Proteomic investigation of brain, liver and intestine in high feed intake and low feed intake Chinook salmon (Oncorhynchus tshawytscha)

Esmaeili, Moha; Carter, Chris; Wilson, Richard; Walker, Seumas P.; Miller, Matthew R.; Bridle, Andrew R.; Symonds, Jane E.

doi:10.1016/j.aquaculture.2022.737915

Cited by 12 publications

(3 citation statements)

References 86 publications

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“…For example, the optimum dietary lipid content in blunt snout bream ( Megalobrama amblycephala ) [ 34 ], tropical gar ( Atractosteus tropicus ) [ 35 ], and orange-spotted grouper ( Epinephelus coioides ) [ 36 ] was 100 g/kg diet, and that in golden pompano ( Trachinotus ovatus ) was 120 g/kg [ 37 ]. However, the optimum dietary lipid levels in salmonids diets were 230 g/kg for rainbow trout [ 38 ], 240 g/kg for Coho Salmon ( Oncorhynchus kisutch ) [ 39 ], 260 g/kg for triploid brown trout ( Salmo trutta ) [ 40 ], 231 g/kg for Chinook salmon ( Oncorhynchus tshawytscha ) (Ridley Corporation) [ 41 , 42 ], and 280 g/kg for Atlantic salmon [ 43 ]. This information shows that oscar is closer to carnivorous freshwater species such as salmonids.…”

Section: Resultsmentioning

confidence: 99%

Early Mild Stress along with Lipid Improves the Stress Responsiveness of Oscar (Astronotus ocellatus)

Esmaeili

Zare

Akhavan

et al. 2022

Aquaculture Nutrition

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Early-life exposure to mild stressors can assist animals in coping with more stressful events in later life. This study was aimed at investigating how early stress and dietary lipid contents affect growth, hematology, blood biochemistry, immunological responses, antioxidant system, liver enzymes, and stress responses of oscar (Astronotus ocellatus) ( 6.8 ± 0.7 g). Six experimental treatments were HL0Stress (high-lipid diet and without stress), HL2Stresses (high-lipid diet and two-week stress), HL4Stresses (high-lipid diet and four-week stress), LL0Stress (low-lipid diet and without stress), LL2Stresses (low-lipid diet and two-week stress), and LL4Stresses (low-lipid diet and four-week stress). During the ten-week trial, fish fed high-lipid diets grew faster ( 46.41 ± 4.67 vs. 38.81 ± 2.81 ) and had a lower feed conversion ratio (2.21 vs. 2.60) than those fed low-lipid diets ( P < 0.05 ). After acute confinement stress (AC stress), high-lipid groups had higher survival than low-lipid treatments (81.25% vs 72.92%) ( P < 0.05 ). Fish subjected to two-time stress (2Stresses) had a higher survival rate after AC stress (90.63% vs. 62.50%), hematocrit, white blood cell, blood performance, total protein, high-density lipoproteins, cholesterol, triglyceride, alternative complement activity (ACH50), superoxide dismutase, glutathione peroxidase, and alkaline phosphatase levels than those not stressed ( P < 0.05 ). Contrariwise, glucose, cortisol, alanine aminotransferase, and aspartate aminotransferase levels were significantly lower in the 2Stresses groups compared with 0Stress fish ( P < 0.05 ). Collectively, these findings suggest stressing the signs of adaptation in 2Stresses fish. However, a higher number of early stress events (4Stresses) appears to exceed the threshold of manageable stress levels for this species. In conclusion, the HL2Stresses group outperformed the other treatments in terms of growth, health status, and stress responsiveness. Although fish welfare must be considered, these results suggest that early mild stress can result in a greater survival rate after fish are exposed to later acute stress.

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Section: Resultsmentioning

confidence: 99%

Early Mild Stress along with Lipid Improves the Stress Responsiveness of Oscar (Astronotus ocellatus)

Esmaeili

Zare

Akhavan

et al. 2022

Aquaculture Nutrition

Self Cite

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“…Some other biological variations, such as the amount of feed intake, metabolism, and genetic factors, may have an influence on the weight of the individual. 46,47,48 Additionally, physical changes, including mortality, salivation, diarrhea, changes in feces color, and other abnormal behavioral responses, were observed to ascertain the toxicity effect of the plant extract. There were zero deaths recorded among all the experimental treatment groups, no sign of physical changes, or any abnormal behavioral response.…”

Section: Resultsmentioning

confidence: 99%

Pain Alleviating Potential of Ethanol Extract of Vernonia amygdalina Del. on Writhing in Male Wistar Rats

2024

TJNPR

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Pain, as described by the International Association for the Study of Pain (IASP), can be referred to as an experience of sensory and emotional distress that is linked with a potential or real tissue damage. 1,2 Pain can be localized, as in an injury, or it can be diffuse, as in illnesses such as fibromyalgia. According to Craig and MacKenzie, 3 pain is a complex experience with sensory, physiological, cognitive, emotional, and behavioral components. By altering the way noxious stimuli are transmitted to the brain, emotions, behavioral responses to pain, beliefs, and attitudes influence the way pain is felt. Pain is classified as nociceptive, which is an acute reaction to a mechanical insult or noxious stimuli; inflammatory, which is related to damage to the tissue and immune cell intrusion; and pathological (neuropathic pain) as a result of nervous system damage. 4 Pain may result in a decreased quality of life and an increased health expenditure. 5 Analgesics is any member of the group of medications that reduce or completely eliminate the pain associated with many pathologic conditions. 6,7 The mechanism of their actions is by interrupting signals caused by pain, from reaching the brain or by impeding the way the brain interprets the signals it receives. 8,9

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“…Providing further mechanistic insight into FE in farmed salmon, we have applied exploratory metabolomic- and proteomic-based approaches to screen for metabolite and protein biomarkers associated with divergent FE phenotypes. Using secondary bioinformatics techniques to reveal functional biochemical pathway information, findings highlighted that fish with high FE display greater capacity for protein synthesis in white muscle tissue, intestine, and liver, and increased energy production from elevated rates of lipid metabolism in the liver and brain [ 19 , 20 , 21 ]. Conversely, salmon with low FE displayed signatures indicative of protein degradation process (proteolysis and amino acid catabolism) being prominent in muscle, liver, and the intestine [ 21 , 22 ].…”

Section: Introductionmentioning

confidence: 99%

Prediction of Feed Efficiency and Performance-Based Traits in Fish via Integration of Multiple Omics and Clinical Covariates

Young

Laroche

Walker

et al. 2023

Biology

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Fish aquaculture is a rapidly expanding global industry, set to support growing demands for sources of marine protein. Enhancing feed efficiency (FE) in farmed fish is required to reduce production costs and improve sector sustainability. Recognising that organisms are complex systems whose emerging phenotypes are the product of multiple interacting molecular processes, systems-based approaches are expected to deliver new biological insights into FE and growth performance. Here, we establish 14 diverse layers of multi-omics and clinical covariates to assess their capacities to predict FE and associated performance traits in a fish model (Oncorhynchus tshawytscha) and uncover the influential variables. Inter-omic relatedness between the different layers revealed several significant concordances, particularly between datasets originating from similar material/tissue and between blood indicators and some of the proteomic (liver), metabolomic (liver), and microbiomic layers. Single- and multi-layer random forest (RF) regression models showed that integration of all data layers provide greater FE prediction power than any single-layer model alone. Although FE was among the most challenging of the traits we attempted to predict, the mean accuracy of 40 different FE models in terms of root-mean square errors normalized to percentage was 30.4%, supporting RF as a feature selection tool and approach for complex trait prediction. Major contributions to the integrated FE models were derived from layers of proteomic and metabolomic data, with substantial influence also provided by the lipid composition layer. A correlation matrix of the top 27 variables in the models highlighted FE trait-associations with faecal bacteria (Serratia spp.), palmitic and nervonic acid moieties in whole body lipids, levels of free glycerol in muscle, and N-acetylglutamic acid content in liver. In summary, we identified subsets of molecular characteristics for the assessment of commercially relevant performance-based metrics in farmed Chinook salmon.

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Proteomic investigation of brain, liver and intestine in high feed intake and low feed intake Chinook salmon (Oncorhynchus tshawytscha)

Cited by 12 publications

References 86 publications

Early Mild Stress along with Lipid Improves the Stress Responsiveness of Oscar (Astronotus ocellatus)

Early Mild Stress along with Lipid Improves the Stress Responsiveness of Oscar (Astronotus ocellatus)

Pain Alleviating Potential of Ethanol Extract of Vernonia amygdalina Del. on Writhing in Male Wistar Rats

Prediction of Feed Efficiency and Performance-Based Traits in Fish via Integration of Multiple Omics and Clinical Covariates

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