Proteomic profile of saliva in patients with Parkinson's disease after the practice of interval exercise

Ventura, Talita Mendes Oliveira; Santos, Karina Oliveira; Braga, Aline Silva; Grizzo, Larissa Tercília; Buzalaf, Marília Afonso Rabelo; Kalva-Filho, Carlos Augusto; Faria, Murilo Henrique; Barbieri, Fábio Augusto; Magalhães, Ana Carolina

doi:10.1016/j.parkreldis.2022.04.012

Cited by 1 publication

(9 citation statements)

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“…Data acquisition was performed by Xevo G2 QTof mass spectrometer coupled to the nanoACQUITY Ultra Performance Liquid Chromatography (both from Waters) controlled by MassLynx v.4.1 (Waters). Data collection was in data‐independent acquisition mode (LC–MSE), the mass ranges from 50 to 2000 m/z 11,12 …”

Section: Methodsmentioning

confidence: 99%

“…The supernatant from each sample was frozen at −80°C until proteomics analysis. 11,12 2.2.3 | Shotgun label-free quantitative proteomic analysis Saliva samples (v = 1 mL) were extracted and prepared individually by using an extraction solution containing 6 M urea, 2 M thiourea in 50 mM Ammonium Bicarbonate, pH 7.8, in a volume similar to the samples (1:1). Samples were vortexed for 10 min at 4°C, sonicated for 5 min and centrifuged at 20.817g for 10 min at 4°C for twice.…”

Section: Saliva Collectionmentioning

confidence: 99%

“…Furthermore, the study by Bell et al 10 showed an increase in myofibrillar protein FSR in older men, 24 and 48 h after RT and high‐intensity interval training (HIIT), but the increase was greater in RT. These studies may indicate the potential of each exercise protocol related, for example, to phenotypic adaptations 9–12 …”

Section: Introductionmentioning

confidence: 99%

mentioning

confidence: 99%

“…Saliva is one of these fluids that can be used because its collection is noninvasive and painless, 11,12 which has been often used as a means of obtaining the protein profile. 11,12,16 No previous study has applied the salivary proteomic to compare the effect of resistance exercise protocols, but saliva has been used to find biomarkers for other physiological and pathological systemic conditions. 17 Proteomic analysis can be advantageous because it brings an overview of the expression of all proteins in the sample, comparing it before and after exercise within the same patient in a reliable way.…”

mentioning

confidence: 99%

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Salivary proteomic profile of response to different resistance training protocols: A case report

Santos,

Filho,

Ventura

et al. 2024

Cell Biochemistry & Function

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Resistance training (RT) with blood flow restriction (BFR) or high intensity (HI) are effective to increase muscle mass. To understand this effect, techniques known as “omics” are used to identify possible biomarkers. This study analyzed the salivary proteomic profile of healthy individuals trained before and after two RT protocols both designed with eight exercises for upper‐ and lower‐limbs, one performed at low percentage of one‐maximum repetition (%1RM) with BFR technique, and other at high %1RM (HI) without BRF technique. Four healthy males between 18 and 28 years participated in the study. Stimulated saliva was collected before (BBFR/BHI) and immediately after (ABFR/AHI) the two RT protocols. All protein‐related processing was performed using label‐free proteomic. The difference in expression between groups was expressed as p < .05 for downregulated proteins and 1‐p > .95 for upregulated proteins. There was difference in salivary flow between ABFR and BBFR (p = .005). For HI, 87 proteins were found after the practice and 119 before. Three hemoglobin isoforms were increased in AHI compared with BHI. In the BFR comparison, 105 proteins were identified after (ABFR) and 70 before (BBFR). Among those increased ABFR, we highlight five hemoglobin isoforms and Deleted in malignant brain tumors 1 protein. Between ABFR and AHI, 17 isoforms of histones, Transaldolase, Transketolase, Glyceraldehyde‐3‐phosphate dehydrogenase, and Antileukoproteinase were decreased ABFR. For HI, there was an increase in proteins related to oxidative stress and metabolism of the musculoskeletal system, compared with BFR. HI seems to induce higher anabolic signaling to muscle mass increase and antiatherosclerotic effects.

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Section: Methodsmentioning

confidence: 99%

Section: Saliva Collectionmentioning

confidence: 99%