2005
DOI: 10.1074/jbc.m412742200
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Proteomic Characterization of Messenger Ribonucleoprotein Complexes Bound to Nontranslated or Translated Poly(A) mRNAs in the Rat Cerebral Cortex

Abstract: Receptor-triggered control of local postsynaptic protein synthesis plays a crucial role for enabling long lasting changes in synaptic functions, but signaling pathways that link receptor stimulation with translational control remain poorly known. Among the putative regulatory factors are mRNA-binding proteins (messenger ribonucleoprotein, mRNP), which control the fate of cytosolic localized mRNAs. Based on the assumption that a subset of mRNA is maintained in an inactive state, mRNP-mRNA complexes were separat… Show more

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Cited by 41 publications
(49 citation statements)
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“…Evidence exists for the presence of ribosomes in the large nerve endings of the photoreceptor cells in squid optic lobes (Crispino et al1997;Martin et al 1998;Koenig and Giuditta, 1999), the structures that give rise to the synaptosomes that we have used here. On discontinuous sucrose gradients both p65 and p37 mostly accumulated in the 0.2/0.4 M sucrose fractions of the gradient (FIG 4B), consistent with their being part of an RNP 40S complex (Angenstein et al 2005). In the presence of magnesium ion a significant portion of p65, but not p37, sedimented through the higher density gradient into the pellet fraction (FIG 4B, +Mg).…”
supporting
confidence: 58%
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“…Evidence exists for the presence of ribosomes in the large nerve endings of the photoreceptor cells in squid optic lobes (Crispino et al1997;Martin et al 1998;Koenig and Giuditta, 1999), the structures that give rise to the synaptosomes that we have used here. On discontinuous sucrose gradients both p65 and p37 mostly accumulated in the 0.2/0.4 M sucrose fractions of the gradient (FIG 4B), consistent with their being part of an RNP 40S complex (Angenstein et al 2005). In the presence of magnesium ion a significant portion of p65, but not p37, sedimented through the higher density gradient into the pellet fraction (FIG 4B, +Mg).…”
supporting
confidence: 58%
“…Distinct sedimentation properties of p65 and p37 in a sucrose density gradient. To investigate potential diversity in functional properties between the monomeric and dimeric forms of hnRNPA/B-like protein 2, the high speed pellet fraction (P2) from lysed synaptosomes was applied to a discontinuous sucrose gradient (FIG4A) that is used in mammalian systems to separate 40S RNP particles, that accumulate in the less dense top layers of the gradient, from polyribosomes that accumulate in the more dense bottom layer (Angenstein et al 2005). Evidence exists for the presence of ribosomes in the large nerve endings of the photoreceptor cells in squid optic lobes (Crispino et al1997;Martin et al 1998;Koenig and Giuditta, 1999), the structures that give rise to the synaptosomes that we have used here.…”
mentioning
confidence: 99%
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“…38 It is found in messenger ribonucleoprotein particles (mRNPs) that also contain RNA-binding proteins like hnRNPK, PABP-1 Staufen, β tubulin and the motor protein dynein. 58 SG formation occurs in cells exposed to environmental stress and is usually induced by stalled preinitiation complexes accumulating due to phosphorylation of eukatyotic translation initiation factor 2α (eIF2α), which blocks translation initiation. SGs contain mRNAs associated with small ribosomal subunit and certain translation factors, and SG assembly is promoted by any one of the RNA-binding proteins, many of which are able to oligomerize.…”
Section: Discussionmentioning
confidence: 99%
“…The precise role of G3BP-1 is unclear, although it binds to a series of mRNAs that encode proteins regulating cell proliferation, namely c-Myc (23) and CDK7 and CDK9 (24). Interestingly, Caprin-1 has recently been identified as a component of RNA-protein complexes in the brain (25,26). Caprin-1 itself exhibits RGG motifs (M. David, unpublished observation), characteristic of RNA-binding proteins, in its C-terminal HR-2 domain.…”
Section: Discussionmentioning
confidence: 99%