Proteomic characterization of seeds from yellow lupin (Lupinus luteusL.)

Abstract

Yellow lupin (Lupinus luteus L.) is a legume crop containing a large amount of protein in its seeds. In this study, we constructed a seed-protein catalog to provide a foundation for further study of the seeds. A total of 736 proteins were identified in 341 2DE spots by nano-LC-MS/MS. Eight storage proteins were found as multiple spots in the 2DE gels. The 736 proteins correspond to 152 unique proteins as shown by UniRef50 clustering. Sixty-seven of the 152 proteins were associated with KEGG-defined pathways. Of the remaining proteins, 57 were classified according to a GO term. The functions of the remaining 28 proteins have yet to be determined. This is the first yellow lupin seed-protein catalog, and it contains considerably more data than previously reported for white lupin (L. albus L.).

Proteomic analysis of Lupinus angustifolius (var. Zeus and Bojar) and Lupinus luteus (var. Lord and Parys) seed proteins and their hydrolysates

“…The lupin seed proteomic studies typically involved seeds other than those of the L. luteus . Recently, Ogura et al . carried out a detailed proteomic characterisation of L. luteus seeds proteins.…”

“…The results allowed the creation of a catalogue of proteins involved in different pathways. Although the high number of identified proteins present even in small quantities was reported by Ogura et al ., the main spots present on the 2‐DE map corresponded to storage proteins fractions. Moreover, β‐conglutin was identified in 205 spots, whereas α‐conglutin in 65 spots .…”

“…Although the high number of identified proteins present even in small quantities was reported by Ogura et al ., the main spots present on the 2‐DE map corresponded to storage proteins fractions. Moreover, β‐conglutin was identified in 205 spots, whereas α‐conglutin in 65 spots . This fact additionally confirmed that lupin seed proteome contains mainly storage proteins.…”

“…18 The lupin seed proteomic studies typically involved seeds other than those of the L. luteus. Recently, Ogura et al 19 carried out a detailed proteomic characterisation of L. luteus seeds proteins. In total, 737 proteins were identified in 341 spots based on a classical proteomic approach (2-DE combined with mass spectrometry identification).…”

The health benefits of sweet lupin seed flours and isolated proteins

“…The protein, which may correspond to 35-40% of the dry weight (Sujak, Kotlarz, & Strobel, 2006), is mostly composed of albumins and globulins in an approximate 1 to 9 ratio (Duranti, Consonni, Magni, Sessa, & Scarafoni, 2008). The most abundant families are known as α-conglutin (legumins), β-conglutin (vicilins), γ-conglutin, and δ-conglutin (Magni, Scarafoni, Herndl, Sessa, Prinsi, Espen, et al, 2007;Ogura, Ogihara, Sunairi, Takeishi, Aizawa, Olivos-Trujillo, et al, 2014;E. Sirtori, Resta, Brambilla, Zacherl, & Arnoldi, 2010;Wait, Gianazza, Brambilla, Eberini, Morandi, Arnoldi, et al, 2005).…”