Proteomic Landscape of Exosomes Reveals the Functional Contributions of CD151 in Triple-Negative Breast Cancer

Abstract

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Extracellular Vesicles: The Landscape in the Progression, Diagnosis, and Treatment of Triple-Negative Breast Cancer

“…In this process, the ASPH-Notch axis helped to prepare MMPs/ADAMs for exosomes synthesis/release, which was conducive to potentiate multifaceted metastasis. Sipeng Li et al (2021) identified that the expression level of CD151 in TNBC-derived serum exosomes was markedly higher than that in healthy subject exosomes. Using quantitative proteomics methods, they found that exosome CD151 promoted ribosomal protein secretion through exosomes and restrained exosome complement protein secretion.…”

Applications of Extracellular Vesicles in Triple-Negative Breast Cancer

“…For example, UCHL1 (ubiquitin carboxyl-terminal hydrolase isozyme L1) harboured in TNBC-TEVs has been proposed as a biomarker for TNBC [ 121 ]. It is also applicable to use surface membrane proteins (e.g., CD151) present on TNBC-TEVs as biomarkers for diagnosing TNBC [ 122 ]. Additional examples of potential protein biomarkers for TNBC-TEVs include the epidermal growth factor receptor (EGFR) and survivin [ 123 , 124 , 127 ].…”

One-Pot Exosome Proteomics Enabled by a Photocleavable Surfactant

“…Using this Azo-enabled method, exosome extraction and sample preparation require only ~2.5 h, compared to traditional methods employing overnight digestion and/or lengthy prefractionation that may take 16 to 24 h total. [15][16][17][18][19][20] The subsequent LC-TIMS-MS/MS analysis enhanced by PASEF drastically increases sensitivity without increasing analysis time, obviating the need for multidimensional LC or prefractionation, and increasing the total number of proteins identified in the extraction. 24,25 We have applied this method to analyze mammary fibroblast-derived exosomes.…”

“…7 Mass spectrometry (MS)-based proteomics is one of the most promising techniques for the global identification and quantification of proteins, [8][9][10] and has recently been employed to characterize exosomal protein cargoes. 1,7,[11][12][13][14][15][16][17][18][19][20] Typically, bottom-up proteomic methods are used in these MS-based analyses of EVs, but the sample preparation typically involves the use of MSincompatible detergents for protein extraction, overnight and/or multiple enzymatic digestions, and lengthy multidimensional chromatographic separations. [15][16][17][18][19][20] The elongated experimental time and complexity required to improve exosomal proteome coverage in these previously established methods significantly reduce the throughput and reproducibility, limiting the potential of MSbased proteomic analysis of exosomes in translational and clinical applications.…”