Proteomic and structural characterization of self-assembled vesicles from excretion/secretion products of Toxoplasma gondii

Ramírez-Flores, Carlos J; Cruz-Mirón, Rosalba; Mondragón-Castelán, Mónica; González-Pozos, Sirenia; Ríos-Castro, Emmanuel; Mondragón-Flores, Ricardo

doi:10.1016/j.jprot.2019.103490

Cited by 25 publications

(24 citation statements)

References 54 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Sodium compound at different concentrations and pH values have been shown to induce plasma membrane vesiculation in T. cruzi epimastigote (Da Silveira et al, 1979 ). Remarkably, purified tachyzoites of Toxoplasma gondii maintained in PBS at 37°C were able to secrete vesicles (Ramírez-Flores et al, 2019 ), but from the report of Barbosa et al ( 2018 ), a temperature of 37°C impaired the secretion, biological effects, and cargo content of EVs released by L. amazonensis promastigote in culture.…”

Section: Stressors For Ppev Secretionmentioning

confidence: 99%

“…Relatively, centrifugation steps can disorganize tubules into vesicles similar to ectosomes and exosomes (Ramírez-Flores et al, 2019 ), indicating that laboratory treatment may influence extant populations of EVs, but whether tubule disorganization into the vesicle by gravitational shearing represents EV activation through cell–cell abrasions or cell–parasite contacts requires further clarification. Nonetheless, other stressors that can initiate the secretion of PPEVs include cell topography, apoptosis or autophagy (Yanez-Mo' et al, 2015 ), hypoxia, and irradiation (Torró et al, 2018 ).…”

Section: Stressors For Ppev Secretionmentioning

confidence: 99%

“…The sequence of PPEV internalization might involve binding to host cells through receptor–ligand interactions, and in the process become putatively attached to the target-cell membranes before endocytosis (Valadi et al, 2007 ). Ramírez-Flores et al ( 2019 ) reported that endosome-associated Rab proteins played some roles in the fusion of L. infantum vesicles to host cells and the formation of tubules even though electroporation of myelogenous leukemia cells with T. cruzi epimastigote EVs showed clear incorporation of labeled EVs following a series of endocytosis and exocytosis (Garcia-Silva et al, 2014 ) ( Figure 2 ).…”

Section: Mechanisms Of Ppevs Internalizationmentioning

confidence: 99%

“…The proteomic analysis of T. brucei -derived EVs speculated that the flagellum might play a considerable role in the sorting and delivery of its biologically active molecules to neighboring cells (Szempruch et al, 2016b ). T. gondii tachyzoite vesicles contained dense granular protein with an indication that dense granules and self-assembled vesicle-tubular structures are a potential source of proteins in the vesicle (Ramírez-Flores et al, 2019 ). Essentially, properties of L. donovani wild-type and mutant strain vesicles were influenced by the specificities of cargo packaging regulated by HSP100 (Silverman et al, 2010b ).…”

Section: Ppevs Bioactive Molecules: Exports and Functionsmentioning

confidence: 99%

“…Essentially, properties of L. donovani wild-type and mutant strain vesicles were influenced by the specificities of cargo packaging regulated by HSP100 (Silverman et al, 2010b ). Additionally, the putative pantothenate protein (PAT) and HSP100 of P. berghei secretory vesicles were necessary for the expulsion of vesicular content into the parasitophorous vacuole (Kehrer et al, 2016 ), just as extracellular T. gondii tachyzoites constitutively secreted soluble components of the vesicles within the parasitophorous vacuole (Ramírez-Flores et al, 2019 ). Meanwhile, a subtle contrast has been found in T. cruzi where the predominant EV proteins were likely anchored on the parasite surface via GPI lipid or inserted into the EV membrane past a conserved C-terminal region (Bautista-lópez et al, 2017 ).…”

Section: Ppevs Bioactive Molecules: Exports and Functionsmentioning

confidence: 99%

See 4 more Smart Citations

Perils and Promises of Pathogenic Protozoan Extracellular Vesicles

Olajide

Cai

2020

Front. Cell. Infect. Microbiol.

View full text Add to dashboard Cite

Extracellular vesicles (EVs) are membranous structures formed during biological processes in living organisms. For protozoan parasites, secretion of EVs can occur directly from the parasite organellar compartments and through parasite-infected or antigen-stimulated host cells in response to in vitro and in vivo physiological stressors. These secreted EVs characteristically reflect the biochemical features of their parasitic origin and activating stimuli. Here, we review the species-specific morphology and integrity of parasitic protozoan EVs in concurrence with the origin, functions, and internalization process by recipient cells. The activating stimuli for the secretion of EVs in pathogenic protozoa are discoursed alongside their biomolecules and specific immune cell responses to protozoan parasite-derived EVs. We also present some insights on the intricate functions of EVs in the context of protozoan parasitism.

show abstract

Section: Stressors For Ppev Secretionmentioning

confidence: 99%

Section: Stressors For Ppev Secretionmentioning

confidence: 99%

Section: Mechanisms Of Ppevs Internalizationmentioning

confidence: 99%

Section: Ppevs Bioactive Molecules: Exports and Functionsmentioning

confidence: 99%

Section: Ppevs Bioactive Molecules: Exports and Functionsmentioning

confidence: 99%

See 3 more Smart Citations

Perils and Promises of Pathogenic Protozoan Extracellular Vesicles

Olajide

Cai

2020

Front. Cell. Infect. Microbiol.

View full text Add to dashboard Cite

show abstract

Unraveling the relevance of the polyadenylation factor EhCFIm25 in Entamoeba histolytica through proteomic analysis

et al. 2021

View full text Add to dashboard Cite

We recently reported that silencing of the polyadenylation factor EhCFIm25 in Entamoeba histolytica, the protozoan which causes human amoebiasis, affects trophozoite proliferation, death, and virulence, suggesting that EhCFIm25 may have potential as a new biochemical target. Here, we performed a shotgun proteomic analysis to identify modulated proteins that could explain this phenotype. Data are available via ProteomeXchange with identifier PXD027784. Our results revealed changes in the abundance of 75 proteins. Interestingly, STRING analysis, functional GO-term annotations, KEGG analyses and literature review showed that modulated proteins are mainly related to glycolysis and carbon metabolism, cytoskeleton dynamics and parasite virulence, as well as gene expression and protein modifications. Further studies are needed to confirm the hypotheses emerging from this proteomic analysis, to thereby acquire a comprehensive view of the molecular mechanisms involved.

show abstract

Guidelines for the purification and characterization of extracellular vesicles of parasites

Fernandez‐Becerra,

Xander,

Alfandari

et al. 2023

J of Extracellular Bio

View full text Add to dashboard Cite

Parasites are responsible for the most neglected tropical diseases, affecting over a billion people worldwide (WHO, 2015) and accounting for billions of cases a year and responsible for several millions of deaths. Research on extracellular vesicles (EVs) has increased in recent years and demonstrated that EVs shed by pathogenic parasites interact with host cells playing an important role in the parasite's survival, such as facilitation of infection, immunomodulation, parasite adaptation to the host environment and the transfer of drug resistance factors. Thus, EVs released by parasites mediate parasite‐parasite and parasite‐host intercellular communication. In addition, they are being explored as biomarkers of asymptomatic infections and disease prognosis after drug treatment. However, most current protocols used for the isolation, size determination, quantification and characterization of molecular cargo of EVs lack greater rigor, standardization, and adequate quality controls to certify the enrichment or purity of the ensuing bioproducts. We are now initiating major guidelines based on the evolution of collective knowledge in recent years. The main points covered in this position paper are methods for the isolation and molecular characterization of EVs obtained from parasite‐infected cell cultures, experimental animals, and patients. The guideline also includes a discussion of suggested protocols and functional assays in host cells

show abstract

Proteomic and structural characterization of self-assembled vesicles from excretion/secretion products of Toxoplasma gondii

Cited by 25 publications

References 54 publications

Perils and Promises of Pathogenic Protozoan Extracellular Vesicles

Perils and Promises of Pathogenic Protozoan Extracellular Vesicles

Unraveling the relevance of the polyadenylation factor EhCFIm25 in Entamoeba histolytica through proteomic analysis

Guidelines for the purification and characterization of extracellular vesicles of parasites

Contact Info

Product

Resources

About