2016
DOI: 10.5582/bst.2016.01090
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Proteomic characterization of histone variants in the mouse testis by mass spectrometry-based top-down analysis

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Cited by 15 publications
(11 citation statements)
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“…For each of these protein species, the detailed information including spectrum index, retention time in minute, the precursor ion (isolation m/z , experimental m/z , theoretical m/z , z , IPMD in ppm, theoretical monoisotopic mass in Da), protein name, PTMs, number of matched product ions, P score, PTM score, and protein species score was provided in Table S1. It is worth mentioning that only 16 protein species were identified in the literature focusing on top‐down proteomics of histones from mouse …”
Section: Resultsmentioning
confidence: 99%
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“…For each of these protein species, the detailed information including spectrum index, retention time in minute, the precursor ion (isolation m/z , experimental m/z , theoretical m/z , z , IPMD in ppm, theoretical monoisotopic mass in Da), protein name, PTMs, number of matched product ions, P score, PTM score, and protein species score was provided in Table S1. It is worth mentioning that only 16 protein species were identified in the literature focusing on top‐down proteomics of histones from mouse …”
Section: Resultsmentioning
confidence: 99%
“…With the development of high‐resolution mass spectrometry, top‐down proteomics, which characterizes proteins at the intact level and has the strength of 100% sequence coverage, has been the method of choice for full characterization of combinatorial PTMs in core histones from various species . For mouse specifically, in 2010, the core histones from murine embryonic stem cells were separated using RPLC, analyzed by Orbitrap MS with ESI and ETD, searched by a combination of Xtract, Protein Prospector, and Fragment Assignment by Visual Assistance (FAVA); seven protein species were identified .…”
Section: Introductionmentioning
confidence: 99%
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“…1 ). In our past study, we had employed MS-based analysis for verification of each separated histone H3 variant [16] , [18] . Epididymis is a tube connected to the testis where sperms flow and get temporarily stored [5] .…”
Section: Resultsmentioning
confidence: 99%
“…Since the protein expression of Bub1 is maintained in MII oocytes40, it is possible that it persists in zygotes and phosphorylates TH2A T120 in the first mitosis. In addition, recent proteomic analyses of mouse testis found that the acetylation of TH2A at the N-terminus is also expected41.…”
Section: Discussionmentioning
confidence: 99%