Proteomic features of potential tumor suppressor NESG1 in nasopharyngeal carcinoma

Abstract

We previously defined the recently revised NESG1 gene as a potential tumor suppressor in nasopharyngeal carcinoma (NPC). Here, we further used proteomics technology to globally examine NESG1-controlled proteins in NPC cells. Twenty-six proteins were found to be deregulated by NESG1 using proteomics analysis while enolase 1 (alpha) (ENO1), heat shock protein 90 kDa beta (Grp94), member 1 (HSP90B1), and cathepsin D (CTSD) proteins were differentially expressed by Western blot. Interestingly, a-enolase (ENO1), an overexpressed gene in NPC, was confirmed as a NESG1-regulated protein in NPC cells. Overexpressed ENO1 not only restored cell proliferation and cell-cycle progression, but also antagonized the regulation of NESG1 to cell-cycle regulators p21 and CCNA1 expression as well as induced the expression of C-Myc, pRB, and E2F1 in NESG1-ovexpressed NPC cells. Real-time PCR and immunohistochemistry analysis showed that NESG1 expression is negatively correlated with ENO1 expression in NPC tissues. Our observations suggest that ENO1 downregulation plays an important role in NESG1-induced growth inhibition of NPC cancer cells.

VPS33B negatively modulated by nicotine functions as a tumor suppressor in colorectal cancer

“…This gene was cloned and revised by our team in 1999 and 2005 . We studied its functions, including proliferation, migration, invasion and related pathways in NPC cells, and defined it as a potential tumor suppressor . We also confirmed that NESG1 inhibited the pathogenesis of human nonsmall cell lung cancers (NSCLCs) …”

microRNA-374a suppresses colon cancer progression by directly reducing CCND1 to inactivate the PI3K/AKT pathway

“…Colony formation assay was analyzed according to a previous description [29]. Experiments were performed three times.…”

The FOXK1-CCDC43 Axis Promotes the Invasion and Metastasis of Colorectal Cancer Cells

“…Previous studies have shown genetic or epigenetic alterations in several CCDC genes in human cancers, including CCDC19 (NESG1) in nasopharyngeal carcinoma [7], CCDC62 …”

“…To date, more than 140 genes contain the coiled-coil domain, but their functions are unknown in many cases (http://www.genecards.org/). Previous studies have shown that approximately 40 CCDC genes are associated with diseases, including various metabolic diseases [4], cancers [5][6][7], mitochondrial disease [8], epigenetic disease [9] and heart disease [10]. The abnormal expression of CCDC containing proteins in nasopharyngeal carcinoma [7], prostate cancer [5], pancreatic cancer [6], gastric cancer [9], breast cancer [11] and colorectal cancer (CRC).…”