2015
DOI: 10.1021/acs.jproteome.5b00353
|View full text |Cite
|
Sign up to set email alerts
|

Proteomic Evidence for Components of Spider Silk Synthesis from Black Widow Silk Glands and Fibers

Abstract: Spider silk research has largely focused on spidroins, proteins that are the primary components of spider silk fibers. Although a number of spidroins have been characterized, other types of proteins associated with silk synthesis are virtually unknown. Previous analyses of tissue-specific RNA-seq libraries identified 647 predicted genes that were differentially expressed in silk glands of the Western black widow, Latrodectus hesperus. Only ∼5% of these silk-gland specific transcripts (SSTs) encode spidroins; a… Show more

Help me understand this report

Search citation statements

Order By: Relevance

Paper Sections

Select...
1
1
1
1

Citation Types

6
70
0

Year Published

2016
2016
2023
2023

Publication Types

Select...
6
2

Relationship

1
7

Authors

Journals

citations
Cited by 53 publications
(76 citation statements)
references
References 50 publications
6
70
0
Order By: Relevance
“…Previous experimental findings have suggested that spidroin expression is not exclusively gland specific [50][51][52][53][54] . Our RNA sequencing studies also suggested broader patterns of spidroin transcript expression across silk glands ( Supplementary Fig.…”
Section: Expression Profiling Of Individual Spidroins Across Multiplementioning
confidence: 93%
See 2 more Smart Citations
“…Previous experimental findings have suggested that spidroin expression is not exclusively gland specific [50][51][52][53][54] . Our RNA sequencing studies also suggested broader patterns of spidroin transcript expression across silk glands ( Supplementary Fig.…”
Section: Expression Profiling Of Individual Spidroins Across Multiplementioning
confidence: 93%
“…To identify non-spidroin candidate genes potentially involved in silk production, we cataloged transcripts that were (i) highly expressed and/or (ii) uniquely expressed in N. clavipes silk glands, resulting in a list of 649 candidates from our RNA sequencing data ( Supplementary Fig. 12 and Supplementary Table 15); 183 of these genes exhibited homology to documented silk gland-specific transcripts [50][51][52][53][54] . The candidates included catalytic enzymes such as kinases, proteases, dehydrogenases, acetyltransferases, and synthases, many of which are active in eukaryotic secretory systems 57 .…”
Section: Extreme Spidroin Diversity and Evolutionary Originsmentioning
confidence: 99%
See 1 more Smart Citation
“…Major ampullate and minor ampullate silks are used for draglines and in the construction of prey‐capture webs. In orb‐web and cob‐web weaving spiders, the major and minor ampullate silk glands are dramatically different in size (Peters, ; Mullen, ; Vollrath, ; Jeffery et al ., ; Chaw et al ., ). However, in our silk gland dissections, the ampullate‐shaped glands of D. triton were not dramatically different in size or shape within an individual.…”
Section: Resultsmentioning
confidence: 97%
“…In a proteomic study, major ampullate spidroin was found in La. hesperus tubuliform glands, suggesting that major ampullate spidroin may contribute to egg case wrapping in black widows (Chaw et al, 2015). In Northern blot analysis, A. diadematus tubuliform gland mRNA was positive for major ampullate spidroin 1 (also known as ADF-2; Guerette et al, 1996).…”
Section: Silk Gene Expression In Tubuliform Silk Glandsmentioning
confidence: 99%