Proteomic‐based Techniques for the Characterization of Food Allergens

Picariello, Gianluca; Mamone, Gianfranco; Addeo, Francesco; Nitride, Chiara; Ferranti, Pasquale

doi:10.1002/9781118537282.ch3

Cited by 10 publications

(10 citation statements)

References 115 publications

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“…The linear ones are strictly related to the primary structure of the protein, are thermostable and can be recognized by antibodies even after the reduction process. The conformational ones are dependent on the secondary and tertiary protein structure, are thermolabile and their binding with antibodies is not possible after protein reduction . Proteomics can contribute in two ways to determine food allergens; a gel‐based approach or a gel‐free approach.…”

Section: Food Safetymentioning

confidence: 99%

Proteomics in food: Quality, safety, microbes, and allergens

et al. 2016

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Food safety and quality and their associated risks pose a major concern worldwide regarding not only the relative economical losses but also the potential danger to consumer's health. Customer's confidence in the integrity of the food supply could be hampered by inappropriate food safety measures. A lack of measures and reliable assays to evaluate and maintain a good control of food characteristics may affect the food industry economy and shatter consumer confidence. It is imperative to create and to establish fast and reliable analytical methods that allow a good and rapid analysis of food products during the whole food chain. Proteomics can represent a powerful tool to address this issue, due to its proven excellent quantitative and qualitative drawbacks in protein analysis. This review illustrates the applications of proteomics in the past few years in food science focusing on food of animal origin with some brief hints on other types. Aim of this review is to highlight the importance of this science as a valuable tool to assess food quality and safety. Emphasis is also posed in food processing, allergies, and possible contaminants like bacteria, fungi, and other pathogens.

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Section: Food Safetymentioning

confidence: 99%

Proteomics in food: Quality, safety, microbes, and allergens

et al. 2016

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“…The early successes of metabo[. ]omics as a top‐down approach in systems medicine and its very high promises for personalized healthcare and molecular epidemiology helped spread untargeted profiling to other research areas such as plant breeding, environmental impact, food and fraud detection, among others. Altogether, untargeted profiling accounts for a large part of complex mixtures studies.…”

Section: ‘Complex Mixtures’ By Nmrmentioning

confidence: 99%

“…Despite the statistical nature of this approach – a large number of samples is analyzed – the original raw data are still necessary. This becomes apparent when considering the complex sequence of transformations and filters applied to spectra prior to statistical analysis: Zero‐filling + apodization + Fourier transform + phase‐correction + baseline correction + alignment + normalization + centering + scaling, where alignment can be further expanded into complex operations that are well described in a recent review . In its simplest form: slight alignment can be achieved by lowering the spectral resolution dividing the spectra into bins (binning).…”

Section: ‘Complex Mixtures’ By Nmrmentioning

confidence: 99%

“…Of particular interest for dereplication is tandem MS/MS data, but new analytical techniques such as 2D microwave spectra may prove useful in the future. Dereplication is not only key for the human health research field, but will allow for creating repositories relevant for food and environmental studies . In turn, these libraries of known compounds will help to identify compounds present in a mixture, a task that should be made as automatic as possible.…”

Section: ‘Complex Mixtures’ By Nmrmentioning

confidence: 99%

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Complex mixtures by NMR and complex NMR for mixtures: experimental and publication challenges

Wist

2016

Magnetic Reson in Chemistry

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Untargeted strategies have changed the rules of the game in complex mixture analysis, introducing an amazing potential for medical and biological applications that is just starting to be tapped. But with great power come great challenges; although untargeted mixture analysis opens the road for many exciting possibilities, the road is still full of perils. On the one hand, this article highlights some of the difficulties that need to be sorted for mixture analysis by NMR to fulfill its potential, along with insight on how they may be managed. Highlighted key points include the need for 'computer friendly' solutions for sharing data, experimental design and algorithm to facilitate the steady growth of knowledge and modeling ability in the field, and the need for large-scale studies to improve confidence in newly identified biomarkers. On the other hand, the second part of this article presents some breakthroughs in NMR experiments that, when combined, may modify the landscape of mixture analysis. Copyright © 2016 John Wiley & Sons, Ltd.

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“…MS-based proteomics can effectively detect food allergens that are usually neglected by traditional nucleic acid detection or enzyme-linked immunosorbent assay (ELISA) based on antigen-antibody reactions. If the sample extraction process is optimized, traditional twodimensional electrophoresis and protein microarray techniques will also be able to detect allergens as precisely as at the parts per billion (ppb) level (Picariello et al,. 2013).…”

Section: Food Quality and Safety Researchmentioning

confidence: 99%

System Biological Research on Food Quality for Personalised Nutrition and Health Using Foodomics Techniques: A Review

Zheng¹,

Chen²

2014

JFNR

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Food quality is closely related to human health as people obtain energy from food. Traditional food science focuses on the provision of enough food for health guarantee. With economic development, people have a rising demand on quality and safety of the food they eat. Today, people are not only interested in eating sufficient and appropriate food, but expect to prevent diseases or treat existing diseases with diets. The development of the systems biology, especially various omics tools, is beneficial to the study of the impact of food compositions and ingredients on human health, advancing traditional food research into a new age: foodomics. Foodomics aims at studying the relationship between food quality and safety and food compositions and nutrition using omics tools such as genomics, transcriptomics, proteomics, and metabolomics, in addition to analytical techniques including chemometrics and bioinformatics. This review discusses recent advances in foodomics research.

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Proteomic‐based Techniques for the Characterization of Food Allergens

Cited by 10 publications

References 115 publications

Proteomics in food: Quality, safety, microbes, and allergens

Proteomics in food: Quality, safety, microbes, and allergens

Complex mixtures by NMR and complex NMR for mixtures: experimental and publication challenges

System Biological Research on Food Quality for Personalised Nutrition and Health Using Foodomics Techniques: A Review

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