Proteomic identification of serum proteins to induce osteoconductivity of hydroxyapatite

Abstract

We performed proteomic analysis of rat serum proteins adsorbed on hydroxyapatite (HAp) and α-alumina (α-Al2O3) in order to identify proteins that specifically adsorb onto HAp and control cellular responses. Proteins with either or both molecular weight of 22-32 kDa and computed isoelectric point of 5.0-5.5 were preferentially adsorbed on HAp. In total, 182 proteins were adsorbed on both HAp and α-Al2O3, of which 14 were highly enriched on HAp, whereas 68 were adsorbed only on HAp. Therefore, 82 (14+68) proteins were further evaluated by bioinformatics and literature-based analyses. We predicted that hepatocyte growth factor and angiopoietinlike protein 3 (ANGPTL3) are candidate proteins responsible for the osteoconductivity of HAp. Although ANGPTL3 promoted the attachment and spreading of MC3T3-E1 cells, it did not promote their proliferation and differentiation. Our results suggest that specific adsorption of ANGPTL3 on HAp induced osteoconductivity by enhancing the attachment and spreading of osteoblasts.

Self-Prepared Hyaluronic Acid/Alkaline Gelatin Composite with Nano-Hydroxyapatite and Bone Morphogenetic Protein for Cranial Bone Formation

“…Therefore, nHAp played dual roles as a protein carrier and as a slow releaser of retained protein. It is well known that nHAp absorbs both many positively and negatively charged proteins by counter-charged portions [ 43 ]. This protein could be a growth factor such as BMP.…”

Site‐specific photo‐crosslinking/cleavage for protein–protein interface identification reveals oligomeric assembly of lysosomal‐associated membrane protein type 2A in mammalian cells

“…Protein–protein interactions comprise parts of biological regulation systems. Recent years have shown major advances in technologies for identifying interacting proteins: EMAS (enzyme‐mediated activation of radical sources; Honke & Kotani, 2012 ; Kotani et al, 2008 ); APEX (engineered ascorbate peroxidase; Ambrogelly et al, 2007 ; James et al, 2019 ); BioID (proximity‐dependent biotin identification; Choi‐Rhee et al, 2004 ; Roux et al, 2012 ); AirID (ancestral BirA for proximity‐dependent biotin identification; Kido et al, 2020 ); and the osteoconductivity‐directed proteome (Wang et al, 2021 ). Once the target proteins are identified, the next goals will be to determine the ways they interact and to develop modification strategies, for potential therapeutic applications.…”