Proteomic profiling of lymphocytes in autoimmunity, inflammation and cancer

Zhou, Jiaxin; Zhu, Zhou; Bai, Chunxue; Sun, Hongmei; Wang, Xiangdong

doi:10.1186/1479-5876-12-6

Cited by 18 publications

(8 citation statements)

References 66 publications

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“…Apart from innate immunity, autophagy also plays an indispensable role in adaptive immunity, including the development and homeostasis of the immune system and antigen presentation [33]. Several tissue‐specific knockout models have been developed during the past few years to study the role of autophagy in T lymphocytes [34].…”

Section: Autophagy In Inflammationmentioning

confidence: 99%

Autophagy and inflammation

Qian

Fang

Wang

2017

Clinical & Translational Med

Self Cite

236

179

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Autophagy is a homeostatic mechanism involved in the disposal of damaged organelles, denatured proteins as well as invaded pathogens through a lysosomal degradation pathway. Recently, increasing evidences have demonstrated its role in both innate and adaptive immunity, and thereby influence the pathogenesis of inflammatory diseases. The detection of autophagy machinery facilitated the measurement of autophagy during physiological and pathophysiological processes. Autophagy plays critical roles in inflammation through influencing the development, homeostasis and survival of inflammatory cells, including macrophages, neutrophils and lymphocytes; effecting the transcription, processing and secretion of a number of cytokines, as well as being regulated by cytokines. Recently, autophagy‐dependent mechanisms have been studied in the pathogenesis of several inflammatory diseases, including infectious diseases, Crohn's disease, cystic fibrosis, pulmonary hypertension, chronic obstructive pulmonary diseases and so on. These studies suggested that modulation of autophagy might lead to therapeutic interventions for diseases associated with inflammation. Here we highlight recent advances in investigating the roles of autophagy in inflammation as well as inflammatory diseases.

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Section: Autophagy In Inflammationmentioning

confidence: 99%

Autophagy and inflammation

Qian

Fang

Wang

2017

Clinical & Translational Med

Self Cite

236

179

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“…Blood cells are interesting ex vivo models to study the pathophysiological state of diseases, and to predict the beneficial or toxic effect promoted by new therapies, with a high translationality to clinical studies by considering the patient’s specific characteristics. The reason is the fact that blood cells are altered in disease and can reflect the condition and state of different organs and tissues [ 1 ]. Thus, the study of the interaction of blood cells with medicines can provide us with an early indication of the effect of a certain therapy on the human body.…”

Section: Introductionmentioning

confidence: 99%

Assessment of gold nanoparticles on human peripheral blood cells by metabolic profiling with 1H-NMR spectroscopy, a novel translational approach on a patient-specific basis

Palomino‐Schätzlein¹,

Garcı́a²,

Gutiérrez-Carcedo³

et al. 2017

PLoS ONE

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Human peripheral blood cells are relevant ex vivo models for characterizing diseases and evaluating the pharmacological effects of therapeutic interventions, as they provide a close reflection of an individual pathophysiological state. In this work, a new approach to evaluate the impact of nanoparticles on the three main fractions of human peripheral blood cells by nuclear magnetic resonance spectroscopy is shown. Thus, a comprehensive protocol has been set-up including the separation of blood cells, their in vitro treatment with nanoparticles and the extraction and characterization of metabolites by nuclear magnetic resonance. This method was applied to assess the effect of gold nanoparticles, either coated with chitosan or supported on ceria, on peripheral blood cells from healthy individuals. A clear antioxidant effect was observed for chitosan-coated gold nanoparticles by a significant increase in reduced glutathione, that was much less pronounced for gold-cerium nanoparticles. In addition, the analysis revealed significant alterations of several other pathways, which were stronger for gold-cerium nanoparticles. These results are in accordance with the toxicological data previously reported for these materials, confirming the value of the current methodology.

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Section: Discussionmentioning

confidence: 99%

“…Lymphocytes play crucial roles in the immune defense of hosts against pathogens [ 20 ]. The analysis of proteomic profiles of lymphocytes can reveal more information about their functions and the reactions to external immune stimuli [ 20 ]. With our study, we have provided a comprehensive proteomic characterization of bovine peripheral blood derived lymphocytes for two different bovine immune phenotypes.…”

Section: Discussionmentioning

confidence: 99%

Bovine Peripheral Blood Derived Lymphocyte Proteome and Secretome Show Divergent Reaction of Bovine Immune Phenotypes after Stimulation with Pokeweed Mitogen

et al. 2022

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We recently identified a deviant bovine immune phenotype characterized by hyperproliferation of lymphocytes after polyclonal stimulation. This phenotype was first discovered in dams that responded to PregSure BVD vaccination by producing pathological antibodies, triggering the fatal disease “bovine neonatal pancytopenia” in calves. The aim of the study was to gain deeper insights into molecular processes occurring in lymphocytes of immune phenotypes and the effect on their secretome after immune stimulation. Two discovery proteomic experiments were performed with unstimulated and Pokeweed Mitogen (PWM) stimulated lymphocytes, using label-free LC-MS/MS. In lymphocytes, 2447 proteins were quantified, and 1204 proteins were quantified in the secretome. Quantitative proteome analysis of immune deviant and control samples after PWM stimulation revealed clear differences. The increase in abundance of IL17A, IL17F, IL8, CCL5, LRRC59, and CLIC4 was higher in controls through mitogenic stimulation. In contrast, the abundance of IFNγ, IL2, IL2RA, CD83, and CD200 increased significantly more in immune deviant lymphocytes. Additional pathway enrichment analysis of differentially secreted proteins also yielded fundamental differences between the immune phenotypes. Our study provides a comprehensive dataset, which gives novel insights into proteome changes of lymphocytes from different bovine immune phenotypes. These differences point to the development of diverse immune responses of bovine immune phenotypes after immune stimulation.

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Proteomic profiling of lymphocytes in autoimmunity, inflammation and cancer

Cited by 18 publications

References 66 publications

Autophagy and inflammation

Autophagy and inflammation

Assessment of gold nanoparticles on human peripheral blood cells by metabolic profiling with 1H-NMR spectroscopy, a novel translational approach on a patient-specific basis

Bovine Peripheral Blood Derived Lymphocyte Proteome and Secretome Show Divergent Reaction of Bovine Immune Phenotypes after Stimulation with Pokeweed Mitogen

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