Proteomic Markers in the Muscles and Brain of Pigs Recovered from Hemorrhagic Stroke

Fedulova, Liliya; Vasilevskaya, Ekaterina; Tikhonova, Olga V.; Kazieva, L.Sh.; Tolmacheva, G. S.; Makarenko, Alexandr

doi:10.3390/genes13122204

Cited by 4 publications

(1 citation statement)

References 34 publications

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“…Omics technologies make it possible to obtain multiple genome-wide data arrays at a time and carry out multiple results comparisons in the conditions of one experiment. Studies based on transcriptomic and proteomic approaches can reveal the expression profiles of RNAs (RNA-Seq, single-cell RNA-Seq), proteins, and peptides to assess how much neuropathology has distorted biomolecule levels [122,124,[127][128][129][130][131]. At the same time, the normalization (compensation) of the disturbed profiles of the analyzed molecules after exposure to the peptide is evidence of the potential drug (peptide)'s neuroprotective properties under neuropathological conditions.…”

Section: Discussionmentioning

confidence: 99%

Neuroprotective Peptides and New Strategies for Ischemic Stroke Drug Discoveries

Dergunova

Filippenkov

Limborska

et al. 2023

Genes

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Ischemic stroke continues to be one of the leading causes of death and disability in the adult population worldwide. The currently used pharmacological methods for the treatment of ischemic stroke are not effective enough and require the search for new tools and approaches to identify therapeutic targets and potential neuroprotectors. Today, in the development of neuroprotective drugs for the treatment of stroke, special attention is paid to peptides. Namely, peptide action is aimed at blocking the cascade of pathological processes caused by a decrease in blood flow to the brain tissues. Different groups of peptides have therapeutic potential in ischemia. Among them are small interfering peptides that block protein–protein interactions, cationic arginine-rich peptides with a combination of various neuroprotective properties, shuttle peptides that ensure the permeability of neuroprotectors through the blood–brain barrier, and synthetic peptides that mimic natural regulatory peptides and hormones. In this review, we consider the latest achievements and trends in the development of new biologically active peptides, as well as the role of transcriptomic analysis in identifying the molecular mechanisms of action of potential drugs aimed at the treatment of ischemic stroke.

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Section: Discussionmentioning

confidence: 99%

Neuroprotective Peptides and New Strategies for Ischemic Stroke Drug Discoveries

Dergunova

Filippenkov

Limborska

et al. 2023

Genes

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Investigating recovery after a spontaneous intracerebral haemorrhage in zebrafish larvae

Crilly,

Shand,

Bennington

et al. 2024

Brain Communications

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Intracerebral haemorrhage is a debilitating stroke subtype with high morbidity and mortality rates. For survivors, rehabilitation is a long process, and with no available therapeutics to limit the immediate pathophysiology of the haemorrhage, recovery is dependent on individual neuroplasticity. We have previously shown that zebrafish larvae can be used to model spontaneous brain haemorrhage. Zebrafish exhibit innate recovery mechanisms and are often used as a model system for investigation into regeneration after injury, including injury to the nervous system. Here, we investigate the spontaneous and immediate recovery in zebrafish larvae following an intracerebral haemorrhage at 2 days post fertilisation, during pre-protected stages and over the first 3 weeks of life. We have shown that following the onset of bleed at ∼2 days post fertilisation zebrafish are capable of clearing the haematoma through the ventricles. Brain cell damage associated with intracerebral haemorrhage is resolved within 48 hours, and this recovery is associated with survival rates equal to wildtype and non-haemorrhaged sibling control animals. Larvae express more nestin-positive neural progenitor cells 24 hours after injury when the most damage is observed, and through mass spectrometry analysis we have determined that these cells are highly proliferative and may specially differentiate into oligodendrocytes. This study provides an insight into the haematoma resolution processes in a live, intact organism, and may suggest potential therapeutic approaches to support the recovery of intracerebral haemorrhage patients.

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Influence of pH on protein extraction from Sus scrofa pancreas

Polishchuk,

Aryzina,

Spirina

et al. 2024

Food systems

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The porcine pancreas contains various enzymes, structural, regulatory, secretory, receptor and other biologically active substances that ensure both the functioning of the organ and its biological role in the organism. The aim of this work was to study the influence of pH changes in 0.9% sodium chloride solution used as an extractant on the efficiency of bioactive protein isolation from the porcine pancreas. The extraction was carried out with the 0.9% NaCl, 0.9% NaCl pH=4 and 0.9% NaCl pH= 8.5 with a stirring speed of 400 rpm for 150 min at 4 ºC; the ratio of pancreas: extractant was 1:5, the supernatant was separated by centrifugation. The protein concentration was measured by a biuret reaction on a semi-automatic biochemical analyzer Biochem SA. The proteomic composition of extracts and native pancreas was evaluated by 10% SDS-PAGE according to Laemmli method in the “VE10” chamber. Digital images of electrophoregrams were obtained using a Bio-5000 Plus scanner, edited in a graphic editor and analyzed using ImageJ software. When determining the intensity of protein fractions, it was noted that the use of 0.9% NaCl contributed to a greater yield of proteins with molecular weights of 200 kDa, 150 kDa, 69 kDa, 52 kDa and 33 kDa into the extractant; a pH shift to the acidic area stimulated the yield of fractions with molecular weights of 130 kDa, 50 kDa, 49 kDa, 45 kDa, 40 kDa, 30 kDa and 27kDa, and a pH shift to the alkaline area — only 47 kDa and 42 kDa. Most pancreas proteolytic enzymes have a molecular weight in a range of 34–23kDa, excepting the immature form of carboxypeptidases with MW 45–47kDa. The greatest intensity of protein bands was observed in the region with MW less than 33kDa on the obtained electrophoregrams. The presence of intense protein fractions in the region of molecular weights of less than 50–52kDa and 40kDa was also noted, which may correspond to enzymes such as pancreatic lipase and phospholipase A2, and the presence of protein fractions with MW above 130 kDa corresponding to various types and isoforms of collagen and laminin. In addition, such processes as protein aggregation and proteolysis can also influence the molecular weight distribution of protein fractions.

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Proteomic Markers in the Muscles and Brain of Pigs Recovered from Hemorrhagic Stroke

Cited by 4 publications

References 34 publications

Neuroprotective Peptides and New Strategies for Ischemic Stroke Drug Discoveries

Neuroprotective Peptides and New Strategies for Ischemic Stroke Drug Discoveries

Investigating recovery after a spontaneous intracerebral haemorrhage in zebrafish larvae

Influence of pH on protein extraction from Sus scrofa pancreas

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