Proteomic characterization and comparison of Malaysian Bungarus candidus and Bungarus fasciatus venoms

Rusmili, Muhamad Rusdi Ahmad; Yee, Tee Ting; Mustafa, Mohd Rais; Hodgson, Wayne Clarence; Othman, Iekhsan

doi:10.1016/j.jprot.2014.08.001

Cited by 46 publications

(45 citation statements)

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“…Pre-synaptic neurotoxins similar to β-bungarotoxin are present in B. fasciatus venom [35]. However, such activity was not prominent in this study, even with the presence of TCAV.…”

Section: Discussioncontrasting

confidence: 57%

“…Interestingly, in the current study, TCAV effectively neutralised the post-synaptic neurotoxicity of all the venoms but failed to neutralise the pre-synaptic effects of B. caeruleus , B. fasciatus , O. scutellatus and N. scutatus venoms. These venoms all cause paralysis in humans and are known to contain pre-synaptic toxins [11,18,34,35,36]. …”

Section: Discussionmentioning

confidence: 99%

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Cross-Neutralisation of In Vitro Neurotoxicity of Asian and Australian Snake Neurotoxins and Venoms by Different Antivenoms

Silva

Hodgson

2016

Toxins

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There is limited information on the cross-neutralisation of neurotoxic venoms with antivenoms. Cross-neutralisation of the in vitro neurotoxicity of four Asian and four Australian snake venoms, four post-synaptic neurotoxins (α-bungarotoxin, α-elapitoxin-Nk2a, α-elapitoxin-Ppr1 and α-scutoxin; 100 nM) and one pre-synaptic neurotoxin (taipoxin; 100 nM) was studied with five antivenoms: Thai cobra antivenom (TCAV), death adder antivenom (DAAV), Thai neuro polyvalent antivenom (TNPAV), Indian Polyvalent antivenom (IPAV) and Australian polyvalent antivenom (APAV). The chick biventer cervicis nerve-muscle preparation was used for this study. Antivenom was added to the organ bath 20 min prior to venom. Pre- and post-synaptic neurotoxicity of Bungarus caeruleus and Bungarus fasciatus venoms was neutralised by all antivenoms except TCAV, which did not neutralise pre-synaptic activity. Post-synaptic neurotoxicity of Ophiophagus hannah was neutralised by all antivenoms, and Naja kaouthia by all antivenoms except IPAV. Pre- and post-synaptic neurotoxicity of Notechis scutatus was neutralised by all antivenoms, except TCAV, which only partially neutralised pre-synaptic activity. Pre- and post-synaptic neurotoxicity of Oxyuranus scutellatus was neutralised by TNPAV and APAV, but TCAV and IPAV only neutralised post-synaptic neurotoxicity. Post-synaptic neurotoxicity of Acanthophis antarcticus was neutralised by all antivenoms except IPAV. Pseudonaja textillis post-synaptic neurotoxicity was only neutralised by APAV. The α-neurotoxins were neutralised by TNPAV and APAV, and taipoxin by all antivenoms except IPAV. Antivenoms raised against venoms with post-synaptic neurotoxic activity (TCAV) cross-neutralised the post-synaptic activity of multiple snake venoms. Antivenoms raised against pre- and post-synaptic neurotoxic venoms (TNPAV, IPAV, APAV) cross-neutralised both activities of Asian and Australian venoms. While acknowledging the limitations of adding antivenom prior to venom in an in vitro preparation, cross-neutralization of neurotoxicity means that antivenoms from one region may be effective in other regions which do not have effective antivenoms. TCAV only neutralized post-synaptic neurotoxicity and is potentially useful in distinguishing pre-synaptic and post-synaptic effects in the chick biventer cervicis preparation.

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“…Pre-synaptic neurotoxins similar to β-bungarotoxin are present in B. fasciatus venom [35]. However, such activity was not prominent in this study, even with the presence of TCAV.…”

Section: Discussioncontrasting

confidence: 57%

Section: Discussionmentioning

confidence: 99%

Cross-Neutralisation of In Vitro Neurotoxicity of Asian and Australian Snake Neurotoxins and Venoms by Different Antivenoms

Silva

Hodgson

2016

Toxins

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“…Shotgun-proteomics and LC–MS/MS approaches have been used to characterize many other snake venom proteomes, including kraits and cobras [17,18]. …”

Section: Resultsmentioning

confidence: 99%

Proteomic Characterization and Comparison of Malaysian Tropidolaemus wagleri and Cryptelytrops purpureomaculatus Venom Using Shotgun-Proteomics

Abidin

Rajadurai

Chowdhury

et al. 2016

Toxins

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Tropidolaemus wagleri and Cryptelytrops purpureomaculatus are venomous pit viper species commonly found in Malaysia. Tandem mass spectrometry analysis of the crude venoms has detected different proteins in T. wagleri and C. purpureomaculatus. They were classified into 13 venom protein families consisting of enzymatic and nonenzymatic proteins. Enzymatic families detected in T. wagleri and C. purpureomaculatus venom were snake venom metalloproteinase, phospholipase A2, l-amino acid oxidase, serine proteases, 5′-nucleotidase, phosphodiesterase, and phospholipase B. In addition, glutaminyl cyclotransferase was detected in C. purpureomaculatus. C-type lectin-like proteins were common nonenzymatic components in both species. Waglerin was present and unique to T. wagleri—it was not in C. purpureomaculatus venom. In contrast, cysteine-rich secretory protein, bradykinin-potentiating peptide, and C-type natriuretic peptide were present in C. purpureomaculatus venom. Composition of the venom proteome of T. wagleri and C. purpureomaculatus provides useful information to guide production of effective antivenom and identification of proteins with potential therapeutic applications.

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“…Our knowledge on the proteomes of venom has advanced remarkably since then, shedding light on the improvement of snakebite management as well as drug discovery [26][27][28][29]. To date, the proteomes of a considerable number of medically important venomous snakes have been reported (for examples: [31][32][33][34][35][36]), however, there were limited studies published on the proteome of sea snake venoms [37][38].…”

Section: Accepted Manuscriptmentioning

confidence: 99%

“…The findings of such a simplified venom proteome for H. schistosus is comparable to that reported for two other hydrophiid species: Hydrophis cyanocinctus with only 2 protein families [37] and Pelamis platura with 7 protein families [38], investigated using the similar methodology of reverse-phase HPLC, SDS-PAGE and peptide sequencing. Based on the proteomic findings, the toxin diversity of H. schistosus venom appears rather limited compared to some of its terrestrial elapidic relatives, such as cobras [31,32,34,36], king cobra [44] and kraits [33], which venom proteomes exhibit well beyond 30 proteins classified into at least 10 protein families. In addition, like many hydrophiids, the venom of H. schistosus is usually injected in a small concentrated amount yet capable of producing severe toxicity [14,21,37].…”

Section: Venom Proteome Of Hydrophis Schistosus: a Minimalist's Profilementioning

confidence: 99%

Venomics of the beaked sea snake, Hydrophis schistosus: A minimalist toxin arsenal and its cross-neutralization by heterologous antivenoms

Tan

Lim

et al. 2015

Journal of Proteomics

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Proteomic characterization and comparison of Malaysian Bungarus candidus and Bungarus fasciatus venoms

Cited by 46 publications

References 50 publications

Cross-Neutralisation of In Vitro Neurotoxicity of Asian and Australian Snake Neurotoxins and Venoms by Different Antivenoms

Cross-Neutralisation of In Vitro Neurotoxicity of Asian and Australian Snake Neurotoxins and Venoms by Different Antivenoms

Proteomic Characterization and Comparison of Malaysian Tropidolaemus wagleri and Cryptelytrops purpureomaculatus Venom Using Shotgun-Proteomics

Venomics of the beaked sea snake, Hydrophis schistosus: A minimalist toxin arsenal and its cross-neutralization by heterologous antivenoms

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