Proteomic approach for investigation of cytoplasmic male sterility (CMS) in Brassica

Mihr, Christina; Baumgärtner, Maja; Dieterich, J.-H.; Schmitz, Udo K.; Braun, Hans‐Peter

doi:10.1078/0176-1617-00292

Cited by 33 publications

(16 citation statements)

References 21 publications

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“…The proteins up-regulated in the WT include a number of proteases, leucine-rich proteins, FtsZ protein, and translational and transcriptional factors, whereas in the mutant proteins involved in lipid and nucleotide metabolism, and protease inhibitors, in particular cystatin, are highly expressed and this correlates with abnormalities in tapetum and pollen development (Sheoran et al 2009a). In a study of the Tournefortii CMS system of B. napus, the protein profiles of anthers and seedlings on 2-D gels are presented, but the differential protein spots are not identified (Mihr et al 2001).…”

Section: Introductionmentioning

confidence: 97%

Proteome analysis of the normal and Ogura (ogu) CMS anthers of Brassica napus to identify proteins associated with male sterility

Sheoran

Sawhney

2010

Botany

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In the Ogura (ogu) cytoplasmic male sterility (CMS) system in Brassica napus L., male sterility is caused by a defect in the mitochondrial gene and, under our growth conditions, stamens produced are carpelloid. We analyzed the proteome of ogu and normal anthers with the objective of identifying differentially expressed proteins and their potential roles in pollen development and male sterility. By using differential in-gel electrophoresis, we detected over 2300 spots on 2-D gels of normal and ogu anthers and of the 167 spots with 2-fold or higher expression, 120 were analyzed by MALDI-TOF/TOF mass spectrometry and 94 proteins were identified searching against NCBI and Brassica EST databases. Proteins up-regulated in normal anthers included those associated with carbohydrate and energy metabolism (e.g., fructose bisphosphatase, ATP synthase, malate dehydrogenase, and cytochrome c oxidase), cell wall remodeling (e.g., b-1,3-glucanase and pectinesterase), aldehyde dehydrogenase (ALDH), photosynthesis, and flavonoid synthesis. Proteases were identified in both the normal and ogu anthers, but a number of protease inhibitors were up-regulated in ogu anthers. The activities of ALDH and b-1,3-glucanase were also much higher in normal anthers by comparison with ogu anthers. This study documents the proteomic basis of mitochondrial-nuclear interaction in the control of ogu CMS in B. napus.Résumé : Dans le système Ogura (ogu) de stérilité mâle (CMS) chez le Brassica napus L., la stérilité mâle est causée par un défaut du gène mitochondrial, et sous les conditions de croissance utilisées, les étamines sont carpelloïdes. Les auteurs ont analysé le protéome des anthères ogu et normales afin d'identifier des protéines différentiellement exprimées, ainsi que leurs rôles potentiels dans le développement du pollen et de la stérilité mâle. En utilisant l'électrophorèse différentielle sur gel, ils ont détecté plus de 2300 taches sur gels 2-D pour les anthères ogu et normales, et sur les 167 taches avec une expression double ou plus, ils en ont analysé 120 par spectrométrie de masse MALDI-TOF/TOF pour finalement identifier 94 protéines en utilisant les bases de données NCBI et Brassica EST. Les protéines positivement régulées dans les anthè-res normales, incluent celles associées avec le métabolisme des glucides et de l'énergie (e.g., fructose diphosphate, ATP synthase, malate déshydrogénase, et cytochrome oxydase), remodelage des parois cellulaires (e.g. b-1,3 glucanase et pectinestérase), aldéhyde déshydrogénase (ALDH), photosynthèse, et synthèse des flavonoïdes. Ils ont identifié les protéases chez les anthères normales aussi bien que de type ogu, mais le nombre d'inhibiteurs des protéases est régulé positivement chez les anthères ogu. Les activités des ALDH et de la b-1,3 glucanase sont également plus importantes chez les anthères normales comparativement aux anthères ogu. Cette étude présente la base protéomique de l'interaction noyau-mitochondries dans le contrôle du CMS ogu chez le B. napus.Mots-clés : Brassica napus, stérilit...

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Section: Introductionmentioning

confidence: 97%

Proteome analysis of the normal and Ogura (ogu) CMS anthers of Brassica napus to identify proteins associated with male sterility

Sheoran

Sawhney

2010

Botany

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“…As a powerful tool that can globally detect genes related to CMS, proteomics have been applied in several plants, including Arabidopsis (Lutziger and Oliver, 2001;Fujiki et al, 2002;Noir et al, 2005;Grobei et al, 2009;Cui et al, 2011), tomato (Sheoran et al, 2007;Sheoran et al, 2009b), rice (Imin et al, 2001;Kerim et al, 2003;Sun et al, 2009), Brassica napus (Mihr et al, 2001;Sheoran et al, 2009a;Sheoran and Sawhney, 2010), and wolfberry (Zheng et al, 2012). In addition, many important proteins related to CMS have been identified and found to be involved in complex pathways.…”

Section: Introductionmentioning

confidence: 98%

Identification of proteins associated with cytoplasmic male sterility in pepper ( Capsicum annuum L.)

Zhang

Chen

Zhang

et al. 2015

South African Journal of Botany

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a b s t r a c t Edited by E BalazsKeywords: Pepper Cytoplasmic male sterility Protein Identification Two dimension difference gel electrophoresis Pepper (Capsicum annuum L.) is a globally important and often cross-pollinated vegetable that exhibits significant heterosis. Though cytoplasmic male sterility (CMS) has an important value for pepper hybrid production, its molecular mechanism remains unclear. To explore the molecular mechanism of CMS in pepper at the proteome level, we examined the anther proteomes of a cytoplasmic male sterile line FS1030A and its maintainer line FS1030B by transmission electronic microscopy (TEM) during abortion at the microspore mother cell stage. Two dimension difference gel electrophoresis (2D-DIGE) and liquid chromatography-tandem mass spectrometry (LC-MS/MS) analyses were used to determine differentially expressed proteins. About 1070 anther protein spots were detected on each gel, and 13 protein spots were expressed differently between FS1030A and FS1030B (p b 0.05). Twelve of the thirteen protein spots (92.3%) were successfully identified by NCBInr as nine proteins, namely down-regulated triosephosphate isomerase, actin, glutathione S-transferase (GST), Cu/Zn superoxide dismutase (Cu/Zn-SOD), and up-regulated branched-chain α-keto acid dehydrogenase E3 subunit, ketol-acid reductoisomerase (KARI), aspartic protease, and nascent polypeptides. The low-expression of triosephosphate isomerase involved in glycolysis impacts oxidative phosphorylation significantly and ultimately results in a reduction in ATP production, and over-expression of aspartic protease blocking normal programmed cell death (PCD) played important roles in the occurrence of CMS-FS1030A. This is the first study on pepper CMS using a proteomic approach. These findings provide a good basis for further studies of CMS in pepper.

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“…However, these studies focused on the proteome at whole cell level. It has been shown that the mitochondrial protein represents only a minor part of total proteins [16,24]. Therefore a proteomic approach using total protein for the analysis of PCD has severe limitations due to large quantitative differences between abundant cellular proteins and rare mitochondrial polypeptides.…”

Section: Introductionmentioning

confidence: 99%

Mitochondrial proteome during salt stress-induced programmed cell death in rice

Chen

Wang

et al. 2009

Plant Physiology and Biochemistry

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Proteomic approach for investigation of cytoplasmic male sterility (CMS) in Brassica

Cited by 33 publications

References 21 publications

Proteome analysis of the normal and Ogura (ogu) CMS anthers of Brassica napus to identify proteins associated with male sterility

Proteome analysis of the normal and Ogura (ogu) CMS anthers of Brassica napus to identify proteins associated with male sterility

Identification of proteins associated with cytoplasmic male sterility in pepper ( Capsicum annuum L.)

Mitochondrial proteome during salt stress-induced programmed cell death in rice

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