of the study was to investigate serum global proteomes in dogs with overt dilated cardiomyopathy (DCM) and to evaluate protein expression in serum with that in ascitic fluid. Eight healthy dogs (control group) and 8 dogs with DCM were included in the study. DCM was diagnosed based on echocardiographic evidence including increased left ventricular dimension at diastole and systole, increased E point to septal separation, and decreased fractional shortening. Serum and ascitic fluid samples were analyzed for proteomes using a label-free LC-MS/MS method. Proteome analyses revealed significantly different expressions of eight proteins in all samples. Expressions in serum of apolipoprotein (Apo) A1, Ig heavy chain V, superoxide dismutase and plasminogen were higher (P<0.001), while expressions of clusterin, hemoglobin subunit ß, Apo-CII, and β2 glycoprotein I (ß2GPI) were lower (P<0.001) in dogs with DCM than in control dogs. In addition, Apo-A1, clusterin, hemoglobin subunit ß, Ig heavy chain V, plasminogen and ß2GPI were down-regulated whereas Apo-CII and superoxide dismutase were up-regulated in ascitic fluid compared with serum in dogs with DCM. Data obtained in the present study suggest that serum and/or ascitic fluid proteomes may explain some of the pathophysiological mechanisms involved in the progression of DCM. Keywords: Dilated cardiomyopathy, DCM, Congestive heart failure, Proteomics, Dog Dilate Kardiyomiyopatili Köpeklerde Serum ve Asites Sıvısı Proteomlarının Araştırılması ÖzetÇalışmamızın amacı ileri düzeyde dilate kardiyomiyopati (DCM) tanısı konulan köpeklerde serum global proteomların araştırılması ve aynı hastaların asites sıvısı proteomları ile ilişkilerinin değerlendirilmesidir. Sekiz sağlıklı (kontrol grubu) ve 8 DCM'li köpek çalışmaya dahil edildi. DCM tanımlaması ekokardiografik olarak sistol ve diyastolde artmış sol ventriküler çap, artmış E point to septal separasyon değeri ve azalmış fraksiyonel kasılma verileri temelinde yapıldı. Serum ve asites sıvı örnekleri label-free LC-MS/MS metoduna göre analiz edilmiştir. Proteom analizi ile tüm örneklerde toplam sekiz adet proteom ekspresyonu belirlendi. DCM'li köpeklerde kontrol grubuna göre serum apolipoprotein (Apo) A1, Ig heavy chain V, superoksit dizmutaz ve plazminojen ekspresyonlarında artış (P<0.001), klasterin, hemoglobin subunit ß, Apo-CII, ve β2 glikoprotein I (ß2GPI) ekspresyonlarında ise azalma (p<0.001) belirlendi. Buna ek olarak; asites sıvısı serum örnekleri ile karşılaştırıldığında Apo-A1, klasterin, hemoglobin subunit ß, Ig heavy chain V, plazminojen ve ß2GPI azalırken, Apo-CII ve superoksit dizmutaz artış gösterdi. Çalışmada elde edilen bu verilerin; serum ve/veya asites sıvısı proteomlarının DCM gelişiminde rolü alan bazı patofizyolojik mekanizmaların açıklanmasına katkı verebileceği kanısındayız.

show abstract

“…This pathology leads to the appearance of blood vessel obstructions, infarction, blood flow decrease and hypoxia, as well as renal damage caused by microfilariae (Hormaeche et al ., 2014). Inflammation, hypoxia and tissue injury provide the required conditions to produce angiogenesis – the process to create new vessels from the pre-existing vasculature (Dennis et al ., 2011; Núñez-Gómez et al ., 2017).…”

Section: Introductionmentioning

confidence: 99%

Angiogenesis in cardiopulmonary dirofilariosis: does the Wolbachia surface protein have a pro- or anti-angiogenic effect?

et al. 2020

View full text Add to dashboard Cite

Cardiopulmonary dirofilariosis caused by Dirofilaria immitis produces inflammation, blood vessel obstruction and hypoxia, which are required conditions for the beginning of the process of neovascularization. Since D. immitis harbours intracellular symbiotic Wolbachia bacterium, the global understanding of the angiogenic process requires the analysis of the effect of the parasite molecules, but also that of Wolbachia. Canine primary lung microvascular endothelial cells were treated with the recombinant Wolbachia surface protein (rWSP) and the expression of angiogenic factors like Vascular Endothelial Growth Factor-A (VEGF-A), sFlt, membrane Endoglin (mEndoglin) and soluble Endoglin (sEndoglin), as well as the in vitro formation of pseudocapillaries, were measured. The analyses showed a significant increase in the expression of pro-angiogenic VEGF-A and anti-angiogenic sEndoglin, together with a significant decrease in both pro-angiogenic mEndoglin and pseudocapillary formation, compared to untreated controls. Due to the complexity of the angiogenic process and its relationship with other physiological processes like inflammation and fibrinolysis, these results might suggest that rWSP participate in various mechanisms related to each other and its effects might depend either on the balance between them or on the moment of their occurrence.

show abstract

Proteomic analysis of the urine of Dirofilaria immitis infected dogs

Cited by 19 publications

References 8 publications

Angiogenic response in an in vitro model of dog microvascular endothelial cells stimulated with antigenic extracts from Dirofilaria immitis adult worms

Angiogenic response in an in vitro model of dog microvascular endothelial cells stimulated with antigenic extracts from Dirofilaria immitis adult worms

Dilate Kardiyomiyopatili Köpeklerde Serum ve Asites Sıvısı Proteomlarının Araştırılması

Angiogenesis in cardiopulmonary dirofilariosis: does the Wolbachia surface protein have a pro- or anti-angiogenic effect?

Contact Info

Product

Resources

About