Proteomic Changes between Male and Female Worms of the Polychaetous Annelid Neanthes arenaceodentata before and after Spawning

Chandramouli, Kondethimmanahalli H.; Ravasi, Timothy; Reish, Donald J.; Qian, Pei‐Yuan

doi:10.1371/journal.pone.0072990

Cited by 4 publications

(7 citation statements)

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“…Proteomics tools have been used to investigate protein expression dynamics during early development 8 , and to assess reproductive patterns in male and female N. arenaceodentata 9 . Previous studies are extended by proteomic changes in males occur through nine reproductive cycles.…”

Section: Discussionmentioning

confidence: 99%

“…These proteins showed varied expression patterns before and after spawning in N . arenaceodentata 8 9 . The up-regulation of several myosins and actins during reproduction may facilitate the increased body weight essential for muscular functions during mating suggesting that increased reproductive effort in males may be mediated by muscular and contractile proteins.…”

Section: Discussionmentioning

confidence: 99%

“…The polychaetous annelid species complex of Neanthes acuminata has a widespread distribution, with names depending on geographic location. For example, the species present in southern California, Baja California, Hawaii and the Marshall Islands is known as Neanthes arenaceodentata and has been the subject of considerable research in fields such as life history 1 , toxicology 2 , behavior 3 4 , vitellogensis 5 , feeding rate metabolism 6 , nervous system architecture 7 , proteomics 8 9 and longevity 10 . Polychaetes exhibit variable reproductive patterns including pairing in mucoid tubes, external brooding and hermaphrodism 11 .…”

mentioning

confidence: 99%

“…Proteomic studies with N. arenaceodentata have analyzed the changes in the transition from egg to larvae 8 and in males and females before and after fertilization 9 . Patterns of proteins and phosphoproteins were found to change as eggs become larvae 8 .…”

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confidence: 99%

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Proteomic Changes Associated with Successive Reproductive Periods in Male Polychaetous Neanthes arenaceodentata

Chandramouli

Reish

Zhang

et al. 2015

Sci Rep

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The polychaetous annelid Neanthes acuminata complex has a widespread distribution, with the California population referred to as N. arenaceodentata. The reproductive pattern in this complex is unique, in that the female reproduces once and then dies, whereas the male can reproduce up to nine times. The male incubates the embryos until the larvae leave the male’s tube 21–28 days later and commences feeding. Reproductive success and protein expression patterns were measured over the nine reproductive periods. The percent success of the male in producing juveniles increased during the first three reproductive periods and then decreased, but the number of juveniles produced was similar through all nine periods. iTRAQ based quantitative proteomics were used to analyze the dynamics of protein expression patterns. The expression patterns of several proteins were found to be altered. The abundant expression of muscular and contractile proteins may have affected body weight and reproductive success. Sperm have never been observed; fertilization occurs within the parent’s tube. Proteins associated with sperm maturation and fertilization were identified, including ATPase, clathrin, peroxiredoxins and enolase, which may provide clues to the molecular mechanisms enabling males to reproduce multiple times.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

mentioning

confidence: 99%

mentioning

confidence: 99%

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Proteomic Changes Associated with Successive Reproductive Periods in Male Polychaetous Neanthes arenaceodentata

Chandramouli

Reish

Zhang

et al. 2015

Sci Rep

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“…In addition, the up-regulation of glyceraldehyde-3-phosphate dehydrogenase probably implied that female mussels used extra glyceraldehyde-3-phosphate dehydrogenase to prevent them from apoptosis and oxidative stress induced by TBBPA exposure (Chandramouli et al, 2013).…”

Section: Metabolismmentioning

confidence: 99%

iTRAQ-based quantitative proteomic analyses on the gender-specific responses in mussel Mytilus galloprovincialis to tetrabromobisphenol A

Wei

et al. 2014

Aquatic Toxicology

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a b s t r a c tTetrabromobisphenol A (TBBPA) accounts for the largest production of brominated flame-retardants (BFRs) along the Laizhou Bay in China and is the most widely used BFR in industrial products. It can induce diverse toxicities including hepatotoxicity, nephrotoxicity, neurotoxicity and endocrine disrupting effects in mammalian and fish models. In this work, we applied iTRAQ-based proteomics to investigate the gender-specific responses in mussel Mytilus galloprovincialis to TBBPA. Thirty-one proteins were differentially expressed in hepatopancreas between male and female mussels, which clearly indicated the biological differences between male and female mussels at the protein level. After exposure of TBBPA (18.4 nmol/L) for one month, a total of 60 proteins were differentially expressed in response to the TBBPA treatment in mussel hepatopancreas, among which 33 and 29 proteins were significantly altered in TBBPA-treated male and female mussel samples, respectively. Only two of the 60 proteins were commonly altered in both male and female mussel samples exposed to TBBPA. Based on KEGG analysis, these differentially expressed proteins of TBBPA-induced effects were assigned to several groups, including cytoskeleton, reproduction and development, metabolism, signal transduction, gene expression, stress response and apoptosis. Overall, results indicated that TBBPA exposure could induce apoptosis, oxidative and immune stresses and disruption in energy, protein and lipid metabolisms in both male and female mussels with different mechanisms. This work suggested that the gender differences should be considered in ecotoxicoproteomics.

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Transcriptome and proteome dynamics in larvae of the barnacle Balanus Amphitrite from the Red Sea

et al. 2015

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BackgroundThe barnacle Balanus amphitrite is widely distributed in marine shallow and tidal waters, and has significant economic and ecological importance. Nauplii, the first larval stage of most crustaceans, are extremely abundant in the marine zooplankton. However, a lack of genome information has hindered elucidation of the molecular mechanisms of development, settlement and survival strategies in extreme marine environments. We sequenced and constructed the genome dataset for nauplii to obtain comprehensive larval genetic information. We also investigated iTRAQ-based protein expression patterns to reveal the molecular basis of nauplii development, and to gain information on larval survival strategies in the Red Sea marine environment.ResultsA nauplii larval transcript dataset, containing 92,117 predicted open reading frames (ORFs), was constructed and used as a reference for the proteome analysis. Genes related to translation, oxidative phosphorylation and cytoskeletal development were highly abundant. We observed remarkable plasticity in the proteome of Red Sea larvae. The proteins associated with development, stress responses and osmoregulation showed the most significant differences between the two larval populations studied. The synergistic overexpression of heat shock and osmoregulatory proteins may facilitate larval survival in intertidal habitats or in extreme environments.ConclusionsWe presented, for the first time, comprehensive transcriptome and proteome datasets for Red Sea nauplii. The datasets provide a foundation for future investigations focused on the survival mechanisms of other crustaceans in extreme marine environments.Electronic supplementary materialThe online version of this article (doi:10.1186/s12864-015-2262-1) contains supplementary material, which is available to authorized users.

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Proteomic Changes between Male and Female Worms of the Polychaetous Annelid Neanthes arenaceodentata before and after Spawning

Cited by 4 publications

References 40 publications

Proteomic Changes Associated with Successive Reproductive Periods in Male Polychaetous Neanthes arenaceodentata

Proteomic Changes Associated with Successive Reproductive Periods in Male Polychaetous Neanthes arenaceodentata

iTRAQ-based quantitative proteomic analyses on the gender-specific responses in mussel Mytilus galloprovincialis to tetrabromobisphenol A

Transcriptome and proteome dynamics in larvae of the barnacle Balanus Amphitrite from the Red Sea

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