Proteomic and Properties Analysis of Botanical Insecticide Rhodojaponin III-Induced Response of the Diamondback Moth, Plutella xyllostella (L.)

Abstract

BackgroundRhodojaponin III, as a botanical insecticide, affects a wide variety of biological processes in insects, including reduction of feeding, suspension of development, and oviposition deterring of adults in a dose-dependent manner. However, the mode of these actions remains obscure.Principal FindingsIn this study, a comparative proteomic approach was adopted to examine the effect of rhodojaponin III on the Plutella xyllostella (L.). Following treating 48 hours, newly emergence moths were collected and protein samples were prepared. The proteins were separated by 2-DE, and total 31 proteins were significantly affected by rhodojaponin III compared to the control identified by MALDI-TOF/TOF-MS/MS. These differentially expressed proteins act in the nervous transduction, odorant degradation and metabolic change pathways. Further, gene expression patterns in treated and untreated moths were confirmed by qRT-PCR and western blot analysis. RNAi of the chemosensory protein (PxCSP) gene resulted in oviposition significantly increased on cabbage plants treated with rhodojaponin III.ConclusionsThese rhodojaponin III-induced proteins and gene properties analysis would be essential for a better understanding of the potential molecular mechanism of the response to rhodojaponin III from moths of P. xylostella.

Proteomics as a tool for tapping potential of entomopathogens as microbial insecticides

“…Both proteomic approaches have been increasingly adopted for insect pest management research (Table ). The examples of applications include insect–plant host interactions (Dastranj, Gharechahi, & Salekdeh, ), characterizing mechanism of action of synthetic and bio‐based insecticides (Dong et al, ; Erban, Harant, Chalupnikova, Kocourek, & Stara, ; J. M. Park et al, ; Roat et al, ), assessing impact of insecticides of nontarget organisms (Vioque‐Fernández, De Almeida, & López‐Barea, ), and characterizing EPM in managing insect pests. Contemporary proteomics research has the options of adopting gel‐based or MS‐based, labeled or nonlabelled approach to answer biological questions (Figure ).…”

Optimization Method for Proteomic Analysis of the Larva and Adult Tissues of Plutella xylostella (L.) (Lepidoptera: Plutellidae)

“…Recent studies on insect proteomics have given insight into the molecular mechanisms of larva development, diapauses and resistance mechanisms (Colinet et al 2012;Konus et al 2013;Li et al 2014a;Yin et al 2017;Zhang et al 2013). Differential proteomics which aimed to understand protein regulation during normal and treatment status were applied in several insecticides resistance and susceptibility response studies of P. xylostella (Dong et al 2013;Xie et al 2011;Yin et al 2017;Zhang et al 2013). These results suggested that the insecticides treatment have induced alteration in protein, which are participated in the nervous transduction and multiple metabolic pathways.…”

“…xylostella (Xie et al 2011) while tris lysis buffer methods were applied to extract the protein from P. xylostella adult tissues (Dong et al 2013). In addition, TCA-acetone with dithiothreitol (DTT) was used to extract the protein from P. xylostella adult (Zhang et al 2013) and larva (Yin et al 2017) tissues.…”

“…Compared to the optimal methods for the extraction of plant tissues, only a few studies in the literature that compare different extraction methods for insect tissues (Cilia et al 2009;Collet & Baguñà 1993;Qin et al 2013;Wang et al 2010;Yiou et al 2013), particularly for larva and adult tissues of P. xylostella. The major drawback in proteomics research of P. xylostella is the lack of reliable protein extraction methods (Dong et al 2013;Li et al 2014a;Xie et al 2011;Yin et al 2017;Zhang et al 2013). Thus, the development of efficient protein extraction methods for larva and adult tissues of P. xylostella will expedite proteomic studies of this Lepidoptera pest.…”

“…The aim of the present study was to find an optimal method for extracting the total protein of P. xylostella for subsequent one-dimensional electrophoresis (1-DE) and 2-DE analyses. We employed five different extraction methods (Cilia et al 2009;Dong et al 2013;Li et al 2014b;Xie et al 2011;Zhang et al 2013) on both larva and adults of P. xylostella. These methods were chosen based on their successful used previously for extracting proteins from various insect samples including P. xylostella, albeit from different types of tissues or stages.…”