Proteomic profiling of hydatid fluid from pulmonary cystic echinococcosis

Abstract

Background Most cystic echinococcosis cases in Southern Brazil are caused by Echinococcus granulosus and Echinococcus ortleppi. Proteomic studies of helminths have increased our knowledge about the molecular survival strategies that are used by parasites. Here, we surveyed the protein content of the hydatid fluid compartment in E. granulosus and E. ortleppi pulmonary bovine cysts to better describe and compare their molecular arsenal at the host-parasite interface. Methods Hydatid fluid samples from three isolates of each species were analyzed using mass spectrometry-based proteomics (LC-MS/MS). In silico functional analyses of the identified proteins were performed to examine parasite survival strategies. Results The identified hydatid fluid protein profiles showed a predominance of parasite proteins compared to host proteins that infiltrate the cysts. We identified 280 parasitic proteins from E. granulosus and 251 from E. ortleppi, including 52 parasitic proteins that were common to all hydatid fluid samples. The in silico functional analysis revealed important molecular functions and processes that are active in pulmonary cystic echinococcosis, such as adhesion, extracellular structures organization, development regulation, signaling transduction, and enzyme activity. Conclusions The protein profiles described here provide evidence of important mechanisms related to basic cellular processes and functions that act at the host-parasite interface in cystic echinococcosis. The molecular tools used by E. granulosus and E. ortleppi for survival within the host are potential targets for new therapeutic approaches to treat cystic echinococcosis and other larval cestodiases. Graphical Abstract

Hydatid fluid from Echinococcus granulosus induces autophagy in dendritic cells and promotes polyfunctional T-cell responses

“…Sequence analysis indicated that both receptors encoded a type II transmembrane protein but lacked the conserved residues involved in calcium ion coordination and carbohydrate-binding found in the classical C-type lectins ( Huysamen et al., 2008 ; Gully et al., 2021 ). We have described that HF stimulation, through a still-unknown component, probably F-actin or another protein related to necrotic cells, described in proteomic analysis of HF ( Zeghir-Bouteldja et al., 2017 ; Schulz et al., 2018 ; Canton et al., 2021 ; dos Santos et al., 2022 ), induces downregulation of CLEC9A and CD205 in the cell membrane of BMDCs and this effect was not reversed with the use of EDTA.…”

Effects of protoscoleces excretory-secretory products of Echinococcus granulosus on hepatocyte growth, function, and glucose metabolism

“…In contrast to previous studies using hydatid fluid (HF)[22], we used ES products from E. granulosus PSCs to characterize their effects on hepatocytes. The ES obtained was actually purified HF and did not require concentration[23].…”

Omics: Applications related to diagnosis, treatment, prevention and control of parasitic diseases. Part II. Helminths

“…It is a transcriptomic approach for analyzing the effect of introducing oligonucleotides into the cells and probing the gene function loss [38] . Additionally, LC-MS/MS and in silico analysis of Echinococcus proteins revealed essential molecular functions related to pulmonary cystic echinococcosis [45] . These included adhesion, development regulation, enzymatic activity, and signaling transduction.…”

“…This study also identified collagen, glycoproteins and laminin for extracellular matrix construction and function, in addition to tyrosine protein kinase and glypican-1 essential for signalling pathways; as well as identification of antigen B that is responsible for nutrient uptake of host lipids and metalloproteases and cysteine proteases for proteolytic activity. Accordingly, these drug targets were suggested for development of novel therapeutics to treat cystic echinococcosis and other larval tapewormes [45] .…”