Proteomic and metabolomic profiling of urine uncovers immune responses in patients with COVID-19

Bi, Xiaojie; Liu, Wei; Ding, Xuan; Liang, Shuang; Zheng, Yufen; Zhu, Xiaoli; Quan, Sheng; Yi, Xiao; Xiang, Nan; Du, Juping; Lyu, Haiyan; Yu, Die; Zhang, Chao; Xu, Luang; Ge, Weigang; Zhan, Xin-Ke; He, Jiale; Xiong, Zi Jian; Zhang, Shun; Li, Yanchang; Xu, Ping; Zhu, Guangjun; Wang, Donglian; Zhu, Hongying; Chen, Shiyong; Li, Jun; Zhao, Haihong; Zhu, Yi; Liu, Huafen; Xu, Jiaqin; Shen, Bo; Guo, Tiannan

doi:10.1016/j.celrep.2021.110271

Cited by 98 publications

(86 citation statements)

References 76 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…This may be attributable to many factors, including the time point of testing and different proportion of severe cases in studied countries, and/or different scales of patient sampling. Furthermore, several publications reported the important role of urine tests in evaluating the severity, occurrence of AKI, and clinical course of COVID-19 patients, supporting the utility of urine as an informative biospecimen [29][30][31].…”

Section: Discussionmentioning

confidence: 92%

Urinalysis, but Not Blood Biochemistry, Detects the Early Renal Impairment in Patients with COVID-19

et al. 2022

View full text Add to dashboard Cite

Background: Coronavirus 2019 (COVID-19), caused by the severe acute respiratory syndrome coronavirus (SARS-CoV-2), has created a tremendous economic and medical burden. The prevalence and prognostic value of SARS-CoV-2-induced kidney impairment remain controversial. The current study aimed to provide additional evidence on the incidence of acute kidney injury (AKI) in COVID-19 patients and propose the use of urinalysis as a tool for screening kidney impairment. Methods: 178 patients with confirmed COVID-19 were enrolled in this retrospective cohort study. The laboratory examinations included routine blood tests, blood biochemical analyses (liver function, renal function, lipids, and glucose), blood coagulation index, lymphocyte subset and cytokine analysis, urine routine test, C-reactive protein, erythrocyte sedimentation, and serum ferritin. Results: No patient exhibited a rise in serum creatinine or Cystatin C and occurrence of AKI, and only 2.8% of patients were recorded with an elevated level of blood urea nitrogen among all cases. On the contrary, 54.2% of patients who underwent routine urine testing presented with an abnormal urinalysis as featured by proteinuria, hematuria, and leucocyturia. Conclusions: Kidney impairment is prevalent among COVID-19 patients, with an abnormal urinalysis as a clinical manifestation, implying that a routine urine test is a stronger indication of prospective kidney complication than a blood biochemistry test.

show abstract

Section: Discussionmentioning

confidence: 92%

Urinalysis, but Not Blood Biochemistry, Detects the Early Renal Impairment in Patients with COVID-19

et al. 2022

View full text Add to dashboard Cite

show abstract

“…Notably, 80% of the thus measured serum proteins were detectable in urine, while only 31% of urinary proteins were detected in serum. 5 …”

Section: Main Textmentioning

confidence: 99%

“… 4 Urinary protein-derived models performed as well as those from sera and could be used to monitor COVID-19 disease progression. 5 …”

Section: Main Textmentioning

confidence: 99%

Multi-omics in COVID-19: Seeing the unseen but overlooked in the clinic

Tian

Wang

Guo

2022

Cell Reports Medicine

Self Cite

View full text Add to dashboard Cite

“…Nowadays, urine is being widely studied in clinical omics as it can be obtained noninvasively in large quantities and is stable in vitro. Urinary metabolomics has been studied in many infectious diseases, such as COVID-19 infection, Lyme disease and Schistosoma infection [15][16][17]. However, up to now, no such study has ever been performed on toxoplasmosis.…”

Section: Introductionmentioning

confidence: 99%

Investigation of urine metabolome of BALB/c mouse infected with an avirulent strain of Toxoplasma gondii

Zhou

Huang

et al. 2022

Parasites Vectors

View full text Add to dashboard Cite

Background The protozoan parasite Toxoplasma gondii is a major concern for human and animal health. Although the metabolic understanding of toxoplasmosis has increased in recent years, the analysis of metabolic alterations through noninvasive methodologies in biofluids remains limited. Methods Here, we applied liquid chromatography-tandem mass spectrometry (LC-MS/MS)-based metabolomics and multivariate statistical analysis to analyze BALB/c mouse urine collected from acutely infected, chronically infected and control subjects. Results In total, we identified 2065 and 1409 metabolites in the positive electrospray ionization (ESI +) mode and ESI − mode, respectively. Metabolomic patterns generated from principal component analysis (PCA) and partial least squares discriminant analysis (PLS-DA) score plots clearly separated T. gondii-infected from uninfected urine samples. Metabolites with altered levels in urine from T. gondii-infected mice revealed changes in pathways related to amino acid metabolism, fatty acid metabolism, and nicotinate and nicotinamide metabolism. Conclusions This is the first study to our knowledge on urine metabolic profiling of BALB/c mouse with T. gondii infection. The urine metabolome of infected mouse is distinctive and has value in the understanding of Toxoplasmosis pathogenesis and improvement of treatment. Graphical Abstract

show abstract

Proteomic and metabolomic profiling of urine uncovers immune responses in patients with COVID-19

Cited by 98 publications

References 76 publications

Urinalysis, but Not Blood Biochemistry, Detects the Early Renal Impairment in Patients with COVID-19

Urinalysis, but Not Blood Biochemistry, Detects the Early Renal Impairment in Patients with COVID-19

Multi-omics in COVID-19: Seeing the unseen but overlooked in the clinic

Investigation of urine metabolome of BALB/c mouse infected with an avirulent strain of Toxoplasma gondii

Contact Info

Product

Resources

About