Proteomic analysis of Tn-bearing glycoproteins from different stages of melanoma cells reveals new biomarkers

Hoja-Łukowicz, Dorota; Szwed, Sabina; Laidler, Piotr; Lityń́ska, Anna

doi:10.1016/j.biochi.2018.05.010

Cited by 7 publications

(8 citation statements)

References 90 publications

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“…It has been well documented that Tn antigen is one of the most expressed tumour antigen found in many human cancers, such as colon, breast, cervix, stomach and skin . Many studies have established an association of Tn antigen expression with cancer progression and metastasis and poor outcome .…”

Section: Discussionmentioning

confidence: 99%

“…We showed that Tn antigen contributed to the increased invasive and metastatic properties of CRC in vitro and in vivo and revealed that up-regulation of H-Ras expression and subsequent activation of EMT could be a primary mechanism It has been well documented that Tn antigen is one of the most expressed tumour antigen found in many human cancers, such as colon, 27 breast, 28 cervix, 29 stomach 30 and skin. 31 Many studies have established an association of Tn antigen expression with cancer progression and metastasis and poor outcome. 13,32,33 However, it is still unclear whether Tn antigen plays a causative role in cancer progression and metastasis.…”

Section: Discussionmentioning

confidence: 99%

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Tn antigen promotes human colorectal cancer metastasis via H‐Ras mediated epithelial‐mesenchymal transition activation

Liu

Dong

et al. 2019

J Cellular Molecular Medi

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Tn antigen is a truncated O‐glycan, frequently detected in colorectal cancer ( CRC ), but its precise role in CRC metastasis is not well addressed. Here we investigated the effects of Core 1 β3Gal‐T specific molecular chaperone (Cosmc) deletion‐mediated Tn antigen exposure on CRC metastasis and its underlying mechanism. We first used CRISPR /Cas9 technology to knockout Cosmc, which is required for normal O‐glycosylation, and thereby obtained Tn‐positive CRC cells. We then investigated the biological consequences of Tn antigen expression in CRC . The results showed that Tn‐positive cells exhibited an enhanced metastatic capability both in vitro and in vivo. A further analysis indicated that Tn antigen expression induced typical activation of epithelial‐mesenchymal transition ( EMT ). Mechanistically, we found that H‐Ras, which is known to drive EMT , was markedly up‐regulated in Tn‐positive cells, whereas knockdown of H‐Ras suppressed Tn antigen induced activation of EMT . Furthermore, we confirmed that LS 174T cells (Tn‐positive) transfected with wild‐type Cosmc, thus expressing no Tn antigen, had down‐regulation of H‐Ras expression and subsequent inhibition of EMT process. In addition, analysis of 438 samples in TCGA cohort demonstrated that Cosmc expression was reversely correlated with H‐Ras, underscoring the significance of Tn antigen‐H‐Ras signalling in CRC patients. These data demonstrated that Cosmc deletion‐mediated Tn antigen exposure promotes CRC metastasis, which is possibly mediated by H‐Ras‐induced EMT activation.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Tn antigen promotes human colorectal cancer metastasis via H‐Ras mediated epithelial‐mesenchymal transition activation

Liu

Dong

et al. 2019

J Cellular Molecular Medi

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“…The quantity of treated sample however could greatly vary and range from 600 mg of intact protein (Hoja-Łukowicz et al, 2018) to 150 μg of tryptic digests (Zhou et al, 2017). Lectin dSPE followed by MS analysis has been applied for quantitative and/or qualitative studies of the glycoproteome, with an overall improvement in the number of the identified sites of glycosylation and of the monitoring of the abundance of those glycoproteins that can be used as biomarkers compared to the non-enriched proteome (Song et al, 2014(Song et al, , 2014Wang et al, 2014Wang et al, , 2016Bu et al, 2017;Hoja-Łukowicz et al, 2018;Patil et al, 2019). It's also worthwhile noting some protocols aiming to the further improvement of the coverage of glycosylation capturing.…”

Section: Dispersive Solid Phase Extraction With Commercial Lectin-based Sorbentsmentioning

confidence: 99%

Recent Advances in Lectin-Based Affinity Sorbents for Protein Glycosylation Studies

Goumenou

Delaunay

Pichon

2021

Front. Mol. Biosci.

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Glycosylation is one of the most significant post-translational modifications occurring to proteins, since it affects some of their basic properties, such as their half-life or biological activity. The developments in analytical methodologies has greatly contributed to a more comprehensive understanding of the quantitative and qualitative characteristics of the glycosylation state of proteins. Despite those advances, the difficulty of a full characterization of glycosylation still remains, mainly due to the complexity of the glycoprotein and/or glycopeptide mixture especially when they are present in complex biological samples. For this reason, various techniques that allow a prior selective enrichment of exclusively glycosylated proteins or glycopeptides have been developed in the past and are coupled either on- or off- line with separation and detection methods. One of the most commonly implemented enrichment methods includes the use of lectin proteins immobilized on various solid supports. Lectins are a group of different, naturally occurring proteins that share a common characteristic, which concerns their affinity for specific sugar moieties of glycoproteins. This review presents the different formats and conditions for the use of lectins in affinity chromatography and in solid phase extraction, including their use in dispersive mode, along with the recent progress made on either commercial or home-made lectin-based affinity sorbents, which can lead to a fast and automated glycosylation analysis.

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“…Glikoproteiny komórek nowotworowych charakteryzują się nadekspresją niekompletnych O-glikanów takich jak antygen T (rdzeń 1 O-glikanów: Galβ1-3GalNAc), antygen Tn (GalNAc) oraz ich sjalowanych form (odpowiednio sT oraz sTn) (Ryc. 2B) [1,30]. Antygen sTn określany jest jako antygen onkopłodowy -jego naturalnie wysoka ekspresja ograniczona jest do tkanek embrionalnych, ale również ulega on nadekspresji w komórkach zmienionych nowotworowo w porównaniu do ich zdrowych odpowiedników.…”

Section: Glikozylacja Białek W Komórkach Nowotworowychunclassified

Rola glikanów w rozwoju i progresji nowotworu. Zastosowania kliniczne

Grzesik¹,

Hoja-Łukowicz

2021

Postepy Biochem

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Charakterystyczną cechą złośliwienia komórek guza jest zmiana wzoru glikozylacji glikokaliksu powierzchni komórkowych, glikokoniugatów wydzielanych do płynów ustrojowych oraz glikokoniugatów macierzy zewnątrzkomórkowej. Zmiany glikozylacji są wynikiem mutacji genetycznych związanych z procesem nowotworzenia jak i zmian epigenetycznych zachodzących w środowisku guza, w tym zmian związanych z napływem składników odżywczych, ciśnieniem parcjalnym tlenu, ekspresją cytokin i stymulacją długotrwałych stanów zapalnych. Unikalny zestaw powierzchniowych antygenów cukrowych na komórkach nowotworowych rozpoznawany jest przez endogenne lektyny zlokalizowane w macierzy zewnątrzkomórkowej, śródbłonku naczyniowym, na leukocytach czy płytkach krwi i ma wpływ na zaburzanie podstawowych procesów związanych z zachowaniem się komórki, takich jak rozpoznawanie i adhezja międzykomórkowa czy oddziaływania komórka−składniki macierzy zewnątrzkomórkowej. Zmiany te mają krytyczny wpływ na potencjał migracyjny, inwazyjny i przerzutowy komórek nowotworowych oraz modulują odpowiedź immunologiczną. Ten unikalny wzór antygenów cukrowych powierzchni komórek nowotworowych odróżnia je od komórek prawidłowych i może być wykorzystany do ich identyfikacji, określenia zaawansowania choroby jak również stanowić cel terapii antynowotworowej.

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Proteomic analysis of Tn-bearing glycoproteins from different stages of melanoma cells reveals new biomarkers

Cited by 7 publications

References 90 publications

Tn antigen promotes human colorectal cancer metastasis via H‐Ras mediated epithelial‐mesenchymal transition activation

Tn antigen promotes human colorectal cancer metastasis via H‐Ras mediated epithelial‐mesenchymal transition activation

Recent Advances in Lectin-Based Affinity Sorbents for Protein Glycosylation Studies

Rola glikanów w rozwoju i progresji nowotworu. Zastosowania kliniczne

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