Proteomic characteristics of human sperm cryopreservation

Wang, Shangqian; Wang, Wei; Xu, Yang; Tang, Min; Fang, Jianzheng; Sun, Hongyong; Sun, Yangyang; Gu, Mei-Juan; Liu, Zhili; Zhang, Zhaoxia; Lin, Faxi; Wu, Ting; Song, Ninghong; Wang, Zengjun; Zhang, Wei; Chen, Yin

doi:10.1002/pmic.201300225

Cited by 125 publications

(102 citation statements)

References 33 publications

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“…It has shown a lower level of expression in human freeze-thawed spermatozoa compared with normal fresh semen. The motility of the latter was notably higher than the former [Wang et al 2014]. Therefore, it seems that there might be a relationship between the decrease of ACO2 and low sperm motility.…”

Section: Introductionmentioning

confidence: 88%

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The role of mitochondrial aconitate (ACO2) in human sperm motility

Tang

Liu

Wang

et al. 2014

Systems Biology in Reproductive Medicine

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Deficiencies in tricarboxylic acid (TCA) cycle enzymes have been shown to cause a wide spectrum of human diseases, including malignancies and neurological and cardiac diseases. In mammalian spermatozoa mitochondria, the TCA cycle is known to be a crucial metabolic pathway that contributes to produce ATP. There is little known about the role and mechanism of mitochondrial aconitase (ACO2), which is an important regulatory enzyme of the TCA cycle, in asthenozoospermia. In the current study, immunofluorescence staining localized ACO2 to the human sperm mid-piece. By immunoblotting, we demonstrated that the level of ACO2 protein in asthenozoospermic samples was significantly decreased compared with that in normal fertile men. Importantly, we first observed that co-incubation of isocitrate with low motile sperm suspensions significantly improved sperm motility, which might be due to elevated intracellular ATP. The improvement of the sperm motility by isocitrate may have important clinical implications in the treatment of asthenozoospermia and certainly warrants further investigation.

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Section: Introductionmentioning

confidence: 88%

“…Mitochondrial aconitase (ACO2) is involved in the TCA cycle and catalyzes the isomerization of citrate to isocitrate via cis-aconitate, which associates with ATP-dependent sperm motility [Beinert and Kennedy 1993;Wang et al 2014]. It has shown a lower level of expression in human freeze-thawed spermatozoa compared with normal fresh semen.…”

Section: Introductionmentioning

confidence: 99%

The role of mitochondrial aconitate (ACO2) in human sperm motility

Tang

Liu

Wang

et al. 2014

Systems Biology in Reproductive Medicine

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“…1 Since 2005, the next-generation sequencing (NGS) technologies 2 have allowed us to better understand the molecular classification of cancer, which may provide benefits for clinical practice in oncology, such as diagnosis, prognosis, and treatment decisions. Going along with proteomics in the clinical and biological applications, 3 The Cancer Genome Atlas (TCGA) has performed a lot of sequencing analysis, including whole exome sequencing, transcriptome profiling, and DNA methylation analysis. In prostate cancer, the TCGA was presented with a comprehensive molecular analysis of 333 primary prostate cancer, 4 which revealed a molecular taxonomy with seven different subtypes, such as specific gene fusions (erythroblast transformation specific (ETS) family of transcription factors), or mutations (speckletype POZ protein (SPOP), forkhead box protein A1 ALDH1A3 correlates with luminal phenotype in prostate cancer (FOXA1)).…”

Section: Introductionmentioning

confidence: 99%

ALDH1A3 correlates with luminal phenotype in prostate cancer

et al. 2017

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Prostate cancer is the most common male malignancies in the United States. The specific characteristics of different disease stages have been deeply investigated. We present our data on ALDH1A3 as a potential therapeutic target for the prostate cancer based on several functional investigations. Also, we used The Cancer Genome Atlas datasets for primary prostate cancer to detect the relevance of ALDH1A3 and prostate cancer luminal phenotype. We found that ALDH1A3 correlated with androgen receptor signaling pathway in primary prostate cancer, which is consistent with its luminal layer localization. Then, from the genetic manipulation assay, we knocked out the ALDH1A3 in PC-3 cells and found significantly reduced proliferation rate as well as the invasion ability. Furthermore, we looked up our single center primary prostate cancer post-operative follow-up data and suggested that the high level ALDH1A3 expression could predict the poor progression-free survival in a 158-patient cohort. We concluded that ALDH1A3, localized in luminal layer in prostate epithelium, is highly expressed in prostate cancer. It played important role in maintaining the proliferation, invasion, and cell cycle. It can also become the potential biomarker in the future to guide the therapeutic manipulations for primary prostate cancer.

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“…The liquefied semen samples were mixed with an equal volume of 10% glycerol–10% yolk freezing medium 10. The equilibrated samples were transferred to 2 ml cryovials (Greiner Bio-One GmbH, Frickenhausen, Germany) and a programmable freezer (Kry320-1.7, Planer PLC, Sunbury-on-Thames, UK) to obtain cooling from +20°C to −80°C.…”

Section: Methodsmentioning

confidence: 99%

Multivariate model for predicting semen cryopreservation outcomes in a human sperm bank

et al. 2017

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Semen cryopreservation is widely used in assisted reproductive technologies, but it reduces sperm quality dramatically. The aim of this study was to develop a model using basal semen quality to predict the outcome of postthaw semen parameters and improve the efficiency of cryopreservation in a human sperm bank. Basal semen parameters of 180 samples were evaluated in the first stage, and a multiple logistic regression analysis involving a backward elimination selection procedure was applied to select independent predictors. After a comprehensive analysis of all results, we developed a new model to assess the freezability of sperm. Progressive motility (PR), straight-line velocity (VSL) and average path velocity (VAP) were included in our model. A greater area under the receiver operating characteristic curve was obtained in our model when compared with other indicators. In the second stage of our study, samples that satisfied the new model were selected to undergo freeze–thawing. Compared with the first stage, the rate of good freezability was increased significantly (94% vs 67%, P = 0.003). By determining basal semen quality, we have developed a new model to improve the efficiency of cryopreservation in a human sperm bank.

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Proteomic characteristics of human sperm cryopreservation

Cited by 125 publications

References 33 publications

The role of mitochondrial aconitate (ACO2) in human sperm motility

The role of mitochondrial aconitate (ACO2) in human sperm motility

ALDH1A3 correlates with luminal phenotype in prostate cancer

Multivariate model for predicting semen cryopreservation outcomes in a human sperm bank

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