Proteomic profiles of Daphnia magna exposed to lead (II) acetate trihydrate and atrazine

Le, Vu Quynh Anh; Ahn, Ji‐Young; Heo, Mi Young; Cho, Sung‐Jin; Yoon, Hobaek; Park, Ji-Hoo; Ko, Jung Ho; Lee, Lyon; Han, Janet; Kim, Sang Yong; Kim, Yang‐Hoon; Min, Jiho

doi:10.1007/s13258-017-0551-6

Cited by 5 publications

(6 citation statements)

References 28 publications

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“…In a previous study, the LC20 values were (27.85 and 37.71) mg/L for Pb(CH 3 COO) 2 ·3H 2 O and atrazine, respectively. [ 11 ] Therefore, the result shows that D. magna is very sensitive to TCS.…”

Section: Resultsmentioning

confidence: 98%

“…Belonging to the actin‐depolymerizing factor family of proteins, Tsr includes a monomeric protein that binds to both filamentous (F) and monomeric (G) actin. [ 11 ] Tsr was identified as belonging to the Daphnia pulex species. However, in previous studies, Tsr was also found in D. magna .…”

Section: Resultsmentioning

confidence: 99%

“…An acute toxicity test was performed following a previously published protocol. [ 11 ] TCS, used as a toxic chemical, was purchased from Sigma–Aldrich. Proteomic analysis (from protein extraction to identification) was carried out according to a previously published protocol.…”

Section: Methodsmentioning

confidence: 99%

“…[ 10 ] Many studies have been conducted on the correlation between toxic substances and D. magna at various levels, from molecules, such as genes and proteins, to the whole body. [ 11 ] Likewise, comparing molecular responses in organisms exposed to toxic chemicals can provide an understanding of their interactions. [ 12 ] In addition, proteins showing expression patterns that respond to specific chemicals can be used as biomarkers for chemical detection.…”

Section: Introductionmentioning

confidence: 99%

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Detection of triclosan using 2‐domain hemoglobin promoter of Daphnia magna

Shin

et al. 2023

J Biochem & Molecular Tox

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Triclosan has been widely used as an antimicrobial agent. However, triclosan was found to cause toxicity, including muscle contraction disturbances, carcinogenesis, and endocrine disorders. In addition, it was found to affect central nervous system function adversely and even have ototoxic effects. Conventional methods for detecting such triclosan can be performed easily. However, the conventional detection methods are inadequate in precisely reflecting the impact of toxic substances on stressed organisms. Therefore, a test model for the toxic environment at the molecular level through the organism is needed. From that point of view, Daphnia magna is being used as a ubiquitous model. D. magna has the advantages of easy cultivation, a short lifespan and high reproductive capacity, and high sensitivity to chemicals. Therefore, the protein expression pattern of D. magna that appear in response to chemicals can be utilized as biomarkers for detecting specific chemicals. In this study, we characterized the proteomic response of D. magna following triclosan exposure via two‐dimensional (2D) gel electrophoresis. As a result, we confirmed that triclosan exposure completely suppressed D. magna 2‐domain hemoglobin protein and evaluated this protein as a biomarker for triclosan detection. We constructed the HeLa cells in which the GFP gene was controlled by D. magna 2‐domain hemoglobin promoter, which under normal conditions, expressed GFP, but upon triclosan exposure, suppressed GFP expression. Consequently, we consider that the HeLa cells containing the pBABE‐HBF3‐GFP plasmid developed in this study can be used as novel biomarkers for triclosan detection.

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Section: Resultsmentioning

confidence: 98%

Section: Resultsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Detection of triclosan using 2‐domain hemoglobin promoter of Daphnia magna

Shin

et al. 2023

J Biochem & Molecular Tox

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“…Subsequently, 20 immature brine shrimp nauplii were transferred into each test tube and the test tubes were kept under observation at room temperature. After 24 h, the proportion of mortality of the nauplii was evaluated for every concentration and the LC 50 values were calculated by the Probit Analysis [31].…”

Section: Brine Shrimp Lethality Testmentioning

confidence: 99%

In-vivo antiproliferative activity of Morus latifolia leaf and bark extracts against Ehrlich’s ascites carcinoma

et al. 2019

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Cancer is the second death causing disease all over the world and until today 100 different types of cancer have been identified whose treatment methods consist of serious side effects on human body. To reduce the frequency of adverse effects of cancer treatment, nowadays plant derived natural components are getting priority. The plant Morus latifolia is widely available in northern part of Bangladesh. The earlier researches suggested that popular varieties of some Morus sp. like Morus alba, Morus indica etc. have good anti-proliferative activity. Hence, this study was designed to evaluate the anti-proliferative activity of leaf and bark extracts of M. latifolia against Ehrlich's ascites carcinoma (EAC) in vivo. The leaf and bark extracts of M. latifolia were used in several bioassays including Brine shrimp lethality test, hemagglutination activity test, antioxidant activity test, and cell growth inhibition test. Besides, fluorescence microscopy was performed to study apoptotic features in EAC cells, and molecular analysis like real-time PCR were also conducted. The results of Brine shrimp lethality test, hemagglutination activity test, and antioxidant activity assay supported the cell growth inhibition capability of leaf and bark extracts which was confirmed by in vivo cell growth inhibition bioassay. Moreover, the experimental extracts were able to induce cell apoptotis through altering the expression pattern of Bcl-2 and Bax genes. All of the results of this study suggest that several noble compounds are present in M. latifolia plant extracts which are capable for healing cancer cells.

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Transgenerational Effects of Atrazine on Daphnia magna Based on Life-History Traits and Population Dynamics

Wang,

Li,

et al. 2024

Bull Environ Contam Toxicol

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Proteomic profiles of Daphnia magna exposed to lead (II) acetate trihydrate and atrazine

Cited by 5 publications

References 28 publications

Detection of triclosan using 2‐domain hemoglobin promoter of Daphnia magna

Detection of triclosan using 2‐domain hemoglobin promoter of Daphnia magna

In-vivo antiproliferative activity of Morus latifolia leaf and bark extracts against Ehrlich’s ascites carcinoma

Transgenerational Effects of Atrazine on Daphnia magna Based on Life-History Traits and Population Dynamics

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