Proteomic identification of differentially expressed proteins in Gossypium thurberi inoculated with cotton Verticillium dahliae

Zhao, Fu’an; Fang, Weiping; Xie, De‐Yu; Zhao, Yuanming; Tang, Zhongjie; Li, Wu; Nie, Lihong; Lv, Shuping

doi:10.1016/j.plantsci.2011.10.007

Cited by 33 publications

(22 citation statements)

References 32 publications

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“…Verticillium dahliae Kleb. is an asexual soil‐borne and xylem‐invading fungal pathogen that infects over 200 dicotyledonous species, including cotton (Meschke et al ., ; Zhao et al ., ; Dung et al ., ). Genetic improvements through conventional breeding or genetic engineering that utilise plant resistance genes are the most economic and effective means for managing Verticillium wilt (Fradin and Thomma, ).…”

Section: Introductionmentioning

confidence: 98%

Cotton polyamine oxidase is required for spermine and camalexin signalling in the defence response to Verticillium dahliae

et al. 2015

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SUMMARYVerticillium dahliae is a destructive, soil-borne fungal pathogen that causes vascular wilt disease in many economically important crops worldwide. A polyamine oxidase (PAO) gene was identified and cloned by screening suppression subtractive hybridisation and cDNA libraries of cotton genotypes tolerant to Verticillium wilt and was induced early and strongly by inoculation with V. dahliae and application of plant hormone. Recombinant cotton polyamine oxidase (GhPAO) was found to catalyse the conversion of spermine (Spm) to spermidine (Spd) in vitro. Constitutive expression of GhPAO in Arabidopsis thaliana produced improved resistance to V. dahliae and maintained putrescine, Spd and Spm at high levels. Hydrogen peroxide (H 2 O 2 ), salicylic acid and camalexin (a phytoalexin) levels were distinctly increased in GhPAO-overexpressing Arabidopsis plants during V. dahliae infection when compared with wild-type plants, and Spm and camalexin efficiently inhibited growth of V. dahliae in vitro. Spermine promoted the accumulation of camalexin by inducing the expression of mitogen-activated protein kinases and cytochrome P450 proteins in Arabidopsis and cotton plants. The three polyamines all showed higher accumulation in tolerant cotton cultivars than in susceptible cotton cultivars after inoculation with V. dahliae. GhPAO silencing in cotton significantly reduced the Spd level and increased the Spm level, leading to enhanced susceptibility to infection by V. dahliae, and the levels of H 2 O 2 and camalexin were distinctly lower in GhPAO-silenced cotton plants after V. dahliae infection. Together, these results suggest that GhPAO contributes to resistance of the plant against V. dahliae through the mediation of Spm and camalexin signalling.

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Section: Introductionmentioning

confidence: 98%

Cotton polyamine oxidase is required for spermine and camalexin signalling in the defence response to Verticillium dahliae

et al. 2015

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“…secretes various polysaccharide lyases able to degrade the host's primary cell wall and enable proliferation in the xylem [5]. The host response to infection has been widely characterized at both the transcript [6], [7], [8], [9], [10], [11] and the protein [12], [13], [14] levels. In tomato [15] and cotton [16] the presence of the immune receptor Ve confers resistance, and the corresponding virulence effector has been described in the pathogen [17].…”

Section: Introductionmentioning

confidence: 99%

Verticillium Suppression Is Associated with the Glucosinolate Composition of Arabidopsis thaliana Leaves

et al. 2013

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The soil-borne fungal pathogen Verticillium longisporum is able to penetrate the root of a number of plant species and spread systemically via the xylem. Fumigation of Verticillium contaminated soil with Brassica green manure is used as an environmentally friendly method for crop protection. Here we present a study focused on the potential role of glucosinolates and their breakdown products of the model plant Arabidopsis thaliana in suppressing growth of V. longisporum. For this purpose we analysed the glucosinolate composition of the leaves and roots of a set of 19 key accessions of A. thaliana. The effect of volatile glucosinolate hydrolysis products on the in vitro growth of the pathogen was tested by exposing the fungus to hydrated lyophilized plant tissue. Volatiles released from leaf tissue were more effective than from root tissue in suppressing mycelial growth of V. longisporum. The accessions varied in their efficacy, with the most effective suppressing mycelial growth by 90%. An analysis of glucosinolate profiles and their enzymatic degradation products revealed a correlation between fungal growth inhibition and the concentration of alkenyl glucosinolates, particularly 2-propenyl (2Prop) glucosinolate, respectively its hydrolysis products. Exposure of the fungus to purified 2Prop glucosinolate revealed that its suppressive activity was correlated with its concentration. Spiking of 2Prop glucosinolate to leaf material of one of the least effective A. thaliana accessions led to fungal growth suppression. It is suggested that much of the inhibitory effect observed for the tested accessions can be explained by the accumulation of 2Prop glucosinolate.

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“…Using RNA-Seq-dependent transcriptional analysis, a subset of genes participating in lignin metabolism was demonstrated to be very important in the resistance of cotton to V. dahliae (19). Additionally, defense-and stress-related proteins, such as pathogenesis-related proteins and proteins likely to be involved in the oxidative burst, sugars, ethylene signaling, and isoprenoid synthesis, have recently been suggested to be involved in cotton response to V. dahliae (25,26). Most of the candidate genes involving in disease resistance are isolated from transcriptomic analysis, whereas only few genes have been functionally characterized (27,28).…”

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confidence: 99%

Proteomic and Virus-induced Gene Silencing (VIGS) Analyses Reveal That Gossypol, Brassinosteroids, and Jasmonic acid Contribute to the Resistance of Cotton to Verticillium dahliae

Gao

Long

Zhu

et al. 2013

Molecular & Cellular Proteomics

263

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Verticillium wilt causes massive annual losses of cotton yield, but the mechanism of cotton resistance to Verticillium dahliae is complex and poorly understood. In this study, a comparative proteomic analysis was performed in resistant cotton (Gossypium barbadense cv7124) on infection with V. dahliae. A total of 188 differentially expressed proteins were identified by mass spectrometry (MALDI-TOF/TOF) analysis and could be classified into 17 biological processes based on Gene Ontology annotation. Most of these proteins were implicated in stimulus response, cellular processes and metabolic processes. Based on the proteomic analysis, several genes involved in secondary metabolism, reactive oxygen burst and phytohormone signaling pathways were identified for further physiological and molecular analysis. The roles of the corresponding genes were further characterized by employing virus-induced gene silencing (VIGS). Based on the results, we suggest that the production of gossypol is sufficient to affect the cotton resistance to V. dahliae. Silencing of GbCAD1, a key enzyme involving in gossypol biosynthesis, compromised cotton resistance to V. dahliae. Reactive oxygen species and salicylic acid signaling may be also implicated as regulators in cotton responsive to V. dahliae according to the analysis of GbSSI2, an important regulator in the crosstalk between salicylic acid and jasmonic acid signal pathways. Moreover, brassinosteroids and jasmonic acid signaling may play essential roles in the cotton disease resistance to V. dahliae. The brassinosteroids signaling was activated in cotton on inoculation with V. dahliae and the disease resistance of cotton was enhanced after exogenous application of brassinolide. Meanwhile, jasmonic acid signaling was also activated in cotton after inoculation with V. dahliae and brassinolide application. These data provide highlights in the molecular basis of cotton resistance to V. dahliae. Molecular & Cellular

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Proteomic identification of differentially expressed proteins in Gossypium thurberi inoculated with cotton Verticillium dahliae

Cited by 33 publications

References 32 publications

Cotton polyamine oxidase is required for spermine and camalexin signalling in the defence response to Verticillium dahliae

Cotton polyamine oxidase is required for spermine and camalexin signalling in the defence response to Verticillium dahliae

Verticillium Suppression Is Associated with the Glucosinolate Composition of Arabidopsis thaliana Leaves

Proteomic and Virus-induced Gene Silencing (VIGS) Analyses Reveal That Gossypol, Brassinosteroids, and Jasmonic acid Contribute to the Resistance of Cotton to Verticillium dahliae

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