Proteomic Approach to Reveal the Regulatory Function of Aconitase AcnA in Oxidative Stress Response in the Antibiotic Producer Streptomyces viridochromogenes Tü494

Michta, Ewelina; Ding, Wei; Zhu, Shaochun; Blin, Kai; Ruan, Hongqiang; Wang, Rui; Wohlleben, Wolfgang; Mast, Yvonne

doi:10.1371/journal.pone.0087905

Cited by 16 publications

(16 citation statements)

References 75 publications

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“…Further experimentation is necessary to determine the effect of AcnB on these transcripts. Since aconitase has been shown to posttranscriptionally regulate a variety of genes in response to oxidative stress (6,8), we were interested in determining if aconitase regulates proteins directly involved in the oxidative stress response in H. pylori. By exposing cells to 12% O 2 , we hoped to reveal some novel targets for aconitase-mediated modulation.…”

Section: Discussionmentioning

confidence: 99%

“…Known IRE and IRE-like patterns, including CAGUG, CAGCG, GAGAG, GGGAG, CAGGG, and CUGUG (6,24), were used as input sequences for the Pattern Locator program (25). The program was set to search the H. pylori 26695_uid57787 DNA sequence within the intergenic regions of the plus (default) and minus DNA strands.…”

Section: Methodsmentioning

confidence: 99%

“…The regulatory role of aconitase has been studied in several different bacteria, and these bacterial aconitases have been shown to bind not only to the eukaryotic consensus IRE but also to several IRE-like sequences (4)(5)(6). In Escherichia coli, AcnA and AcnB have been shown to bind to and subsequently regulate the expression of the acnA and acnB transcripts, respectively (7).…”

mentioning

confidence: 99%

“…Aconitase in Bacillus subtilis (CitB) binds to the gerE transcript, a gene involved in sporulation (4). Streptomyces viridochromogenes Tu494 AcnA binds to the recombinase A transcript (recA) and to ftsZ, a cell division gene involved in sporulation, and several additional targets for AcnA were identified in S. viridochromogenes Tu494 when cells were exposed to oxidative stress (6). Additionally, Acn of Mycobac-terium tuberculosis binds to the thioredoxin (trx) transcript and to ideR, an iron-dependent regulator (10).…”

mentioning

confidence: 99%

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Aconitase Functions as a Pleiotropic Posttranscriptional Regulator in Helicobacter pylori

2015

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Posttranscriptional regulation in bacteria has increasingly become recognized as playing a major role in response to environmental stimuli. Aconitase is a bifunctional protein that not only acts enzymatically but also can be a posttranscriptional regulator. To investigate protein expression regulated by Helicobacter pylori AcnB in response to oxidative stress, a global proteomics study was conducted wherein the ⌬acnB strain was compared to the parent strain when both strains were O 2 stressed. Many proteins, including some involved in urease activity, in combating oxidative stress, and in motility, were expressed at a significantly lower level in the ⌬acnB strain. A bioinformatics prediction tool was used to identify putative targets for aconitase-mediated regulation, and electrophoretic mobility shift assays demonstrated that apo-AcnB is able to bind to RNA transcripts of hpn (encoding a nickel-sequestering protein), ahpC (encoding alkyl hydroperoxide reductase), and flgR (encoding flagellum response regulator). Compared to the wild type (WT), the ⌬acnB strain had decreased activities of the nickel-containing enzymes urease and hydrogenase, and this could be correlated with lower total nickel levels within ⌬acnB cells. Binding of apo-AcnB to the hpn 5= untranslated region (UTR) may inhibit the expression of Hpn. In agreement with the finding that AcnB regulates the expression of antioxidant proteins such as AhpC, ⌬acnB cells displayed oxidative-stress-sensitive phenotypes. The ⌬acnB strain has a lesser motility ability than the WT strain, which can likely be explained by the functions of AcnB on the FlgRS-RpoN-FlgE regulatory cascade. Collectively, our results suggest a global role for aconitase as a posttranscriptional regulator in this gastric pathogen. IMPORTANCEBacterial survival depends on the ability of the cell to sense and respond to a variety of environmental changes. For Helicobacter pylori, responding to environmental stimuli within the gastric niche is essential for persistence and host colonization. However, there is much to be learned about the regulatory mechanisms that H. pylori employs to orchestrate its response to different stimuli. In this study, we explore the role of aconitase, a bifunctional protein that has been found to act as a posttranscriptional regulator in several other bacteria. Our results shed light on the magnitude of aconitase-mediated regulation in H. pylori, and we propose that aconitase acts as a global regulator of key genes involved in virulence.

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Section: Discussionmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

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confidence: 99%

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confidence: 99%

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Aconitase Functions as a Pleiotropic Posttranscriptional Regulator in Helicobacter pylori

2015

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“…This plant process can be subverted by enzymatic machinery related to antioxidant activity; e.g., catalase and superoxide dismutase by S. coelicolor, and aconitase by S. viridochromogenes strain Tü494. This process plays an important role in alleviating the cytotoxic effects induced by reactive oxygen species and in allowing successful plant tissue colonization [97][98][99].…”

Section: Ecology Of Streptomyces-plant Host Interactionmentioning

confidence: 99%

Plant growth promotion by streptomycetes: ecophysiology, mechanisms and applications

Sousa

Olivares

2016

Chem. Biol. Technol. Agric.

128

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The genus Streptomyces comprises filamentous Gram-positive bacteria that are widely recognized for their ability to produce bioactive compounds such as antimicrobial, antiparasitic and immune-suppressing compounds via secondary metabolism. These bioactive compounds represent a third of all commercially available antibiotics. Streptomycetes have been found in beneficial associations with plants where they have improved plant growth and protected against pests, which have attracted the attention of researchers worldwide. This review focuses on the potential of streptomycetes as plant growth-promoting bacteria (PGPS) and considers features related to secondary metabolic pathways, interactions with host plants and recent advances in elucidating plant growth-promoting mechanisms. Such advances in basic knowledge have increased the prospects for streptomycetes to be used as bioinoculants for sustainable agriculture.

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Proteomic insights into metabolic adaptation to deletion of metE in Saccharopolyspora spinosa

Yang

et al. 2015

Appl Microbiol Biotechnol

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Saccharopolyspora spinosa can produce spinosad as a major secondary metabolite, which is an environmentally friendly agent for insect control. Cobalamin-independent methionine synthase (MetE) is an important enzyme in methionine biosynthesis, and this enzyme is probably closely related to spinosad production. In this study, its corresponding gene metE was inactivated, which resulted in a rapid growth and glucose utilisation rate and almost loss of spinosad production. A label-free quantitative proteomics-based approach was employed to obtain insights into the mechanism by which the metabolic network adapts to the absence of MetE. A total of 1440 proteins were detected from wild-type and ΔmetE mutant strains at three time points: stationary phase of ΔmetE mutant strain (S1ΔmetE , 67 h), first stationary phase of wild-type strain (S1WT, 67 h) and second stationary phase of wild-type strain (S2WT, 100 h). Protein expression patterns were determined using an exponentially modified protein abundance index (emPAI) and analysed by comparing S1ΔmetE /S1WT and S1ΔmetE /S2WT. Results showed that differentially expressed enzymes were mainly involved in primary metabolism and genetic information processing. This study demonstrated that the role of MetE is not restricted to methionine biosynthesis but rather is involved in global metabolic regulation in S. spinosa.

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Proteomic Approach to Reveal the Regulatory Function of Aconitase AcnA in Oxidative Stress Response in the Antibiotic Producer Streptomyces viridochromogenes Tü494

Cited by 16 publications

References 75 publications

Aconitase Functions as a Pleiotropic Posttranscriptional Regulator in Helicobacter pylori

Aconitase Functions as a Pleiotropic Posttranscriptional Regulator in Helicobacter pylori

Plant growth promotion by streptomycetes: ecophysiology, mechanisms and applications

Proteomic insights into metabolic adaptation to deletion of metE in Saccharopolyspora spinosa

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