Proteomic and Transcriptomic Analyses to Decipher the Chitinolytic Response of Jeongeupia spp.

The conventional methods used to produce N-acetyl-β-D-glucosamine (GlcNAc) from seafood waste require pretreatment steps that use acids or bases to achieve the extraction and decrystallization of chitin prior to enzymatic conversion. The development of an enzymatic conversion method that does not require the pretreatment of seafood waste is essential for the efficient and clean production of GlcNAc. In this study, the annotated metagenomic assembly data of domesticated microbiota (XHQ10) were analyzed to identify carbohydrate-active enzymes (CAZymes), and an in-depth analysis of the high-quality genome FS13.1, which was obtained from metagenomic binning, was performed; this enabled us to elucidate the catabolic mechanism of XHQ10 by using shrimp shell chitin as a carbon and nitrogen source. The only β-N-acetylglucosaminidase (named XmGlcNAcase) was cloned from FS13.1 and biochemically characterized. The direct production of GlcNAc from shrimp shell powder (SSP) via the use of a chitin enzyme cocktail was evaluated. Under the action of a chitin enzyme cocktail containing 5% recombinant XmGlcNAcase and a crude XHQ10 enzyme solution, the yield and purity of the final conversion of SSP to GlcNAc were 2.57 g/L and 82%, respectively. This is the first time that metagene-derived GlcNAcase has been utilized to achieve the enzymatic conversion of untreated seafood waste, laying the foundation for the low-cost and sustainable production of GlcNAc.

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High Degree of Polymerization of Chitin Oligosaccharides Produced from Shrimp Shell Waste by Enrichment Microbiota Using Two-Stage Temperature-Controlled Technique of Inducing Enzyme Production and Metagenomic Analysis of Microbiota Succession

Pan,

Xiao,

et al. 2024

Marine Drugs

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The direct enzymatic conversion of untreated waste shrimp and crab shells has been a key problem that plagues the large-scale utilization of chitin biological resources. The microorganisms in soil samples were enriched in two stages with powdered chitin (CP) and shrimp shell powder (SSP) as substrates. The enrichment microbiota XHQ10 with SSP degradation ability was obtained. The activities of chitinase and lytic polysaccharide monooxygenase of XHQ10 were 1.46 and 54.62 U/mL. Metagenomic analysis showed that Chitinolyticbacter meiyuanensis, Chitiniphilus shinanonensis, and Chitinimonas koreensis, with excellent chitin degradation performance, were highly enriched in XHQ10. Chitin oligosaccharides (CHOSs) are produced by XHQ10 through enzyme induction and two-stage temperature control technology, which contains CHOSs with a degree of polymerization (DP) more significant than ten and has excellent antioxidant activity. This work is the first study on the direct enzymatic preparation of CHOSs from SSP using enrichment microbiota, which provides a new path for the large-scale utilization of chitin bioresources.

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Proteomic and Transcriptomic Analyses to Decipher the Chitinolytic Response of Jeongeupia spp.

Cited by 3 publications

References 104 publications

Nanochitosan-Based Fish Disease Prevention and Control

Nanochitosan-Based Fish Disease Prevention and Control

Efficient Production of N-Acetyl-β-D-Glucosamine from Shrimp Shell Powder Using Chitinolytic Enzyme Cocktail with β-N-Acetylglucosaminidase from Domesticated Microbiome Metagenomes

High Degree of Polymerization of Chitin Oligosaccharides Produced from Shrimp Shell Waste by Enrichment Microbiota Using Two-Stage Temperature-Controlled Technique of Inducing Enzyme Production and Metagenomic Analysis of Microbiota Succession

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