Proteomic characterization of two snake venoms: <i>Naja naja atra</i> and <i>Agkistrodon halys</i>

Li, Shuting; Wang, Jingqiang; Zhang, Xumin; Ren, Yan; Wang, Ning; Zhao, Kang; Chen, Xishu; Zhao, Caifeng; Li, Xiaolei; Shao, Jingjing; Yin, Jingjing; West, Matthew B.; Xu, Nuo; Liu, Siqi

doi:10.1042/bj20040354

Cited by 121 publications

(76 citation statements)

References 27 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Phospholipase A 2 in pit viper venom is responsible for cell membrane disruption (11). The predominance of enzymatic components in viperid venoms is strongly supported by other proteomics study as well (12)(13)(14).…”

Section: Discussionsupporting

confidence: 51%

Cytotoxicity of Southeast Asian snake venoms

Jamunaa¹,

Vejayan²,

Halijah³

et al. 2012

J. Venom. Anim. Toxins incl. Trop. Dis

View full text Add to dashboard Cite

Cytotoxicity of venoms from eleven medically important snakes found in Southeast Asia (Naja kaouthia, Naja siamensis, Naja sumatrana, Ophiophagus hannah, Bungarus candidus, Bungarus fasciatus, Enhydrina schistosa, Calloselasma rhodostoma, Trimeresurus purpureomaculatus and Tropidolaemus sumatranus) was determined, based on the MTS cytotoxicity assay, which determines the survival of viable cells in monolayer MDCK and Vero cell cultures upon exposure to the snake venoms. Snake venom toxicity was expressed as the venom dose that killed 50% of the cells (CTC 50 ) under the assay conditions. Venoms of C. rhodostoma (2.6 µg/mL, 1.4 µg/mL) and O. hannah were the most cytotoxic (3.8 µg/mL, 1.7 µg/mL) whereas N. siamensis venom showed the least cytotoxicity (51.9 µg/mL, 45.7 µg/mL) against Vero and MDCK cells, respectively. All the viper venoms showed higher cytotoxic potency towards both Vero and MDCK cell lines, in comparison to krait and cobra venoms. E. schistosa did not cause cytotoxicity towards MDCK or Vero cells at the tested concentrations. The cytotoxicity correlates well with the known differences in the composition of venoms from cobras, kraits, vipers and sea snakes.

show abstract

Section: Discussionsupporting

confidence: 51%

Cytotoxicity of Southeast Asian snake venoms

Jamunaa¹,

Vejayan²,

Halijah³

et al. 2012

J. Venom. Anim. Toxins incl. Trop. Dis

View full text Add to dashboard Cite

show abstract

“…This phenomenon can be due to proteins undergoing posttranslational modifications, such as glycosylation and phosphorylation in order to perform their molecular function (28). These protein forms are present as trains of spots in 2DE profile as they vary slightly in their pI and molecular masses (24).…”

Section: Discussionmentioning

confidence: 99%

“…These results agree with several studies regarding the biochemical composition of snake venom. Viperidae venoms are rich in enzymes that are typically observed in higher molecular mass regions (24). On the other hand, Elapidae venoms are known to possess a multitude of basic polypeptides, mainly neurotoxins and cardiotoxins (cytotoxin) that correspond to the lower range of molecular mass (13,25,26).…”

Section: Discussionmentioning

confidence: 99%

Protein profile analysis of Malaysian snake venoms by two-dimensional gel electrophoresis

Vejayan¹,

Yee²,

Ponnudurai³

et al. 2010

J. Venom. Anim. Toxins incl. Trop. Dis

View full text Add to dashboard Cite

Snake venoms comprise a highly complex mixture of proteins, which requires for their characterization the use of versatile two-dimensional electrophoresis techniques. In the present study, venoms obtained from eight snakes (Ophiophagus hannah, Naja kaouthia, Naja sumatrana, Bungarus fasciatus, Trimeresurus sumatranus, Tropidolaemus wagleri, Enhydrina schistosa and Calloselasma rhodostoma) commonly found in Malaysia were separated based on two independent properties, isoelectric point (pI) and molecular weight (MW). Many differences in snake venoms at the inter-family, inter-subfamily, inter-genus and inter-species levels were revealed. Notably, proteins from individuals of the Viperidae family -Trimeresurus sumatranus, Tropidolaemus wagleri and Calloselasma rhodostoma -were found to be numerous and scattered by the two-dimensional gel electrophoresis (2DE) specifically in regions between 37 and 100 kDa compared to the Elapidae venom proteins. The latter were clustered at the basic and lower molecular mass region (less than 20 kDa). Trains of spots were commonly observed, indicating that these proteins may be derived from post-translational modifications. Ophiophagus hannah (Elapidae) revealed a great amount of protein spots in the higher molecular mass range when compared to Enhydrina schistosa, Naja kaouthia, Naja sumatrana and Bungarus fasciatus. Overall 2DE showed large differences in the venom profile of each species, which might be employed as an ancillary tool to the identification of venomous snake species.

show abstract

“…including cardiotoxin, cobrotoxin, haemotoxin, and phospholipase A2. 4 Patients envenomed by a cobra experience varying degrees of neurotoxicity and cytotoxicity depending upon the proportions of the venom components. Due to evolution and geographical variations, different cobra species cause distinct clinical effects.…”

Section: Introductionmentioning

confidence: 99%

Can surgical need in patients with Naja atra (Taiwan or Chinese cobra) envenomation be predicted in the emergency department?

Wang

et al. 2016

Hong Kong Med J

View full text Add to dashboard Cite

Objectives:To investigate the clinical predictors and the aetiologies for surgery in patients with Naja atra (Taiwan or Chinese cobra) envenomation.Methods: This case series was conducted in the only tertiary care centre in eastern Taiwan. Patients who presented to the emergency department with Naja atra bite between January 2008 and September 2014 were included. Clinical information was collected and compared between surgical and non-surgical patients.Results: A total of 28 patients with Naja atra envenomation presented to the emergency department during the study period. Of these, 60.7% (n=17) required surgery. Necrotising fasciitis (76.5%) was the main finding in surgery. Comparisons between surgical and non-surgical patients showed skin ecchymosis (odds ratio=34.36; 95% confidence interval, 2.20-536.08; P=0.012) and a high total dose of antivenin (≥6 vials; odds ratio=14.59; 95% confidence interval, 1.10-192.72; P=0.042) to be the most significant predictors of surgery. The rate of bacterial isolation from the surgical wound was 88.2%. Morganella morganii (76.5%), Enterococcus faecalis (58.8%), and Bacteroides fragilis (29.4%) were the most common pathogens involved. Bacterial susceptibility testing indicated that combined broadspectrum antibiotics were needed to cover mixed aerobic and anaerobic bacterial infection.

show abstract

Proteomic characterization of two snake venoms: Naja naja atra and Agkistrodon halys

Cited by 121 publications

References 27 publications

Cytotoxicity of Southeast Asian snake venoms

Cytotoxicity of Southeast Asian snake venoms

Protein profile analysis of Malaysian snake venoms by two-dimensional gel electrophoresis

Can surgical need in patients with Naja atra (Taiwan or Chinese cobra) envenomation be predicted in the emergency department?

Contact Info

Product

Resources

About