2014
DOI: 10.2478/jomb-2014-0022
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Proteomic Effects of the Coagulation Proteinase Thrombin on LX-2 Hepatic Stellate Cells

Abstract: SummaryBackground: The aim of this study was to characterize the effects of the coagulation proteinase thrombin on proteomic level in human hepatic stellate LX-2 cells. Methods: Proteomic analyses were performed using surfaceenhanced laser desorption/ionization-time of flight mass spectrometry (SELDI-TOF-MS). The protein profiles obtained from LX-2 cell lysates using strong anion exchanger Q10 ProteinChip arrays were statistically analyzed. Results: The peak intensities of 50 protein/peptide clusters were iden… Show more

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