Proteomic profiling of aphid Macrosiphum euphorbiae responses to host-plant-mediated stress induced by defoliation and water deficit

Nguyen, Thi Thuy An; Michaud, Dominique; Cloutier, Conrad

doi:10.1016/j.jinsphys.2007.02.018

Cited by 42 publications

(37 citation statements)

References 51 publications

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“…In potato, proteome studies have been carried out mainly on tuber tissue (Bauw et al 2006;Delaplace et al 2005;Lehesranta et al 2005Lehesranta et al , 2006 and in connection with investigation on the action of phytopathogens such as Pectobacterium atrosepticum (Coulthurst et al 2006, Mattinen et al 2007) and the aphid Macrosiphum euphorbiae (Nguyen et al 2007). The application of protein expression studies for cryopreservation is a new research area.…”

Section: Biochemical Analysis and Proteome Researchmentioning

confidence: 99%

Potato Shoot Tip Cryopreservation. A Review

2010

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Potato is one of the most important crops worldwide. Genetic resources of potato (Solanum tuberosum L. ssp. tuberosum) and related cultivated species are conserved through storage of tubers, in vitro plants and in cryopreservation. Cryopreservation, storage in or above liquid nitrogen, is the best option to maintain vegetatively propagated plants in the long term. The present review gives comprehensive information about various cryopreservation techniques for potato published from 1977 until the present. It discusses factors that affect the process and success of cryopreservation, such as donor culture conditions, preculture, cooling, warming and post-culture treatments. Studies are presented that analyse the histological and ultrastructural changes after different cryopreservation steps and the morphological pathways during regeneration of plants after rewarming. The maintenance of genetic stability in potato after cryopreservation has also been demonstrated by various phenotypic and molecular methods. The first thermal analyses on potato shoot tips are presented using differential scanning calorimetry to analyse the state of water during cooling and warming. Biochemical analyses of different compounds, such as soluble sugars and proteins, have been performed to understand and improve existing cryogenic methods. Potato is an example where successful virus elimination has been obtained via cryopreservation of shoot tips (cryotherapy). There are already cryopreserved collections of potato shoot tips in

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Section: Biochemical Analysis and Proteome Researchmentioning

confidence: 99%

Potato Shoot Tip Cryopreservation. A Review

2010

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“…Twodimensional gel electrophoresis (2-DE) and mass spectrometry (MS), in combination with accumulating database resources, provide powerful tools for characterizing proteins and their post-translational modifications in individual cells, tissues or whole organisms such as insects, in the context of experimentation. For example, these techniques have been recently applied to the aphid Macrosiphum euphorbiae in characterizing its protein profile following host plant-mediated stress (Nguyen et al, 2007). In the present study, we compared responses of the same aphid host to two differently compatible species of endoparasitoids, by relating the opposite outcome of their interactions with the host as revealed by proteomic profiling.…”

Section: Article In Pressmentioning

confidence: 99%

“…Protein extraction and 2-DE were performed as described by Nguyen et al (2007). Briefly, groups of 45 4th instar and adult aphids from each treatment were used for the analyses.…”

Section: Proteomic Analysesmentioning

confidence: 99%

“…Normalized volumes of each spot, reflecting protein expression levels, were first subjected to statistical analysis with a two-way ANOVA at Po0.05 for treatment effects. Because spot-by-spot ANOVA does not provide a comprehensive way to separate treatment effects, we also used a multivariate discriminant analysis approach (Huberty, 1994), whereby variation among all protein spots can be considered together in the same analysis (see Nguyen et al, 2007 for more details).…”

Section: Proteomic Analysesmentioning

confidence: 99%

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Proteomes of the aphid Macrosiphum euphorbiae in its resistance and susceptibility responses to differently compatible parasitoids

Nguyen

Boudreault

Michaud

et al. 2008

Insect Biochemistry and Molecular Biology

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“…Several studies have tested the effect of drought on plant-herbivore interactions (EnglishLoeb et al 1997;Inbar et al 2001;de Bruyn et al 2002;Scheirs and de Bruyn 2005;Nguyen et al 2007). Drought often resulted in decreased performance of the herbivore (growth, survival), mainly due to an increase in defensive compounds and a decrease in leaf nitrogen (Herms and Mattson 1992;EnglishLoeb et al 1997;Inbar et al 2001;de Bruyn et al 2002).…”

Section: Introductionmentioning

confidence: 99%

How do extreme drought and plant community composition affect host plant metabolites and herbivore performance?

Walter

Hein

Auge

et al. 2011

Arthropod-Plant Interactions

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Water availability and plant community composition alter plant nutrient availability and the accumulation of plant defence compounds therefore having an impact on herbivore performance. Combined effects of drought stress and plant community composition on leaf chemicals and herbivore performance are largely unexplored. The objective of our study was, therefore, to find out the impact of extreme drought and of plant community composition on plantherbivore interactions. Larvae of the generalist butterfly Spodoptera littoralis were reared on leaves of the grass Holcus lanatus which was grown in experimental communities, differing in species-and functional group richness. These communities were either subjected to extreme drought or remained under ambient climatic conditions. Drought decreased relative water content, soluble protein content, nitrogen and total phenol content and increased the content of carbohydrates in the grass. As a consequence, the larvae feeding on drought-exposed plants revealed a longer larval stage, increased pupal weight and higher adult eclosion rates. Plant community composition mainly caused changes to the defensive compounds of the grass, but also marginally affected protein and carbohydrate content. Larvae feeding on species-richest communities without legumes showed the highest mortality. Our findings imply that climate change that is projected to increase the frequency of severe droughts, as well as alter plant community compositions, is likely to affect arthropod-plant interactions through an alteration of leaf chemicals.

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Proteomic profiling of aphid Macrosiphum euphorbiae responses to host-plant-mediated stress induced by defoliation and water deficit

Cited by 42 publications

References 51 publications

Potato Shoot Tip Cryopreservation. A Review

Potato Shoot Tip Cryopreservation. A Review

Proteomes of the aphid Macrosiphum euphorbiae in its resistance and susceptibility responses to differently compatible parasitoids

How do extreme drought and plant community composition affect host plant metabolites and herbivore performance?

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