Proteomic profiling of platelet signalling

Abstract

Platelets play a crucial role in hemostasis; activating and aggregating to arrest bleeding following vascular injury. Platelet activation is a complex and dynamic process, involving the co-ordination of numerous receptors to initiate shape change and aggregation. Under pathological conditions, alterations in the normal platelet response can favor a prothrombotic state and increase the risk of acute coronary syndromes (ACS). Receptor stimulation and the tyrosine phosphorylation of key signaling molecules underpin platelet activation in both hemostasis and cardiovascular disease. A lack of nucleus and low mRNA levels makes protein function the primary focus of platelet research. Advancements in proteomic technologies now allow for comprehensive analysis of the platelet proteome and its associated post-translational modifications. In this review, recent applications of proteomics in platelet signaling studies are discussed with particular focus on the elucidation of novel phosphorylation events following receptor activation.

Clinical‐grade quality platelet‐rich plasma releasate (PRP‐R/SRGF) from CaCl2‐activated platelet concentrates promoted expansion of mesenchymal stromal cells

“…PRP‐R/SRGF, defined supernatant rich in growth factors, was obtained by the simple addition of clinical‐grade CaCl 2 to platelet concentrates and in compliance with good laboratory practice (GLP) guidelines. PRP‐R/SRGF can be utilized in clinical settings and is cost‐effective since it does not require prolonged manipulations nor expensive consumables or technologies .…”