Proteomic-driven biomarker discovery in gestational diabetes mellitus: A review

Singh, Apoorva; Subramani, Elavarasan; Ray, Chaitali Datta; Rapole, Srikanth; Chaudhury, Koel

doi:10.1016/j.jprot.2015.07.020

Cited by 19 publications

(19 citation statements)

References 63 publications

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“…However, the access to human tissues samples, such as placenta, during pregnancy for clinical analysis purposes is very limited. Therefore, more attainable biological samples, such as the blood, would represent a more suitable alternative for the study of MFSD2a and many other molecules that may be used as proteomic biomarkers for the clinical evaluation of gestational diabetes mellitus (35). In this regard, in the present study we have reported a lower level of MFSD2a protein expression in maternal blood of GDM patients (Figures 1A,B).…”

Section: Discussionsupporting

confidence: 46%

Child Head Circumference and Placental MFSD2a Expression Are Associated to the Level of MFSD2a in Maternal Blood During Pregnancy

et al. 2020

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Section: Discussionsupporting

confidence: 46%

Child Head Circumference and Placental MFSD2a Expression Are Associated to the Level of MFSD2a in Maternal Blood During Pregnancy

et al. 2020

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Section: Methodsmentioning

confidence: 99%

“…In this context, the international guidelines have established that, as targets of good metabolic control, values of fasting plasma glucose lower than 95 mg/dL, of 1-hour plasma glucose lower than 140 mg/dL, 2-hour plasma glucose lower than 120 mg/dL, and of HbA 1c lower than 6% to 6.5%, needs to be obtained in these women. 10 Most of the studies conducted to date on GDM have concentrated on serum and amniotic fluid, but some authors have recently underscored the importance of taking a proteomic approach 11 to seek new placental peptides in GDM too. To investigate on the possible changes in the placenta protein profile induced by GDM, it would be interesting to evaluate this profile before and after the GDM presence, but of course, this approach cannot be done.…”

Section: Ravnsborg Et Almentioning

confidence: 99%

“…After protein precipitation with the Protein precipitation kit (Calbiochem, Merck Millipore, Milan, Italy), samples were processed individually and analyzed using a label-free LC-MS E approach and a nanoACQUITY UPLC System coupled to a SYNAPT-MS mass spectrometer equipped with a TRIZAIC source, as described elsewhere. 11 A pool of all the samples was also created and run in quadruplicate to obtain reproducibility data. The data obtained for each placenta sample were processed with the Progenesis QI for proteomics software (version 2.0, http://www.nonlinear.com) to identify and quantify the differently-abundant proteins, comparing known healthy and GDM samples, as previously described.…”

Section: Label-free Lc-ms E Analysismentioning

confidence: 99%

“…Most of the studies conducted to date on GDM have concentrated on serum and amniotic fluid, but some authors have recently underscored the importance of taking a proteomic approach to seek new placental peptides in GDM too. To investigate on the possible changes in the placenta protein profile induced by GDM, it would be interesting to evaluate this profile before and after the GDM presence, but of course, this approach cannot be done.…”

Section: Introductionmentioning

confidence: 99%

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Is the placental proteome impaired in well‐controlled gestational diabetes?

et al. 2019

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In pregnancy complicated by gestational diabetes mellitus (GDM), the human placenta shows several pathological functional and structural changes, but the extent to which maternal glycemic control contributes to placental abnormalities remains unclear. The aim of this study was to profile and compare the proteome of placentas from healthy pregnant women and those with GDM, to investigate the placenta‐specific protein composition and possible changes of its function in presence of GDM. Quantitative proteomic analysis, based on LC‐MSE approach, revealed that higher (approximately 15% increase) levels of galectin 1 and collagen alpha‐1 XIV chain (although the difference regarding the latter was at the limit of significance) were present in GDM samples, while heat shock 70 kDa protein 1A/1B was less abundant in GDM placental tissue. These data seem to indicate that GDM, when well controlled, did not markedly affect the placental proteome.

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Effect of magnesium supplementation on pregnancy outcome in gestational diabetes mellitus patients: A meta‐analysis of randomized controlled trials

Rong

2022

Food Science & Nutrition

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Conflicting evidence exists regarding the effectiveness of magnesium supplementation during pregnancy in gestational diabetes mellitus (GDM) patients. This meta‐analysis examines the effect of magnesium on glycemic indices and metabolic status in GDM. We searched databases for randomized controlled trials (RCTs) conducted, and after applying inclusion and exclusion criteria, a total of four RCTs were considered eligible for the analysis. Outcome parameters included markers for glycemic control and metabolic status. A total of four RCTs with 198 participants (control = 99; magnesium supplemented = 99) were selected for the analysis. Magnesium supplementation resulted in a significant reduction in markers of glycemic control—fasting plasma glucose (standard mean difference (trueμ^) = −0.83; 95% CI: [−1.13, –0.54]; p‐value <.0001), and insulin levels (trueμ^ = −0.95; 95% CI: [−1.38, −0.52]; p‐value <.0001). Also, Mg intake resulted in altered oxidative stress markers TAC (trueμ^ = 1.09; 95% CI: [0.10, 2.07]; p‐value = .03) of the pregnant women. No significant effect on GSH and CRP levels was observed. This study provides evidence of the positive effects of magnesium intervention on insulin sensitivity and oxidative stress in GDM patients.

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Proteomic-driven biomarker discovery in gestational diabetes mellitus: A review

Cited by 19 publications

References 63 publications

Child Head Circumference and Placental MFSD2a Expression Are Associated to the Level of MFSD2a in Maternal Blood During Pregnancy

Child Head Circumference and Placental MFSD2a Expression Are Associated to the Level of MFSD2a in Maternal Blood During Pregnancy

Is the placental proteome impaired in well‐controlled gestational diabetes?

Effect of magnesium supplementation on pregnancy outcome in gestational diabetes mellitus patients: A meta‐analysis of randomized controlled trials

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