Proteomic Characterization of the Venom Of Five<i>Bombus (Thoracobombus)</i>Species

Barkan, Nezahat Pinar; Bayazit, Mustafa Bilal; Demiralp, Fatma Duygu Özel

doi:10.1101/193524

Cited by 5 publications

(2 citation statements)

References 42 publications

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“…Aminopeptidases remove one or more specific N-terminal residues from target proteins or peptides, and are common in venom of snakes 73,74 , in the venom of the predatory ant Pachycondyla striata 75 , in the venom of the velvet spider Stegodyphus mimosarum 76 and in the venom of the genus Thoracobombus (Hymenoptera) 77 , even if its function has not been understood yet. Mammalian aminopeptidases, which cleave brain angiotensin II to angiotensin III, are implicated in the control of arterial blood pressure 78 .…”

Section: Discussionmentioning

confidence: 99%

“…Carboxypeptidases cleave peptide bonds at the C-terminal of a protein 89 . Putative venom serine carboxypeptidases were found in the venom of the ants Odontomachus monticola 90 and Tetramorium bicarinatum 91 , the snake Crotalus durissus terrificus 92 , the Hymenoptera of the Apidae family 77 and in the venom gland of the ectoparasitoid Anisopteromalus calandrae 31 . A serine carboxypeptidase was also found in the venom of the endoparasitoids Psyttalia concolor 93 and T. nigriceps 18 .…”

Section: Protein Categorymentioning

confidence: 99%

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An integrated transcriptomic and proteomic approach to identify the main Torymus sinensis venom components

Scieuzo

Salvia

Franco

et al. 2021

Sci Rep

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During oviposition, ectoparasitoid wasps not only inject their eggs but also a complex mixture of proteins and peptides (venom) in order to regulate the host physiology to benefit their progeny. Although several endoparasitoid venom proteins have been identified, little is known about the components of ectoparasitoid venom. To characterize the protein composition of Torymus sinensis Kamijo (Hymenoptera: Torymidae) venom, we used an integrated transcriptomic and proteomic approach and identified 143 venom proteins. Moreover, focusing on venom gland transcriptome, we selected additional 52 transcripts encoding putative venom proteins. As in other parasitoid venoms, hydrolases, including proteases, phosphatases, esterases, and nucleases, constitute the most abundant families in T. sinensis venom, followed by protease inhibitors. These proteins are potentially involved in the complex parasitic syndrome, with different effects on the immune system, physiological processes and development of the host, and contribute to provide nutrients to the parasitoid progeny. Although additional in vivo studies are needed, initial findings offer important information about venom factors and their putative host effects, which are essential to ensure the success of parasitism.

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Section: Discussionmentioning

confidence: 99%

Section: Protein Categorymentioning

confidence: 99%

An integrated transcriptomic and proteomic approach to identify the main Torymus sinensis venom components

Scieuzo

Salvia

Franco

et al. 2021

Sci Rep

View full text Add to dashboard Cite

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Antioxidant Activity and Mechanism of Action of Amwaprin: A Protein in Honeybee (Apis mellifera) Venom

Kim,

Lee,

Jin

2024

Antioxidants

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Bee venom contains several bioactive components, including enzymatic and non-enzymatic proteins. There is increasing interest in the bioactive components of bee venom since they have exhibited various pharmacological effects. Recently, Apis mellifera waprin (Amwaprin) was identified as a novel protein in Apis mellifera (honeybee) venom and characterized as an antimicrobial agent. Herein, the novel biological function of Amwaprin as an antioxidant is described. In addition, the antioxidant effects of Amwaprin in mammalian cells were investigated. Amwaprin inhibited the growth of, oxidative stress-induced cytotoxicity, and inflammatory response in mammalian NIH-3T3 cells. Amwaprin decreased caspase-3 activity during oxidative stress and exhibited protective activity against oxidative stress-induced cell apoptosis in NIH-3T3 and insect Sf9 cells. The mechanism underlying the cell protective effect of Amwaprin against oxidative stress is due to its direct binding to the cell membrane. Furthermore, Amwaprin demonstrated radical-scavenging activity and protected against oxidative DNA damage. These results suggest that the antioxidant capacity of Amwaprin is attributed to the synergistic effects of its radical-scavenging action and cell shielding, indicating its novel role as an antioxidant agent.

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Alteration of Bumblebee Venom Composition toward Higher Elevation

Barkan

Chevalier

Pradervand

et al. 2019

Toxins

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Venomous animals use venom, a complex biofluid composed of unique mixtures of proteins and peptides, for either predation or defense. Bumblebees, which occur in various habitats due to their unique thermoregulatory properties, mainly use venom for defense. Herein, we conducted an exploratory analysis of the venom composition of a bumblebee species (Bombus pascuorum) along an elevation gradient in the western Swiss Alps using shot-gun proteomic approaches to assess whether their defense mechanism varies along the gradient. The gradient was characterized by high temperatures and low humidity at low elevations and low temperatures and high humidity at high elevations. Venom composition is changing along the elevation gradient, with proteomic variation in the abundances of pain-inducing and allergenic proteins. In particular, the abundance of phospholipase A 2 -like, the main component of bumblebee venom, gradually decreases toward higher elevation (lower temperature), suggesting venom alteration and thus a decrease in bumblebee defense towards harsher environments. Larger datasets may complement this study to validate the observed novel trends.

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Proteomic Characterization of the Venom Of FiveBombus (Thoracobombus)Species

Cited by 5 publications

References 42 publications

An integrated transcriptomic and proteomic approach to identify the main Torymus sinensis venom components

An integrated transcriptomic and proteomic approach to identify the main Torymus sinensis venom components

Antioxidant Activity and Mechanism of Action of Amwaprin: A Protein in Honeybee (Apis mellifera) Venom

Alteration of Bumblebee Venom Composition toward Higher Elevation

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