Proteomic Investigations of In Vitro and In Vivo Models of Periodontal Disease

Abstract

Proteomics has currently been a developing field in periodontal diseases to obtain protein information of certain samples. Periodontal disease is an inflammatory disorder that attacks the teeth, connective tissues, and alveolar bone within the oral cavity. Proteomics information can provide proteins that are differentially expressed in diseased or healthy samples. This review provides insight into approaches researching single species, multi species, bacteria, non-human, and human models of periodontal disease for proteomics information. The approaches that have been taken include gel electrophoresis and qualitative and quantitative mass spectrometry. This review is carried out by extracting information about in vitro and in vivo studies of proteomics in models of periodontal diseases that have been carried out in the past two decades. The research has concentrated on a relatively small but well-known group of microorganisms. A wide range of models has been reviewed and conclusions across the breadth of these studies are presented in this review.

The Role of the Oral Microbiota Related to Periodontal Diseases in Anxiety, Mood and Trauma- and Stress-Related Disorders

“…At the opposite end we can find the in vitro models in which cultures of different cell types are exposed to single species of bacteria classically related to periodontal diseases such as P. gingivalis or Fusobacterium nucleatum (F. nucleatum); or in combination (trying to resemble the situation of the biofilm in periodontitis patients). These in vitro models are typically used to evaluate and identify putative molecular/cellular mechanisms that could be explored in relation to behavioral alterations in subsequent in vivo models (73). Another interesting approach is the development of in vitro periodontal biofilm models to test the antimicrobial/anti-inflammatory profile of different compounds and therapeutic strategies (74)(75)(76).…”

Recent advances in the detection of glioblastoma, from imaging-based methods to proteomics and biosensors: A narrative review

“…Proteomics approaches can be readily employed to elucidate the natural production mechanisms of microorganisms and plants [ 57 , 58 ]. This strategy has also been successfully applied to different types of diseases, such as periodontitis, Alzheimer’s disease, thyroid disease, and various types of tumors [ 59 , 60 ]. In glioma, proteomics techniques have identified changes in protein expression, but the consistency and biological significance of these changes have not been established [ 61 ].…”

Evaluation of Dental Materials and Oral Disease-Related Proteins in Dentistry: Efficacy of Electrophoresis as a Valuable Tool

“…Electrophoresis analysis aids in the identification and characterization of biomarkers associated with periodontal diseases. This analysis allows for the identification of disease-specific proteins or protein patterns that can serve as potential biomarkers for periodontal diseases [51,52]. Here are some example proteins and genes that have been studied as potential biomarkers for periodontal disease:…”