Proteomic characterization of biogenesis and functions of matrix vesicles released from mineralizing human osteoblast-like cells

Thouverey, Cyril; Malinowska, Agata; Balcerzak, Marcin; Strzelecka‐Kiliszek, Agnieszka; Buchet, René; Dadlez, Michał; Pikula, Sławomir

doi:10.1016/j.jprot.2011.04.005

Cited by 99 publications

(101 citation statements)

References 69 publications

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“…The identified proteins are presented in Table 1 gelsolin (Kwiatkowski et al 1989;Thouverey et al 2011;Kim et al 2013) and actin cytoplasmic 1 (Sen et al 2015). LCMS/MS analysis also revealed the cellular and secreted proteins associated with bone homeostasis, such as peptidyl-prolyl cis-trans isomerase B (Pyott et al 2011) and lysozyme C (Siebert et al 1978;Briggs & Arinzeh 2014).…”

Section: Identification Of Proteins Adsorbed Onto the Blasted Acid-etmentioning

confidence: 98%

Proteome analysis of human serum proteins adsorbed onto different titanium surfaces used in dental implants

Romero‐Gavilán¹,

Araújo‐Gomes²,

Ródenas³

et al. 2016

Biofouling

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Titanium dental implants are commonly used due to their biocompatibility and biochemical properties; blasted acid-etched Ti is used more frequently than smooth Ti surfaces. In this study, physicochemical characterisation revealed important differences in roughness, chemical composition and hydrophilicity, but no differences were found in cellular in vitro studies (proliferation and mineralization). On the other hand, the deposition of proteins onto the implant surface might affect in vivo osseointegration. To test that hypothesis, protein layers formed on both surface type discs after incubation with human serum were analysed.Using mass spectrometry (LC/MS/MS), 139 proteins were identified, 31 of which were associated with bone metabolism. Interestingly, Apo E, antithrombin and protein C adsorbed mostly onto blasted and acid-etched Ti, whereas the proteins of the complement system (C3) were found predominantly on smooth Ti surfaces. These results suggest that physicochemical characteristics could be responsible for the differences observed in the adsorbed protein layer.

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Section: Identification Of Proteins Adsorbed Onto the Blasted Acid-etmentioning

confidence: 98%

Proteome analysis of human serum proteins adsorbed onto different titanium surfaces used in dental implants

Romero‐Gavilán¹,

Araújo‐Gomes²,

Ródenas³

et al. 2016

Biofouling

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“…Phosphate transporter 1 (PiT-1) helps to incorporate the P i generated in the perivesicular space into the MVs. MVs isolated from growth plate cartilage have a higher content of cholesterol (Chol), SM and phosphatidylserine (PS) (Thouverey et al 2009(Thouverey et al , 2011Wuthier and Lipscomb 2011) than do the plasma membranes, as well as longer fatty acids (Abdallah et al 2014). It is significant that MVs are enriched in SM as SM, via the enzymatic action of SMPD3, is converted to the PC to be used by PHOSPHO1 to produce P i intravesicularly.…”

Section: The Role Of Phosphatases In Mv-mediated Initiation Of Skeletmentioning

confidence: 99%

Biophysical aspects of biomineralization

et al. 2017

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During the process of endochondral bone formation, chondrocytes and osteoblasts mineralize their extracellular matrix (ECM) by promoting the synthesis of hydroxyapatite (HA) seed crystals in the sheltered interior of membranelimited matrix vesicles (MVs). Several lipid and proteins present in the membrane of the MVs mediate the interactions of MVs with the ECM and regulate the initial mineral deposition and posterior propagation. Among the proteins of MV membranes, ion transporters control the availability of phosphate and calcium needed for initial HA deposition. Phosphatases (orphan phosphatase 1, ectonucleotide pyrophosphatase/ phosphodiesterase 1 and tissue-nonspecific alkaline phosphatase) play a crucial role in controlling the inorganic pyrophosphate/inorganic phosphate ratio that allows MVmediated initiation of mineralization. The lipidic microenvironment can help in the nucleation process of first crystals and also plays a crucial physiological role in the function of MVassociated enzymes and transporters (type III sodiumdependent phosphate transporters, annexins and Na + /K + ATPase). The whole process is mediated and regulated by the action of several molecules and steps, which make the process complex and highly regulated. Liposomes and proteoliposomes, as models of biological membranes, facilitate the understanding of lipid-protein interactions with emphasis on the properties of physicochemical and biochemical processes. In this review, we discuss the use of proteoliposomes as multiple protein carrier systems intended to mimic the various functions of MVs during the initiation and propagation of mineral growth in the course of biomineralization. We focus on studies applying biophysical tools to characterize the biomimetic models in order to gain an understanding of the importance of lipid-protein and lipid-lipid interfaces throughout the process.Keywords Lipid microenvironment . Biomineralization . Matrix vesicles . Liposome . Proteoliposome . Hydroxyapatite Mineralization process and the biogenesis of matrix vesiclesMineralization of cartilage and bone occurs by a series of physicochemical and biochemical processes that together facilitate the deposition of calcium phosphate. The deposited calcium phosphate is subsequently converted into hydroxyapatite (HA) [Ca 10 (PO 4 ) 6 (OH) 2 ] in specific areas of the extracellular matrix (ECM). Various experiments have revealed the presence of HA crystals both inside and outside collagen fibrils in the ECM (McNally et al. 2012) and also within the

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“…While progress has been made in the study of MVs, (3,(7)(8)(9)(10). Data on the MVs protein component have been generated.…”

Section: Introductionmentioning

confidence: 99%

MVsCarta: A protein database of matrix vesicles to aid understanding of biomineralization

Cui

Luan

et al. 2015

BST

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Proteomic characterization of biogenesis and functions of matrix vesicles released from mineralizing human osteoblast-like cells

Cited by 99 publications

References 69 publications

Proteome analysis of human serum proteins adsorbed onto different titanium surfaces used in dental implants

Proteome analysis of human serum proteins adsorbed onto different titanium surfaces used in dental implants

Biophysical aspects of biomineralization

MVsCarta: A protein database of matrix vesicles to aid understanding of biomineralization

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