Proteomic Changes during MCMV Infection Revealed by iTRAQ Quantitative Proteomic Analysis in Maize

Abstract

Maize chlorotic mottle virus (MCMV) has been occurring frequently worldwide and causes severe yield losses in maize (Zea mays). To better investigate the destructive effects of MCMV infection on maize plants, isobaric tagging for relative and absolute quantitation (iTRAQ)-based comparative proteomic analysis was performed on MCMV infected maize cv. B73. A total of 972 differentially abundant proteins (DAPs), including 661 proteins with increased abundance and 311 proteins with reduced abundance, were identified in response to MCMV infection. Functional annotations of DAPs and measurement of photosynthetic activity revealed that photosynthesis was decreased, while the abundance of ribosomal proteins, proteins related to stress responses, oxidation-reduction and redox homeostasis was altered significantly during MCMV infection. Two DAPs, disulfide isomerases like protein ZmPDIL-1 and peroxiredoxin family protein ZmPrx5, were further analyzed for their roles during MCMV infection through cucumber mosaic virus-based virus-induced gene silencing (CMV-VIGS). The accumulation of MCMV was suppressed in ZmPDIL-1-silenced or ZmPrx5-silenced B73 maize, suggesting ZmPDIL-1 and ZmPrx5 might enhance host susceptibility to MCMV infection.

Sugarcane Streak Mosaic Virus P1 Attenuates Plant Antiviral Immunity and Enhances Potato Virus X Infection in Nicotiana benthamiana

“…Owing to the low sample requirement, high accuracy, and wide proteome coverage, the 4D-proteomic approach has become one of the primary tools in functional genomics studies in different research fields [ 17 , 18 , 19 , 20 ]. In agricultural science, label-free peptide quantitation proteomics has been widely used to characterise plant responses to abiotic and biotic stresses, including stresses caused by plant virus infections [ 21 , 22 ]. Due to a lack of the infectious clone of SCSMV, the PVX-mediated overexpression system is well-characterised, with high protein expression levels, systemic infection, and persistence in N. benthamiana [ 23 , 24 ].…”

Comparative proteomic analysis of Nicotiana benthamiana plants under Chinese wheat mosaic virus infection

“…Maize cv. B73 plants infected with maize chlorotic mottle virus (MCMV) has been investigated in a comparative proteomic study to produce detailed whole-proteome information showing that the expression levels of ribosomal proteins, proteins related to stress responses, oxidation-reduction processes, and redox homeostasis are significantly altered during MCMV infection [ 26 ]. Nicotiana benthamiana is the most commonly experimental model in molecular plant-microbe interaction studies because it is highly susceptible to multiple pathogens and amenable to transient protein expression and virus-induced gene silencing [ 27 ].…”

Comparative Proteomic Analysis of Nicotiana Benthamiana Seedings Under Chinese Wheat Mosaic Virus Infection

“…Maize cv. B73 plants infected with maize chlorotic mottle virus (MCMV) has been investigated by comparative proteomic approach to get a detailed whole-proteome information that the expression levels of ribosomal proteins, proteins related to stress responses, oxidation-reduction and redox homeostasis are changed significantly under MCMV infection [26]. N. benthamiana is the most widely used experimental model in molecular plant-microbe interactions for it is very susceptible to multiple pathogens and amenable to transient protein expression manners and virus-induced gene silencing.…”